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Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor. / Bhavesh V Mistry

Swansea University Author: Bhavesh V Mistry

Abstract

"The rodA and ftsW genes encode polytopic membrane proteins that are essential for bacterial cell elongation and division, respectively. These genes are highly conserved among bacteria with a peptidoglycan cell wall and belong to the SEDS ("shape, elongation, division, and sporulation"...

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Published: 2008
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa42448
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spelling 2018-08-29T14:59:51.7994797 v2 42448 2018-08-02 Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor. bda3bfc796c5d86a6b19592d5d6b1c08 NULL Bhavesh V Mistry Bhavesh V Mistry true true 2018-08-02 "The rodA and ftsW genes encode polytopic membrane proteins that are essential for bacterial cell elongation and division, respectively. These genes are highly conserved among bacteria with a peptidoglycan cell wall and belong to the SEDS ("shape, elongation, division, and sporulation") gene family. Each SEDS gene is invariably linked with a cognate class B high-molecular weight penicillin-binding protein (HMW PBP) gene. Four such pairs of genes are found in the genome of filamentous differentiating Streptomyces coelicolor. This study focused on characterization of four SEDS genes [SC02085 (ftsW), SC02607 (sfr), SC03846 (rodA) and SC05302 (rodA2)] and SC02090 (ftsi) and SC02608 ipbp2) [cognate HMW PBP genes ftsWand sfr, respectively]. Computational analysis of each SEDS gene locus revealed that each gene is a part of specific gene cluster. Construction of disruption mutants of each SEDS gene revealed ftsW, sfr and rodA2 are dispensable for growth and survival of S. coelioclor, whereas rodA is essential. Mutation of sfr or rodA2 did not cause gross changes to growth and septation of the organism. However, the mutation in sfr made the spores susceptible to heat, SDS and cell wall specific antibiotics. Similar effects were observed in the php2 (cognate HMW PBP gene of sfr) disrupted mutant. The susceptibility of the spores of sfr and php2 mutants to such a physical and chemical stress implies an important role of these genes in spore wall synthesis. Disruption of either or the cognate ftsI gene blocked the formation of sporulation septation in aerial hyphae. The inability of spiral polymers of FtsZ to reorganize into rings in aerial hyphae of these mutants indicates an early pivotal role of an FtsW-FtsI complex in cell division. Mutants of ftsQ were also unable to sporulate and the cytological analysis of this mutant showed that it was blocked at a later stage in cell division, during septum closure. Analysis of FtsZ distribution in ftsQ mutant aerial hyphae revealed that concerted assembly of the complete divisome was not required for Z ring stabilization in this mutant. Complete cross-wall formation in the vegetative hyphae of all three fts mutants imply that the typical bacterial divisome functions specifically during non-essential sporulation septation. Thus, it provides a unique opportunity to investigate the function and dependencies of individual components of the divisome in vivo." E-Thesis Genetics.;Molecular biology. 31 12 2008 2008-12-31 COLLEGE NANME Swansea University Medical School COLLEGE CODE Swansea University Doctoral Ph.D 2018-08-29T14:59:51.7994797 2018-08-02T16:24:29.2909995 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Bhavesh V Mistry NULL 1 0042448-02082018162455.pdf 10798156.pdf 2018-08-02T16:24:55.2170000 Output 34403572 application/pdf E-Thesis true 2018-08-02T16:24:55.2170000 false
title Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor.
spellingShingle Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor.
Bhavesh V Mistry
title_short Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor.
title_full Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor.
title_fullStr Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor.
title_full_unstemmed Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor.
title_sort Analysis of SEDS proteins and their cognate PBPs in Streptomyces coelicolor.
author_id_str_mv bda3bfc796c5d86a6b19592d5d6b1c08
author_id_fullname_str_mv bda3bfc796c5d86a6b19592d5d6b1c08_***_Bhavesh V Mistry
author Bhavesh V Mistry
author2 Bhavesh V Mistry
format E-Thesis
publishDate 2008
institution Swansea University
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description "The rodA and ftsW genes encode polytopic membrane proteins that are essential for bacterial cell elongation and division, respectively. These genes are highly conserved among bacteria with a peptidoglycan cell wall and belong to the SEDS ("shape, elongation, division, and sporulation") gene family. Each SEDS gene is invariably linked with a cognate class B high-molecular weight penicillin-binding protein (HMW PBP) gene. Four such pairs of genes are found in the genome of filamentous differentiating Streptomyces coelicolor. This study focused on characterization of four SEDS genes [SC02085 (ftsW), SC02607 (sfr), SC03846 (rodA) and SC05302 (rodA2)] and SC02090 (ftsi) and SC02608 ipbp2) [cognate HMW PBP genes ftsWand sfr, respectively]. Computational analysis of each SEDS gene locus revealed that each gene is a part of specific gene cluster. Construction of disruption mutants of each SEDS gene revealed ftsW, sfr and rodA2 are dispensable for growth and survival of S. coelioclor, whereas rodA is essential. Mutation of sfr or rodA2 did not cause gross changes to growth and septation of the organism. However, the mutation in sfr made the spores susceptible to heat, SDS and cell wall specific antibiotics. Similar effects were observed in the php2 (cognate HMW PBP gene of sfr) disrupted mutant. The susceptibility of the spores of sfr and php2 mutants to such a physical and chemical stress implies an important role of these genes in spore wall synthesis. Disruption of either or the cognate ftsI gene blocked the formation of sporulation septation in aerial hyphae. The inability of spiral polymers of FtsZ to reorganize into rings in aerial hyphae of these mutants indicates an early pivotal role of an FtsW-FtsI complex in cell division. Mutants of ftsQ were also unable to sporulate and the cytological analysis of this mutant showed that it was blocked at a later stage in cell division, during septum closure. Analysis of FtsZ distribution in ftsQ mutant aerial hyphae revealed that concerted assembly of the complete divisome was not required for Z ring stabilization in this mutant. Complete cross-wall formation in the vegetative hyphae of all three fts mutants imply that the typical bacterial divisome functions specifically during non-essential sporulation septation. Thus, it provides a unique opportunity to investigate the function and dependencies of individual components of the divisome in vivo."
published_date 2008-12-31T03:52:59Z
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score 11.036706

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