Journal article 1350 views 222 downloads
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury
Ashton Barnett-Vanes,
Anna Sharrock,
Theofano Eftaxiopoulou,
Hari Arora ,
Warren Macdonald,
Anthony M.J. Bull,
Sara M. Rankin
Journal of Trauma and Acute Care Surgery, Volume: 81, Issue: 3, Pages: 500 - 511
Swansea University Author: Hari Arora
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DOI (Published version): 10.1097/TA.0000000000001116
Abstract
BACKGROUNDUnderstanding of the cellular immune response to primary blast lung injury (PBLI) is limited, with only the neutrophil response well documented. Moreover, its impact on the immune response in distal organs remains poorly understood. In this study, a rodent model of isolated primary blast i...
Published in: | Journal of Trauma and Acute Care Surgery |
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ISSN: | 2163-0755 |
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2016
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URI: | https://cronfa.swan.ac.uk/Record/cronfa37125 |
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<?xml version="1.0"?><rfc1807><datestamp>2019-03-24T00:01:48.0487811</datestamp><bib-version>v2</bib-version><id>37125</id><entry>2017-11-28</entry><title>CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury</title><swanseaauthors><author><sid>ed7371c768e9746008a6807f9f7a1555</sid><ORCID>0000-0002-9790-0907</ORCID><firstname>Hari</firstname><surname>Arora</surname><name>Hari Arora</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2017-11-28</date><deptcode>EAAS</deptcode><abstract>BACKGROUNDUnderstanding of the cellular immune response to primary blast lung injury (PBLI) is limited, with only the neutrophil response well documented. Moreover, its impact on the immune response in distal organs remains poorly understood. In this study, a rodent model of isolated primary blast injury was used to investigate the acute cellular immune response to isolated PBLI in the circulation and lung, including the monocyte response, and investigate distal subacute immune effects in the spleen and liver 6 hours after injury.METHODSRats were subjected to a shock wave (~135 kPa overpressure, 2 ms duration) inducing PBLI or sham procedure. Rat physiology was monitored, and at 1, 3, and 6 hours thereafter, blood, lung, and bronchoalveolar lavage fluid (BALF) were collected and analyzed by flow cytometry, enzyme-linked immunosorbent assay, and histologic examination. In addition, at 6 hours, spleen and liver were collected and analyzed by flow cytometry.RESULTSLung histology confirmed pulmonary barotrauma and inflammation. This was associated with rises in CXCL-1, interleukin 6 (IL-6), tumor necrosis factor α and albumin protein in the BALF. Significant acute increases in blood and lung neutrophils and CD43Lo/His48Hi (classical) monocytes/macrophages were detected. No significant changes were seen in blood or lung “nonclassical” monocyte and in natural killler, B, or T cells. In the BALF, significant increases were seen in neutrophils, CD43Lo monocyte-macrophages and monocyte chemoattractant protein-1. Significant increases in CD43Lo and Hi monocyte-macrophages were detected in the spleen at 6 hours.CONCLUSIONThis study reveals a robust and selective response of CD43Lo/His48Hi (classical) monocytes, in addition to neutrophils, in blood and lung tissue following PBLI. An increase in monocyte-macrophages was also observed in the spleen at 6 hours. This profile of immune cells in the blood and BALF could present a new research tool for translational studies seeking to monitor, assess, or attenuate the immune response in blast-injured patients.</abstract><type>Journal Article</type><journal>Journal of Trauma and Acute Care Surgery</journal><volume>81</volume><journalNumber>3</journalNumber><paginationStart>500</paginationStart><paginationEnd>511</paginationEnd><publisher/><issnPrint>2163-0755</issnPrint><keywords/><publishedDay>1</publishedDay><publishedMonth>9</publishedMonth><publishedYear>2016</publishedYear><publishedDate>2016-09-01</publishedDate><doi>10.1097/TA.0000000000001116</doi><url>https://spiral.imperial.ac.uk/handle/10044/1/41770</url><notes/><college>COLLEGE NANME</college><department>Engineering and Applied Sciences School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>EAAS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2019-03-24T00:01:48.0487811</lastEdited><Created>2017-11-28T13:41:53.4788887</Created><authors><author><firstname>Ashton</firstname><surname>Barnett-Vanes</surname><order>1</order></author><author><firstname>Anna</firstname><surname>Sharrock</surname><order>2</order></author><author><firstname>Theofano</firstname><surname>Eftaxiopoulou</surname><order>3</order></author><author><firstname>Hari</firstname><surname>Arora</surname><orcid>0000-0002-9790-0907</orcid><order>4</order></author><author><firstname>Warren</firstname><surname>Macdonald</surname><order>5</order></author><author><firstname>Anthony M.J.</firstname><surname>Bull</surname><order>6</order></author><author><firstname>Sara M.</firstname><surname>Rankin</surname><order>7</order></author></authors><documents><document><filename>37125__15788__7e143641bac8465c948842823154efdb.pdf</filename><originalFilename>barnettvanes2016.pdf</originalFilename><uploaded>2019-11-05T09:55:44.0159346</uploaded><type>Output</type><contentLength>2266104</contentLength><contentType>application/pdf</contentType><version>Accepted Manuscript</version><cronfaStatus>true</cronfaStatus><embargoDate>2019-11-05T00:00:00.0000000</embargoDate><copyrightCorrect>false</copyrightCorrect></document></documents><OutputDurs/></rfc1807> |
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2019-03-24T00:01:48.0487811 v2 37125 2017-11-28 CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury ed7371c768e9746008a6807f9f7a1555 0000-0002-9790-0907 Hari Arora Hari Arora true false 2017-11-28 EAAS BACKGROUNDUnderstanding of the cellular immune response to primary blast lung injury (PBLI) is limited, with only the neutrophil response well documented. Moreover, its impact on the immune response in distal organs remains poorly understood. In this study, a rodent model of isolated primary blast injury was used to investigate the acute cellular immune response to isolated PBLI in the circulation and lung, including the monocyte response, and investigate distal subacute immune effects in the spleen and liver 6 hours after injury.METHODSRats were subjected to a shock wave (~135 kPa overpressure, 2 ms duration) inducing PBLI or sham procedure. Rat physiology was monitored, and at 1, 3, and 6 hours thereafter, blood, lung, and bronchoalveolar lavage fluid (BALF) were collected and analyzed by flow cytometry, enzyme-linked immunosorbent assay, and histologic examination. In addition, at 6 hours, spleen and liver were collected and analyzed by flow cytometry.RESULTSLung histology confirmed pulmonary barotrauma and inflammation. This was associated with rises in CXCL-1, interleukin 6 (IL-6), tumor necrosis factor α and albumin protein in the BALF. Significant acute increases in blood and lung neutrophils and CD43Lo/His48Hi (classical) monocytes/macrophages were detected. No significant changes were seen in blood or lung “nonclassical” monocyte and in natural killler, B, or T cells. In the BALF, significant increases were seen in neutrophils, CD43Lo monocyte-macrophages and monocyte chemoattractant protein-1. Significant increases in CD43Lo and Hi monocyte-macrophages were detected in the spleen at 6 hours.CONCLUSIONThis study reveals a robust and selective response of CD43Lo/His48Hi (classical) monocytes, in addition to neutrophils, in blood and lung tissue following PBLI. An increase in monocyte-macrophages was also observed in the spleen at 6 hours. This profile of immune cells in the blood and BALF could present a new research tool for translational studies seeking to monitor, assess, or attenuate the immune response in blast-injured patients. Journal Article Journal of Trauma and Acute Care Surgery 81 3 500 511 2163-0755 1 9 2016 2016-09-01 10.1097/TA.0000000000001116 https://spiral.imperial.ac.uk/handle/10044/1/41770 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University 2019-03-24T00:01:48.0487811 2017-11-28T13:41:53.4788887 Ashton Barnett-Vanes 1 Anna Sharrock 2 Theofano Eftaxiopoulou 3 Hari Arora 0000-0002-9790-0907 4 Warren Macdonald 5 Anthony M.J. Bull 6 Sara M. Rankin 7 37125__15788__7e143641bac8465c948842823154efdb.pdf barnettvanes2016.pdf 2019-11-05T09:55:44.0159346 Output 2266104 application/pdf Accepted Manuscript true 2019-11-05T00:00:00.0000000 false |
title |
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury |
spellingShingle |
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury Hari Arora |
title_short |
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury |
title_full |
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury |
title_fullStr |
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury |
title_full_unstemmed |
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury |
title_sort |
CD43Lo classical monocytes participate in the cellular immune response to isolated primary blast lung injury |
author_id_str_mv |
ed7371c768e9746008a6807f9f7a1555 |
author_id_fullname_str_mv |
ed7371c768e9746008a6807f9f7a1555_***_Hari Arora |
author |
Hari Arora |
author2 |
Ashton Barnett-Vanes Anna Sharrock Theofano Eftaxiopoulou Hari Arora Warren Macdonald Anthony M.J. Bull Sara M. Rankin |
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Journal article |
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Journal of Trauma and Acute Care Surgery |
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81 |
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500 |
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2016 |
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Swansea University |
issn |
2163-0755 |
doi_str_mv |
10.1097/TA.0000000000001116 |
url |
https://spiral.imperial.ac.uk/handle/10044/1/41770 |
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description |
BACKGROUNDUnderstanding of the cellular immune response to primary blast lung injury (PBLI) is limited, with only the neutrophil response well documented. Moreover, its impact on the immune response in distal organs remains poorly understood. In this study, a rodent model of isolated primary blast injury was used to investigate the acute cellular immune response to isolated PBLI in the circulation and lung, including the monocyte response, and investigate distal subacute immune effects in the spleen and liver 6 hours after injury.METHODSRats were subjected to a shock wave (~135 kPa overpressure, 2 ms duration) inducing PBLI or sham procedure. Rat physiology was monitored, and at 1, 3, and 6 hours thereafter, blood, lung, and bronchoalveolar lavage fluid (BALF) were collected and analyzed by flow cytometry, enzyme-linked immunosorbent assay, and histologic examination. In addition, at 6 hours, spleen and liver were collected and analyzed by flow cytometry.RESULTSLung histology confirmed pulmonary barotrauma and inflammation. This was associated with rises in CXCL-1, interleukin 6 (IL-6), tumor necrosis factor α and albumin protein in the BALF. Significant acute increases in blood and lung neutrophils and CD43Lo/His48Hi (classical) monocytes/macrophages were detected. No significant changes were seen in blood or lung “nonclassical” monocyte and in natural killler, B, or T cells. In the BALF, significant increases were seen in neutrophils, CD43Lo monocyte-macrophages and monocyte chemoattractant protein-1. Significant increases in CD43Lo and Hi monocyte-macrophages were detected in the spleen at 6 hours.CONCLUSIONThis study reveals a robust and selective response of CD43Lo/His48Hi (classical) monocytes, in addition to neutrophils, in blood and lung tissue following PBLI. An increase in monocyte-macrophages was also observed in the spleen at 6 hours. This profile of immune cells in the blood and BALF could present a new research tool for translational studies seeking to monitor, assess, or attenuate the immune response in blast-injured patients. |
published_date |
2016-09-01T07:15:00Z |
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11.047306 |