Journal article 1149 views 347 downloads
The effect of ventricular assist device-associated biomaterials on human blood leukocytes
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Swansea University Author:
Cathy Thornton
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DOI (Published version): 10.1002/jbm.b.33981
Abstract
Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the bi...
Published in: | Journal of Biomedical Materials Research Part B: Applied Biomaterials |
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ISSN: | 15524973 |
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2017
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URI: | https://cronfa.swan.ac.uk/Record/cronfa34948 |
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2017-11-21T17:04:49.1717793 v2 34948 2017-08-21 The effect of ventricular assist device-associated biomaterials on human blood leukocytes c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2017-08-21 BMS Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the biocompatibility of all biomaterials used is crucial to the success of medical devices.Methods: Biomaterials common in VADs - DLC: diamond-like carbon coated stainless steel; Sap: single-crystal sapphire; SiN: silicon nitride; Ti: titanium alloy; and ZTA: zirconia-toughened alumina - were tested for their biocompatibility through incubation with whole human blood for 2 hours with mild agitation. Blood was then removed and used for: complete cell counts; leukocyte activation and death, and the production of key inflammatory cytokines. All were compared to time 0 and an un-exposed 2 hour sample.Results: Monocyte numbers were lower after exposure to DLC, SiN and ZTA and monocytes showed evidence of activation with DLC, Sap, and SiN. Neutrophils and lymphocytes were unaffected.Conclusions: This approach allows comprehensive analysis of the potential blood damaging effects of biomaterials. Monocyte activation by DLC, Sap, ZTA and SiN warrants further investigation linking effects on this cell type to unfavourable inflammatory/thrombogenic responses to VADs and other blood handling devices. Journal Article Journal of Biomedical Materials Research Part B: Applied Biomaterials 15524973 Ventricular Assist Devices; Biomaterials; Human; Blood; Flow Cytometry 9 9 2017 2017-09-09 10.1002/jbm.b.33981 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2017-11-21T17:04:49.1717793 2017-08-21T14:43:06.2953962 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Gemma Radley 1 Ina Laura Pieper 2 Cathy Thornton 0000-0002-5153-573X 3 0034948-12092017120257.pdf 20170831_GRadley_JBMRBAcceptedScript.pdf 2017-09-12T12:02:57.1300000 Output 689641 application/pdf Accepted Manuscript true 2018-09-12T00:00:00.0000000 true eng |
title |
The effect of ventricular assist device-associated biomaterials on human blood leukocytes |
spellingShingle |
The effect of ventricular assist device-associated biomaterials on human blood leukocytes Cathy Thornton |
title_short |
The effect of ventricular assist device-associated biomaterials on human blood leukocytes |
title_full |
The effect of ventricular assist device-associated biomaterials on human blood leukocytes |
title_fullStr |
The effect of ventricular assist device-associated biomaterials on human blood leukocytes |
title_full_unstemmed |
The effect of ventricular assist device-associated biomaterials on human blood leukocytes |
title_sort |
The effect of ventricular assist device-associated biomaterials on human blood leukocytes |
author_id_str_mv |
c71a7a4be7361094d046d312202bce0c |
author_id_fullname_str_mv |
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton |
author |
Cathy Thornton |
author2 |
Gemma Radley Ina Laura Pieper Cathy Thornton |
format |
Journal article |
container_title |
Journal of Biomedical Materials Research Part B: Applied Biomaterials |
publishDate |
2017 |
institution |
Swansea University |
issn |
15524973 |
doi_str_mv |
10.1002/jbm.b.33981 |
college_str |
Faculty of Medicine, Health and Life Sciences |
hierarchytype |
|
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
hierarchy_parent_id |
facultyofmedicinehealthandlifesciences |
hierarchy_parent_title |
Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
document_store_str |
1 |
active_str |
0 |
description |
Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the biocompatibility of all biomaterials used is crucial to the success of medical devices.Methods: Biomaterials common in VADs - DLC: diamond-like carbon coated stainless steel; Sap: single-crystal sapphire; SiN: silicon nitride; Ti: titanium alloy; and ZTA: zirconia-toughened alumina - were tested for their biocompatibility through incubation with whole human blood for 2 hours with mild agitation. Blood was then removed and used for: complete cell counts; leukocyte activation and death, and the production of key inflammatory cytokines. All were compared to time 0 and an un-exposed 2 hour sample.Results: Monocyte numbers were lower after exposure to DLC, SiN and ZTA and monocytes showed evidence of activation with DLC, Sap, and SiN. Neutrophils and lymphocytes were unaffected.Conclusions: This approach allows comprehensive analysis of the potential blood damaging effects of biomaterials. Monocyte activation by DLC, Sap, ZTA and SiN warrants further investigation linking effects on this cell type to unfavourable inflammatory/thrombogenic responses to VADs and other blood handling devices. |
published_date |
2017-09-09T03:43:23Z |
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1763752021661319168 |
score |
11.021648 |