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Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal
Ledys S. Copete-Pertuz,
Jersson Plácido,
Efraím A. Serna-Galvis,
Ricardo A. Torres-Palma,
Amanda Mora,
Jersson Placido Escobar 
Science of The Total Environment, Volume: 630, Pages: 1195 - 1204
Swansea University Author:
Jersson Placido Escobar
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DOI (Published version): 10.1016/j.scitotenv.2018.02.244
Abstract
In this work, Leptosphaerulina sp. (a Colombian native fungus) significantly removed three Isoxazolyl-Penicillin antibiotics (IP): oxacillin (OXA, 16000 µg L-1), cloxacillin (CLX, 17500 µg L-1) and dicloxacillin (DCX, 19000 µg L-1) from water. The biological treatment was performed at pH 5.6, 28 °C,...
| Published in: | Science of The Total Environment |
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| ISSN: | 00489697 |
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2018
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2018-03-08T12:17:00.2361712 v2 38842 2018-02-22 Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal ee053a8f277a0822f4dbb10470a03ef8 0000-0002-2070-3366 Jersson Placido Escobar Jersson Placido Escobar true false 2018-02-22 PMSC In this work, Leptosphaerulina sp. (a Colombian native fungus) significantly removed three Isoxazolyl-Penicillin antibiotics (IP): oxacillin (OXA, 16000 µg L-1), cloxacillin (CLX, 17500 µg L-1) and dicloxacillin (DCX, 19000 µg L-1) from water. The biological treatment was performed at pH 5.6, 28 °C, and 160 rpm for 15 days. The biotransformation proccess and lack of toxicity of the final solutions (antibacterial activity (AA) and cytotoxicity) were tested. The role of enzymes in IP removal was analysed through in vitro studies with enzymatic extracts (crude and pre-purified) from Leptosphaerulina sp., commercial enzymes and enzymatic inhibitors. Futhermore, the applicabililty of mycoremediation process to a complex matrix (simulated hospital wastewater) was evaluated. IP were considerably abated by the fungus, OXA was the fastest degraded (day 6), followed by CLX (day 7) and DCX (day 8). Antibiotics biodegradation was associated to laccase and versatile peroxidase action. Assays using commercial enzymes (i.e. laccase from Trametes versicolor and horseradish peroxidase) and inhibitors (EDTA, NaCl, sodium acetate, manganese (II) ions) confirmed the significant role of enzymatic transformation. Whereas, biomass sorption was not an important process in the antibiotics elimination. Evaluation of AA against Staphylococcus aureus ATCC 6538 revealed that Leptosphaerulina sp. also eliminated the AA. In addition, the cytotoxicity assay (MTT) on the HepG2 cell line demonstrated that the IP final solutions were non-toxic. Finally, Leptosphaerulina sp. eliminated OXA and its AA from synthetic hospital wastewater at 6 days. All these results evidenced the potential of Leptosphaerulina sp. mycoremediation as a novel environmentally friendly process for the removal of IP from aqueous systems. Journal Article Science of The Total Environment 630 1195 1204 00489697 White-rot fungi; Ligninolytic enzymes; Antibiotics degradation; Biotransformation; Wastewater treatment; Hospital wastewaters 15 7 2018 2018-07-15 10.1016/j.scitotenv.2018.02.244 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University 2018-03-08T12:17:00.2361712 2018-02-22T12:05:47.2075060 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Ledys S. Copete-Pertuz 1 Jersson Plácido 2 Efraím A. Serna-Galvis 3 Ricardo A. Torres-Palma 4 Amanda Mora 5 Jersson Placido Escobar 0000-0002-2070-3366 6 0038842-22022018133503.pdf Revisedmanuscriptwithtablesandfigures.pdf 2018-02-22T13:35:03.7500000 Output 916060 application/pdf Accepted Manuscript true 2019-03-07T00:00:00.0000000 Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND). true eng |
| title |
Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal |
| spellingShingle |
Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal Jersson Placido Escobar |
| title_short |
Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal |
| title_full |
Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal |
| title_fullStr |
Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal |
| title_full_unstemmed |
Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal |
| title_sort |
Elimination of Isoxazolyl-Penicillins antibiotics in waters by the ligninolytic native Colombian strain Leptosphaerulina sp. considerations on biodegradation process and antimicrobial activity removal |
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ee053a8f277a0822f4dbb10470a03ef8 |
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ee053a8f277a0822f4dbb10470a03ef8_***_Jersson Placido Escobar |
| author |
Jersson Placido Escobar |
| author2 |
Ledys S. Copete-Pertuz Jersson Plácido Efraím A. Serna-Galvis Ricardo A. Torres-Palma Amanda Mora Jersson Placido Escobar |
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Journal article |
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Science of The Total Environment |
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630 |
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1195 |
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2018 |
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Swansea University |
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00489697 |
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10.1016/j.scitotenv.2018.02.244 |
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Faculty of Medicine, Health and Life Sciences |
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| description |
In this work, Leptosphaerulina sp. (a Colombian native fungus) significantly removed three Isoxazolyl-Penicillin antibiotics (IP): oxacillin (OXA, 16000 µg L-1), cloxacillin (CLX, 17500 µg L-1) and dicloxacillin (DCX, 19000 µg L-1) from water. The biological treatment was performed at pH 5.6, 28 °C, and 160 rpm for 15 days. The biotransformation proccess and lack of toxicity of the final solutions (antibacterial activity (AA) and cytotoxicity) were tested. The role of enzymes in IP removal was analysed through in vitro studies with enzymatic extracts (crude and pre-purified) from Leptosphaerulina sp., commercial enzymes and enzymatic inhibitors. Futhermore, the applicabililty of mycoremediation process to a complex matrix (simulated hospital wastewater) was evaluated. IP were considerably abated by the fungus, OXA was the fastest degraded (day 6), followed by CLX (day 7) and DCX (day 8). Antibiotics biodegradation was associated to laccase and versatile peroxidase action. Assays using commercial enzymes (i.e. laccase from Trametes versicolor and horseradish peroxidase) and inhibitors (EDTA, NaCl, sodium acetate, manganese (II) ions) confirmed the significant role of enzymatic transformation. Whereas, biomass sorption was not an important process in the antibiotics elimination. Evaluation of AA against Staphylococcus aureus ATCC 6538 revealed that Leptosphaerulina sp. also eliminated the AA. In addition, the cytotoxicity assay (MTT) on the HepG2 cell line demonstrated that the IP final solutions were non-toxic. Finally, Leptosphaerulina sp. eliminated OXA and its AA from synthetic hospital wastewater at 6 days. All these results evidenced the potential of Leptosphaerulina sp. mycoremediation as a novel environmentally friendly process for the removal of IP from aqueous systems. |
| published_date |
2018-07-15T03:49:15Z |
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1763752390768459776 |
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11.035655 |