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On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling. / LEI JIANG

Swansea University Author: LEI JIANG

Abstract

The human gut microbiota is considered to be a highly specialized organ providing nourishment, regulating epithelial cell development, modulating innate immune responses and colonization resistances, and it significantly impacts human health and disease. Dispite of being extensively studied for seve...

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Published: 2013
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa42214
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first_indexed 2018-08-02T18:54:10Z
last_indexed 2020-09-04T03:03:01Z
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spelling 2020-09-03T08:53:48.3426422 v2 42214 2018-08-02 On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling. 83091f58166b25607411a755706bd449 LEI JIANG LEI JIANG true false 2018-08-02 The human gut microbiota is considered to be a highly specialized organ providing nourishment, regulating epithelial cell development, modulating innate immune responses and colonization resistances, and it significantly impacts human health and disease. Dispite of being extensively studied for several decades, the functionality of the microbiota colonization in the human gastrointestinal tract and the mechanisms of the interactions between the host and bacteria are still poorly understood. This research follows a novel and unique approach, which combines the complementary strengths of in vitro experiment, in vivo study and mathematical modelling. The work undertaken has three emphases: 1) probiotic strains and their impact on human health; 2) the development of gut microbiota in infants; 3) quantification of human gut microbial ecosystem at both the species level and the system level. In the first part of this research, a versatile anaerobic continuous culture platform was implemented following a novel and unique design, which allows easy and continuous sampling and monitoring of microbial growth. A number of carefully planned in vitro experiments have been conducted to investigate the growth and competition of probiotic strains under different culture conditions. These in vitro experiments improve the understanding for the growth behaviour of the specific probiotic strains. The second part of this project analyzed 50 faecal samples collected from 9 healthy infants with administration of probiotic strains and placebo. The analysis is based on the 454-pyrosequencing technology, which reveals the complete profiles of gut microbiota in these infants and confirmed the modulation effect of the specific probiotic strains. The last part of this research focused on the development of mathematical and computational models of human gut microbial ecosystem. The outcome from this part of the research includes: a) a new bacterial growth model that overcomes the parodox of competitative exclusion caused by previous models; b) a versatile computational framework to simulate in vitro fermentation experiments; and c) a comprehensive mathematical model for human gut and gut microbiota that is the first model for its nature. E-Thesis Microbiology. 31 12 2013 2013-12-31 COLLEGE NANME COLLEGE CODE Swansea University Doctoral Ph.D 2020-09-03T08:53:48.3426422 2018-08-02T16:24:28.4485804 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised LEI JIANG 1 0042214-02082018162437.pdf 10797916.pdf 2018-08-02T16:24:37.0600000 Output 23077404 application/pdf E-Thesis true 2018-08-02T00:00:00.0000000 false
title On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling.
spellingShingle On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling.
LEI JIANG
title_short On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling.
title_full On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling.
title_fullStr On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling.
title_full_unstemmed On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling.
title_sort On human gut microbial ecosystem: In vitro experiment, in vivo study and mathematical modelling.
author_id_str_mv 83091f58166b25607411a755706bd449
author_id_fullname_str_mv 83091f58166b25607411a755706bd449_***_LEI JIANG
author LEI JIANG
author2 LEI JIANG
format E-Thesis
publishDate 2013
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 1
active_str 0
description The human gut microbiota is considered to be a highly specialized organ providing nourishment, regulating epithelial cell development, modulating innate immune responses and colonization resistances, and it significantly impacts human health and disease. Dispite of being extensively studied for several decades, the functionality of the microbiota colonization in the human gastrointestinal tract and the mechanisms of the interactions between the host and bacteria are still poorly understood. This research follows a novel and unique approach, which combines the complementary strengths of in vitro experiment, in vivo study and mathematical modelling. The work undertaken has three emphases: 1) probiotic strains and their impact on human health; 2) the development of gut microbiota in infants; 3) quantification of human gut microbial ecosystem at both the species level and the system level. In the first part of this research, a versatile anaerobic continuous culture platform was implemented following a novel and unique design, which allows easy and continuous sampling and monitoring of microbial growth. A number of carefully planned in vitro experiments have been conducted to investigate the growth and competition of probiotic strains under different culture conditions. These in vitro experiments improve the understanding for the growth behaviour of the specific probiotic strains. The second part of this project analyzed 50 faecal samples collected from 9 healthy infants with administration of probiotic strains and placebo. The analysis is based on the 454-pyrosequencing technology, which reveals the complete profiles of gut microbiota in these infants and confirmed the modulation effect of the specific probiotic strains. The last part of this research focused on the development of mathematical and computational models of human gut microbial ecosystem. The outcome from this part of the research includes: a) a new bacterial growth model that overcomes the parodox of competitative exclusion caused by previous models; b) a versatile computational framework to simulate in vitro fermentation experiments; and c) a comprehensive mathematical model for human gut and gut microbiota that is the first model for its nature.
published_date 2013-12-31T03:52:31Z
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score 11.012678

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