Journal article 2160 views 537 downloads
Hemocyanin-derived phenoloxidase reaction products display anti-infective properties
Christopher Coates,
James Talbot
Developmental & Comparative Immunology, Volume: 86, Pages: 47 - 51
Swansea University Author: Christopher Coates
DOI (Published version): 10.1016/j.dci.2018.04.017
Abstract
Hemocyanin is a multi-functional protein located in the hemolymph (blood) of certain arthropods and molluscs. In addition to its well-defined role in oxygen transport, hemocyanin can be converted into a phenoloxidase-like enzyme. Herein, we tested the antimicrobial properties of horseshoe crab (Limu...
| Published in: | Developmental & Comparative Immunology |
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| ISSN: | 0145305X |
| Published: |
2018
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa39536 |
| Abstract: |
Hemocyanin is a multi-functional protein located in the hemolymph (blood) of certain arthropods and molluscs. In addition to its well-defined role in oxygen transport, hemocyanin can be converted into a phenoloxidase-like enzyme. Herein, we tested the antimicrobial properties of horseshoe crab (Limulus polyphemus) hemocyanin-derived phenoloxidase reaction products using broad ranges of phenolic substrates (e.g. L-DOPA) and microbial targets (Gram-positive/negative bacteria, yeast). The enzyme-catalysed turnover of several substrates generated (by)products that reduced significantly the number of colony forming units. Microbicidal effects of hemocyanin-derived phenoloxidase were thwarted by the inhibitor phenylthiourea. Data presentedhere further support a role for hemocyanin in invertebrate innate immunity. |
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| College: |
Faculty of Science and Engineering |
| Start Page: |
47 |
| End Page: |
51 |