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CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury

Ashton Barnett-Vanes, Anna Sharrock, Theofano Eftaxiopoulou, Hari Arora Orcid Logo, Warren Macdonald, Anthony M.J. Bull, Sara M. Rankin

Journal of Trauma and Acute Care Surgery, Volume: 81, Issue: 3, Pages: 500 - 511

Swansea University Author: Hari Arora Orcid Logo

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DOI (Published version): 10.1097/TA.0000000000001116

Abstract

BACKGROUNDUnderstanding of the cellular immune response to primary blast lung injury (PBLI) is limited, with only the neutrophil response well documented. Moreover, its impact on the immune response in distal organs remains poorly understood. In this study, a rodent model of isolated primary blast i...

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Published in: Journal of Trauma and Acute Care Surgery
ISSN: 2163-0755
Published: 2016
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URI: https://cronfa.swan.ac.uk/Record/cronfa37125
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spelling 2019-03-24T00:01:48.0487811 v2 37125 2017-11-28 CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury ed7371c768e9746008a6807f9f7a1555 0000-0002-9790-0907 Hari Arora Hari Arora true false 2017-11-28 MEDE BACKGROUNDUnderstanding of the cellular immune response to primary blast lung injury (PBLI) is limited, with only the neutrophil response well documented. Moreover, its impact on the immune response in distal organs remains poorly understood. In this study, a rodent model of isolated primary blast injury was used to investigate the acute cellular immune response to isolated PBLI in the circulation and lung, including the monocyte response, and investigate distal subacute immune effects in the spleen and liver 6 hours after injury.METHODSRats were subjected to a shock wave (~135 kPa overpressure, 2 ms duration) inducing PBLI or sham procedure. Rat physiology was monitored, and at 1, 3, and 6 hours thereafter, blood, lung, and bronchoalveolar lavage fluid (BALF) were collected and analyzed by flow cytometry, enzyme-linked immunosorbent assay, and histologic examination. In addition, at 6 hours, spleen and liver were collected and analyzed by flow cytometry.RESULTSLung histology confirmed pulmonary barotrauma and inflammation. This was associated with rises in CXCL-1, interleukin 6 (IL-6), tumor necrosis factor α and albumin protein in the BALF. Significant acute increases in blood and lung neutrophils and CD43Lo/His48Hi (classical) monocytes/macrophages were detected. No significant changes were seen in blood or lung “nonclassical” monocyte and in natural killler, B, or T cells. In the BALF, significant increases were seen in neutrophils, CD43Lo monocyte-macrophages and monocyte chemoattractant protein-1. Significant increases in CD43Lo and Hi monocyte-macrophages were detected in the spleen at 6 hours.CONCLUSIONThis study reveals a robust and selective response of CD43Lo/His48Hi (classical) monocytes, in addition to neutrophils, in blood and lung tissue following PBLI. An increase in monocyte-macrophages was also observed in the spleen at 6 hours. This profile of immune cells in the blood and BALF could present a new research tool for translational studies seeking to monitor, assess, or attenuate the immune response in blast-injured patients. Journal Article Journal of Trauma and Acute Care Surgery 81 3 500 511 2163-0755 1 9 2016 2016-09-01 10.1097/TA.0000000000001116 https://spiral.imperial.ac.uk/handle/10044/1/41770 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2019-03-24T00:01:48.0487811 2017-11-28T13:41:53.4788887 Ashton Barnett-Vanes 1 Anna Sharrock 2 Theofano Eftaxiopoulou 3 Hari Arora 0000-0002-9790-0907 4 Warren Macdonald 5 Anthony M.J. Bull 6 Sara M. Rankin 7 37125__15788__7e143641bac8465c948842823154efdb.pdf barnettvanes2016.pdf 2019-11-05T09:55:44.0159346 Output 2266104 application/pdf Accepted Manuscript true 2019-11-05T00:00:00.0000000 false
title CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
spellingShingle CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
Hari Arora
title_short CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
title_full CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
title_fullStr CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
title_full_unstemmed CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
title_sort CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
author_id_str_mv ed7371c768e9746008a6807f9f7a1555
author_id_fullname_str_mv ed7371c768e9746008a6807f9f7a1555_***_Hari Arora
author Hari Arora
author2 Ashton Barnett-Vanes
Anna Sharrock
Theofano Eftaxiopoulou
Hari Arora
Warren Macdonald
Anthony M.J. Bull
Sara M. Rankin
format Journal article
container_title Journal of Trauma and Acute Care Surgery
container_volume 81
container_issue 3
container_start_page 500
publishDate 2016
institution Swansea University
issn 2163-0755
doi_str_mv 10.1097/TA.0000000000001116
url https://spiral.imperial.ac.uk/handle/10044/1/41770
document_store_str 1
active_str 0
description BACKGROUNDUnderstanding of the cellular immune response to primary blast lung injury (PBLI) is limited, with only the neutrophil response well documented. Moreover, its impact on the immune response in distal organs remains poorly understood. In this study, a rodent model of isolated primary blast injury was used to investigate the acute cellular immune response to isolated PBLI in the circulation and lung, including the monocyte response, and investigate distal subacute immune effects in the spleen and liver 6 hours after injury.METHODSRats were subjected to a shock wave (~135 kPa overpressure, 2 ms duration) inducing PBLI or sham procedure. Rat physiology was monitored, and at 1, 3, and 6 hours thereafter, blood, lung, and bronchoalveolar lavage fluid (BALF) were collected and analyzed by flow cytometry, enzyme-linked immunosorbent assay, and histologic examination. In addition, at 6 hours, spleen and liver were collected and analyzed by flow cytometry.RESULTSLung histology confirmed pulmonary barotrauma and inflammation. This was associated with rises in CXCL-1, interleukin 6 (IL-6), tumor necrosis factor α and albumin protein in the BALF. Significant acute increases in blood and lung neutrophils and CD43Lo/His48Hi (classical) monocytes/macrophages were detected. No significant changes were seen in blood or lung “nonclassical” monocyte and in natural killler, B, or T cells. In the BALF, significant increases were seen in neutrophils, CD43Lo monocyte-macrophages and monocyte chemoattractant protein-1. Significant increases in CD43Lo and Hi monocyte-macrophages were detected in the spleen at 6 hours.CONCLUSIONThis study reveals a robust and selective response of CD43Lo/His48Hi (classical) monocytes, in addition to neutrophils, in blood and lung tissue following PBLI. An increase in monocyte-macrophages was also observed in the spleen at 6 hours. This profile of immune cells in the blood and BALF could present a new research tool for translational studies seeking to monitor, assess, or attenuate the immune response in blast-injured patients.
published_date 2016-09-01T03:46:38Z
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