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Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots / Rachel Churm

Swansea University Author: Rachel Churm

DOI (Published version): 10.23889/SUthesis.40925

Abstract

The 28 amino acid hormone, ghrelin, has been found to have various effects on metabolism. This thesis focuses on the pathways integrated into ghrelin’s effect within adipocytes and adipose tissue depots of those with and without Type 2 diabetes. To determine whether acyl-ghrelin plays a role in medi...

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Published: 2018
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa40925
first_indexed 2018-07-03T19:34:30Z
last_indexed 2020-09-02T03:04:59Z
id cronfa40925
recordtype RisThesis
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spelling 2020-09-01T16:44:32.5294551 v2 40925 2018-07-03 Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots d4c0495092eedcc17399bd66491d5ee4 NULL Rachel Churm Rachel Churm true true 2018-07-03 The 28 amino acid hormone, ghrelin, has been found to have various effects on metabolism. This thesis focuses on the pathways integrated into ghrelin’s effect within adipocytes and adipose tissue depots of those with and without Type 2 diabetes. To determine whether acyl-ghrelin plays a role in mediating the metabolic state in an in vitro and ex vivo setting this thesis investigates cellular mechanisms via the analysis of: lipid staining, lipid retention gene expression pathway, inflammatory marker levels and determination of oxidative burden. This project confirms and translates previous murine model findings that establishes a mediatory role for acyl-ghrelin within lipid retention. Furthermore, this mechanism is influenced and magnified within the presence of hyperglycaemia, indicating that the impact of glucose metabolism on acyl-ghrelin and lipid homeostasis may result in the deterioration of dyslipidaemia. In addition to novel findings relating to lipid retention, results indicate that acyl-ghrelin also impacts the inflammatory state. Acyl-ghrelin exposure resulted in a marked decrease in pro-inflammatory marker IL-6, and ghrelin mRNA expression was associated with an increase in IL-10 and total antioxidant status. The promotion of the inflammatory state in the presence of acyl-ghrelin may yield novel therapeutic avenues for acyl-ghrelin combination treatment in the amelioration of the low-grade inflammation present within Type 2 diabetes. E-Thesis Adipose tissue, Obesity, Type 2 diabetes, Ghrelin. 31 12 2018 2018-12-31 10.23889/SUthesis.40925 A selection of third party content is redacted or is partially redacted from this thesis. COLLEGE NANME Swansea University Medical School COLLEGE CODE Swansea University Doctoral Ph.D Health Care Research Wales HS-40-14 2020-09-01T16:44:32.5294551 2018-07-03T15:13:23.0599200 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Rachel Churm NULL 1 0040925-03072018153402.pdf Churm_Rachel_Final_Redacted.pdf 2018-07-03T15:34:02.0100000 Output 7460959 application/pdf Redacted version - open access true 2020-05-01T00:00:00.0000000 true
title Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots
spellingShingle Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots
Rachel Churm
title_short Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots
title_full Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots
title_fullStr Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots
title_full_unstemmed Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots
title_sort Acyl-ghrelin mediated lipid retention and inflammation within in vitro and ex vivo adipose depots
author_id_str_mv d4c0495092eedcc17399bd66491d5ee4
author_id_fullname_str_mv d4c0495092eedcc17399bd66491d5ee4_***_Rachel Churm
author Rachel Churm
author2 Rachel Churm
format E-Thesis
publishDate 2018
institution Swansea University
doi_str_mv 10.23889/SUthesis.40925
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description The 28 amino acid hormone, ghrelin, has been found to have various effects on metabolism. This thesis focuses on the pathways integrated into ghrelin’s effect within adipocytes and adipose tissue depots of those with and without Type 2 diabetes. To determine whether acyl-ghrelin plays a role in mediating the metabolic state in an in vitro and ex vivo setting this thesis investigates cellular mechanisms via the analysis of: lipid staining, lipid retention gene expression pathway, inflammatory marker levels and determination of oxidative burden. This project confirms and translates previous murine model findings that establishes a mediatory role for acyl-ghrelin within lipid retention. Furthermore, this mechanism is influenced and magnified within the presence of hyperglycaemia, indicating that the impact of glucose metabolism on acyl-ghrelin and lipid homeostasis may result in the deterioration of dyslipidaemia. In addition to novel findings relating to lipid retention, results indicate that acyl-ghrelin also impacts the inflammatory state. Acyl-ghrelin exposure resulted in a marked decrease in pro-inflammatory marker IL-6, and ghrelin mRNA expression was associated with an increase in IL-10 and total antioxidant status. The promotion of the inflammatory state in the presence of acyl-ghrelin may yield novel therapeutic avenues for acyl-ghrelin combination treatment in the amelioration of the low-grade inflammation present within Type 2 diabetes.
published_date 2018-12-31T13:24:57Z
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score 11.048149

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