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Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling

Nichola J. Starr, Daniel Johnson Orcid Logo, Judata Wibawa, Ian Marlow, Mike Bell, David A. Barrett, David J. Scurr

Analytical Chemistry, Volume: 88, Issue: 8, Pages: 4400 - 4408

Swansea University Author: Daniel Johnson Orcid Logo

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DOI (Published version): 10.1021/acs.analchem.5b04872

Abstract

This work demonstrates the ability to detect changes in both quantity and spatial distribution of human stratum corneum (SC) lipids from samples collected in vivo. The SC functions as the predominant barrier to the body, protecting against the penetration of xenobiotic substances. Changes to the SC...

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Published in: Analytical Chemistry
ISSN: 0003-2700 1520-6882
Published: 2016
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URI: https://cronfa.swan.ac.uk/Record/cronfa33677
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spelling 2017-10-04T09:46:55.7383109 v2 33677 2017-05-16 Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling 4bdcc306062428d2715b0dd308cc092f 0000-0001-6921-0389 Daniel Johnson Daniel Johnson true false 2017-05-16 This work demonstrates the ability to detect changes in both quantity and spatial distribution of human stratum corneum (SC) lipids from samples collected in vivo. The SC functions as the predominant barrier to the body, protecting against the penetration of xenobiotic substances. Changes to the SC lipid composition have been associated with barrier impairment and consequent skin disorders, and it is therefore important to monitor and quantify changes to this structure. This work demonstrates the first reported use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) to assess physiological changes to human SC as a function of depth. This technique provides exceptional sensitivity and chemical specificity, allowing analysis of single tape stripped samples taken from volunteers. Using this methodology we were able to successfully identify chemical differences in human SC resulting from both intrinsic and extrinsic (photo) aging. Samples were collected from women of two age groups (under 27 and postmenopausal) and from two body sites with varying UV exposure (inner forearm and dorsal hand), and differences were identified using multivariate data analysis. The key finding was the significant aged-related increase and change in spatial distribution of the sterol cholesterol sulfate, a membrane stabilizing lipid. Significant changes in the prevalence of both lignoceric acid (C24:0) and hexacosanoic acid (C26:0) were also observed. This work describes previously unreported age-related chemical changes to human SC, providing an insight into aging mechanisms which may improve the design of both pharmaceutical and cosmetic topical products. Journal Article Analytical Chemistry 88 8 4400 4408 0003-2700 1520-6882 23 3 2016 2016-03-23 10.1021/acs.analchem.5b04872 http://dx.doi.org/10.1021/acs.analchem.5b04872 COLLEGE NANME COLLEGE CODE Swansea University 2017-10-04T09:46:55.7383109 2017-05-16T10:22:02.1769029 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Nichola J. Starr 1 Daniel Johnson 0000-0001-6921-0389 2 Judata Wibawa 3 Ian Marlow 4 Mike Bell 5 David A. Barrett 6 David J. Scurr 7
title Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling
spellingShingle Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling
Daniel Johnson
title_short Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling
title_full Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling
title_fullStr Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling
title_full_unstemmed Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling
title_sort Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling
author_id_str_mv 4bdcc306062428d2715b0dd308cc092f
author_id_fullname_str_mv 4bdcc306062428d2715b0dd308cc092f_***_Daniel Johnson
author Daniel Johnson
author2 Nichola J. Starr
Daniel Johnson
Judata Wibawa
Ian Marlow
Mike Bell
David A. Barrett
David J. Scurr
format Journal article
container_title Analytical Chemistry
container_volume 88
container_issue 8
container_start_page 4400
publishDate 2016
institution Swansea University
issn 0003-2700
1520-6882
doi_str_mv 10.1021/acs.analchem.5b04872
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
url http://dx.doi.org/10.1021/acs.analchem.5b04872
document_store_str 0
active_str 0
description This work demonstrates the ability to detect changes in both quantity and spatial distribution of human stratum corneum (SC) lipids from samples collected in vivo. The SC functions as the predominant barrier to the body, protecting against the penetration of xenobiotic substances. Changes to the SC lipid composition have been associated with barrier impairment and consequent skin disorders, and it is therefore important to monitor and quantify changes to this structure. This work demonstrates the first reported use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) to assess physiological changes to human SC as a function of depth. This technique provides exceptional sensitivity and chemical specificity, allowing analysis of single tape stripped samples taken from volunteers. Using this methodology we were able to successfully identify chemical differences in human SC resulting from both intrinsic and extrinsic (photo) aging. Samples were collected from women of two age groups (under 27 and postmenopausal) and from two body sites with varying UV exposure (inner forearm and dorsal hand), and differences were identified using multivariate data analysis. The key finding was the significant aged-related increase and change in spatial distribution of the sterol cholesterol sulfate, a membrane stabilizing lipid. Significant changes in the prevalence of both lignoceric acid (C24:0) and hexacosanoic acid (C26:0) were also observed. This work describes previously unreported age-related chemical changes to human SC, providing an insight into aging mechanisms which may improve the design of both pharmaceutical and cosmetic topical products.
published_date 2016-03-23T03:41:42Z
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score 11.012678

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