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The effect of ventricular assist device-associated biomaterials on human blood leukocytes / Gemma Radley; Ina Laura Pieper; Catherine A. Thornton

Journal of Biomedical Materials Research Part B: Applied Biomaterials

Swansea University Author: Thornton, Catherine

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DOI (Published version): 10.1002/jbm.b.33981

Abstract

Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the bi...

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Published in: Journal of Biomedical Materials Research Part B: Applied Biomaterials
ISSN: 15524973
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa34948
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first_indexed 2017-08-21T18:50:38Z
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spelling 2017-11-21T17:04:49Z v2 34948 2017-08-21 The effect of ventricular assist device-associated biomaterials on human blood leukocytes Catherine Thornton Catherine Thornton true 0000-0002-5153-573X false c71a7a4be7361094d046d312202bce0c 0789291f2bbb8aeb948db2352e425e6a 70js5AkMS5jPo3arCUY9c32HZhUyFASdV1DFdgIIhKs= 2017-08-21 PMSC Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the biocompatibility of all biomaterials used is crucial to the success of medical devices.Methods: Biomaterials common in VADs - DLC: diamond-like carbon coated stainless steel; Sap: single-crystal sapphire; SiN: silicon nitride; Ti: titanium alloy; and ZTA: zirconia-toughened alumina - were tested for their biocompatibility through incubation with whole human blood for 2 hours with mild agitation. Blood was then removed and used for: complete cell counts; leukocyte activation and death, and the production of key inflammatory cytokines. All were compared to time 0 and an un-exposed 2 hour sample.Results: Monocyte numbers were lower after exposure to DLC, SiN and ZTA and monocytes showed evidence of activation with DLC, Sap, and SiN. Neutrophils and lymphocytes were unaffected.Conclusions: This approach allows comprehensive analysis of the potential blood damaging effects of biomaterials. Monocyte activation by DLC, Sap, ZTA and SiN warrants further investigation linking effects on this cell type to unfavourable inflammatory/thrombogenic responses to VADs and other blood handling devices. Journal article Journal of Biomedical Materials Research Part B: Applied Biomaterials 15524973 Ventricular Assist Devices; Biomaterials; Human; Blood; Flow Cytometry 9 9 2017 2017-09-09 10.1002/jbm.b.33981 Swansea University Medical School Medicine CMED PMSC Microbes and immunity None 2017-11-21T17:04:49Z 2017-08-21T14:43:06Z Swansea University Medical School Medicine Gemma Radley 1 Ina Laura Pieper 2 Catherine A. Thornton 3 0034948-12092017120257.pdf 20170831_GRadley_JBMRBAcceptedScript.pdf 2017-09-12T12:02:57Z Output 689641 application/pdf AM true Updated Copyright 15/09/2017 2018-09-12T00:00:00 true eng
title The effect of ventricular assist device-associated biomaterials on human blood leukocytes
spellingShingle The effect of ventricular assist device-associated biomaterials on human blood leukocytes
Thornton, Catherine
title_short The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_full The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_fullStr The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_full_unstemmed The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_sort The effect of ventricular assist device-associated biomaterials on human blood leukocytes
author_id_str_mv c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv c71a7a4be7361094d046d312202bce0c_***_Thornton, Catherine
author Thornton, Catherine
author2 Gemma Radley
Ina Laura Pieper
Catherine A. Thornton
format Journal article
container_title Journal of Biomedical Materials Research Part B: Applied Biomaterials
publishDate 2017
institution Swansea University
issn 15524973
doi_str_mv 10.1002/jbm.b.33981
college_str Swansea University Medical School
hierarchytype
hierarchy_top_id swanseauniversitymedicalschool
hierarchy_top_title Swansea University Medical School
hierarchy_parent_id swanseauniversitymedicalschool
hierarchy_parent_title Swansea University Medical School
department_str Medicine{{{_:::_}}}Swansea University Medical School{{{_:::_}}}Medicine
document_store_str 1
active_str 1
researchgroup_str Microbes and immunity
description Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the biocompatibility of all biomaterials used is crucial to the success of medical devices.Methods: Biomaterials common in VADs - DLC: diamond-like carbon coated stainless steel; Sap: single-crystal sapphire; SiN: silicon nitride; Ti: titanium alloy; and ZTA: zirconia-toughened alumina - were tested for their biocompatibility through incubation with whole human blood for 2 hours with mild agitation. Blood was then removed and used for: complete cell counts; leukocyte activation and death, and the production of key inflammatory cytokines. All were compared to time 0 and an un-exposed 2 hour sample.Results: Monocyte numbers were lower after exposure to DLC, SiN and ZTA and monocytes showed evidence of activation with DLC, Sap, and SiN. Neutrophils and lymphocytes were unaffected.Conclusions: This approach allows comprehensive analysis of the potential blood damaging effects of biomaterials. Monocyte activation by DLC, Sap, ZTA and SiN warrants further investigation linking effects on this cell type to unfavourable inflammatory/thrombogenic responses to VADs and other blood handling devices.
published_date 2017-09-09T11:50:10Z
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