No Cover Image

E-Thesis 185 views 179 downloads

Investigation and utilization of lactic acid bacteria for cider maturation processes. / Daosheng Zhang

Swansea University Author: Daosheng Zhang

Abstract

Investigation and utilization of Lactic Acid Bacteria for cider maturation processes Cider maturation involves a number of transformations mediated by Lactic Acid Bacteria (LAB). A comparison of two heterofermentative lactic acid bacteria (Oenococcus oeni 11648 and Lactobacillus brevis X2), capable...

Full description

Published: 2004
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa42642
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2018-08-02T18:55:12Z
last_indexed 2018-08-03T10:10:41Z
id cronfa42642
recordtype RisThesis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2018-08-02T16:24:29.9461990</datestamp><bib-version>v2</bib-version><id>42642</id><entry>2018-08-02</entry><title>Investigation and utilization of lactic acid bacteria for cider maturation processes.</title><swanseaauthors><author><sid>e50e5603a26763b193235b6b1e78b022</sid><ORCID>NULL</ORCID><firstname>Daosheng</firstname><surname>Zhang</surname><name>Daosheng Zhang</name><active>true</active><ethesisStudent>true</ethesisStudent></author></swanseaauthors><date>2018-08-02</date><abstract>Investigation and utilization of Lactic Acid Bacteria for cider maturation processes Cider maturation involves a number of transformations mediated by Lactic Acid Bacteria (LAB). A comparison of two heterofermentative lactic acid bacteria (Oenococcus oeni 11648 and Lactobacillus brevis X2), capable of malolactic fermentation, was made to assess the relative merits of these two organisms in cider maturation processes. Initial work studied LAB propagation on safe (free of animal extracts) simple media. Optimum growth conditions were determined. The two strains were shown to be highly adapted to the cider environment being able to tolerate ethanol, up to 10% (v/v). O. oeni was shown to be more tolerant to ethanol and low pH condition compared to L. brevis. The growth of both strains was significantly stimulated by low levels of glucose (~ 1mM) in the presence of organic acids (5g/l malate) such that the specific growth rate and cell yield were stimulated by 167% and 260% respectively. The growth of the organisms was studied in batch, continuous and membrane bioreactor (MBR) systems with pH control. The highest maximum specific growth rates of O. oeni with 0.066h-1 was obtained at pH 4.5 when the mixture of 5g/l glucose-5g/l fructose was added to the culture medium, while L. brevis with 0.110h-1 was obtained at pH 5.5 in the 10g/1 glucose medium. Both strains were grown in continuous cultures at a variety of dilution rates and the precise growth parameters were determined and these data show that L. brevis gave higher biomass yield (Yx/smax), faster volumetric cell productivity (Qxmax) higher ATP yield (YATPmax) than O. oeni. In the MBR culture system, the growth of two LAB showed that high cell concentrations were possible, O. oeni cultures to 12 g/1 of dry weight, this is over 32 times higher than batch (0.357 g/1) and continuous culture (0.37 g/1). The volumetric productivities of the three systems were 7.9 mg/l/h, 17.6 mg/l/h and 180mg/l/h in batch, continuous culture and MBR respectively. Similar results were obtained for the growth of L. brevis. Having produced high cell concentrations of O. oeni and L. brevis in the MBR, the system was employed for rapid, continuous malolactic transformation of synthetic green cider medium. The levels of malate removed were a function of the residence time and the alcohol concentration. Starting with 2 g/1 malate, over 85% could be removed using a 6 hour residence time and compared very favourably with conventional batch fermentations that take typically 20 - 40 days. The MBR system was run successfully for a period of over 9 days and with correct management could be run for much longer periods. In these conditions, O. oeni showed greater stability and viability than L. brevis in cider environment as it is more alcohol and acid tolerant. These studies provide a basis for developing strategies for rapid maturation of cider using an MBR system. Future work for further optimization of the MBR operation with real cider is discussed. The economic viability of MBR in cider maturation needs to be assessed.</abstract><type>E-Thesis</type><journal/><journalNumber></journalNumber><paginationStart/><paginationEnd/><publisher/><placeOfPublication/><isbnPrint/><issnPrint/><issnElectronic/><keywords>Bioengineering.;Agricultural engineering.;Food science.</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2004</publishedYear><publishedDate>2004-12-31</publishedDate><doi/><url/><notes/><college>COLLEGE NANME</college><department>Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><degreelevel>Doctoral</degreelevel><degreename>Ph.D</degreename><apcterm/><lastEdited>2018-08-02T16:24:29.9461990</lastEdited><Created>2018-08-02T16:24:29.9461990</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Uncategorised</level></path><authors><author><firstname>Daosheng</firstname><surname>Zhang</surname><orcid>NULL</orcid><order>1</order></author></authors><documents><document><filename>0042642-02082018162510.pdf</filename><originalFilename>10805418.pdf</originalFilename><uploaded>2018-08-02T16:25:10.5230000</uploaded><type>Output</type><contentLength>11384075</contentLength><contentType>application/pdf</contentType><version>E-Thesis</version><cronfaStatus>true</cronfaStatus><embargoDate>2018-08-02T16:25:10.5230000</embargoDate><copyrightCorrect>false</copyrightCorrect></document></documents><OutputDurs/></rfc1807>
spelling 2018-08-02T16:24:29.9461990 v2 42642 2018-08-02 Investigation and utilization of lactic acid bacteria for cider maturation processes. e50e5603a26763b193235b6b1e78b022 NULL Daosheng Zhang Daosheng Zhang true true 2018-08-02 Investigation and utilization of Lactic Acid Bacteria for cider maturation processes Cider maturation involves a number of transformations mediated by Lactic Acid Bacteria (LAB). A comparison of two heterofermentative lactic acid bacteria (Oenococcus oeni 11648 and Lactobacillus brevis X2), capable of malolactic fermentation, was made to assess the relative merits of these two organisms in cider maturation processes. Initial work studied LAB propagation on safe (free of animal extracts) simple media. Optimum growth conditions were determined. The two strains were shown to be highly adapted to the cider environment being able to tolerate ethanol, up to 10% (v/v). O. oeni was shown to be more tolerant to ethanol and low pH condition compared to L. brevis. The growth of both strains was significantly stimulated by low levels of glucose (~ 1mM) in the presence of organic acids (5g/l malate) such that the specific growth rate and cell yield were stimulated by 167% and 260% respectively. The growth of the organisms was studied in batch, continuous and membrane bioreactor (MBR) systems with pH control. The highest maximum specific growth rates of O. oeni with 0.066h-1 was obtained at pH 4.5 when the mixture of 5g/l glucose-5g/l fructose was added to the culture medium, while L. brevis with 0.110h-1 was obtained at pH 5.5 in the 10g/1 glucose medium. Both strains were grown in continuous cultures at a variety of dilution rates and the precise growth parameters were determined and these data show that L. brevis gave higher biomass yield (Yx/smax), faster volumetric cell productivity (Qxmax) higher ATP yield (YATPmax) than O. oeni. In the MBR culture system, the growth of two LAB showed that high cell concentrations were possible, O. oeni cultures to 12 g/1 of dry weight, this is over 32 times higher than batch (0.357 g/1) and continuous culture (0.37 g/1). The volumetric productivities of the three systems were 7.9 mg/l/h, 17.6 mg/l/h and 180mg/l/h in batch, continuous culture and MBR respectively. Similar results were obtained for the growth of L. brevis. Having produced high cell concentrations of O. oeni and L. brevis in the MBR, the system was employed for rapid, continuous malolactic transformation of synthetic green cider medium. The levels of malate removed were a function of the residence time and the alcohol concentration. Starting with 2 g/1 malate, over 85% could be removed using a 6 hour residence time and compared very favourably with conventional batch fermentations that take typically 20 - 40 days. The MBR system was run successfully for a period of over 9 days and with correct management could be run for much longer periods. In these conditions, O. oeni showed greater stability and viability than L. brevis in cider environment as it is more alcohol and acid tolerant. These studies provide a basis for developing strategies for rapid maturation of cider using an MBR system. Future work for further optimization of the MBR operation with real cider is discussed. The economic viability of MBR in cider maturation needs to be assessed. E-Thesis Bioengineering.;Agricultural engineering.;Food science. 31 12 2004 2004-12-31 COLLEGE NANME Engineering COLLEGE CODE Swansea University Doctoral Ph.D 2018-08-02T16:24:29.9461990 2018-08-02T16:24:29.9461990 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Daosheng Zhang NULL 1 0042642-02082018162510.pdf 10805418.pdf 2018-08-02T16:25:10.5230000 Output 11384075 application/pdf E-Thesis true 2018-08-02T16:25:10.5230000 false
title Investigation and utilization of lactic acid bacteria for cider maturation processes.
spellingShingle Investigation and utilization of lactic acid bacteria for cider maturation processes.
Daosheng Zhang
title_short Investigation and utilization of lactic acid bacteria for cider maturation processes.
title_full Investigation and utilization of lactic acid bacteria for cider maturation processes.
title_fullStr Investigation and utilization of lactic acid bacteria for cider maturation processes.
title_full_unstemmed Investigation and utilization of lactic acid bacteria for cider maturation processes.
title_sort Investigation and utilization of lactic acid bacteria for cider maturation processes.
author_id_str_mv e50e5603a26763b193235b6b1e78b022
author_id_fullname_str_mv e50e5603a26763b193235b6b1e78b022_***_Daosheng Zhang
author Daosheng Zhang
author2 Daosheng Zhang
format E-Thesis
publishDate 2004
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 1
active_str 0
description Investigation and utilization of Lactic Acid Bacteria for cider maturation processes Cider maturation involves a number of transformations mediated by Lactic Acid Bacteria (LAB). A comparison of two heterofermentative lactic acid bacteria (Oenococcus oeni 11648 and Lactobacillus brevis X2), capable of malolactic fermentation, was made to assess the relative merits of these two organisms in cider maturation processes. Initial work studied LAB propagation on safe (free of animal extracts) simple media. Optimum growth conditions were determined. The two strains were shown to be highly adapted to the cider environment being able to tolerate ethanol, up to 10% (v/v). O. oeni was shown to be more tolerant to ethanol and low pH condition compared to L. brevis. The growth of both strains was significantly stimulated by low levels of glucose (~ 1mM) in the presence of organic acids (5g/l malate) such that the specific growth rate and cell yield were stimulated by 167% and 260% respectively. The growth of the organisms was studied in batch, continuous and membrane bioreactor (MBR) systems with pH control. The highest maximum specific growth rates of O. oeni with 0.066h-1 was obtained at pH 4.5 when the mixture of 5g/l glucose-5g/l fructose was added to the culture medium, while L. brevis with 0.110h-1 was obtained at pH 5.5 in the 10g/1 glucose medium. Both strains were grown in continuous cultures at a variety of dilution rates and the precise growth parameters were determined and these data show that L. brevis gave higher biomass yield (Yx/smax), faster volumetric cell productivity (Qxmax) higher ATP yield (YATPmax) than O. oeni. In the MBR culture system, the growth of two LAB showed that high cell concentrations were possible, O. oeni cultures to 12 g/1 of dry weight, this is over 32 times higher than batch (0.357 g/1) and continuous culture (0.37 g/1). The volumetric productivities of the three systems were 7.9 mg/l/h, 17.6 mg/l/h and 180mg/l/h in batch, continuous culture and MBR respectively. Similar results were obtained for the growth of L. brevis. Having produced high cell concentrations of O. oeni and L. brevis in the MBR, the system was employed for rapid, continuous malolactic transformation of synthetic green cider medium. The levels of malate removed were a function of the residence time and the alcohol concentration. Starting with 2 g/1 malate, over 85% could be removed using a 6 hour residence time and compared very favourably with conventional batch fermentations that take typically 20 - 40 days. The MBR system was run successfully for a period of over 9 days and with correct management could be run for much longer periods. In these conditions, O. oeni showed greater stability and viability than L. brevis in cider environment as it is more alcohol and acid tolerant. These studies provide a basis for developing strategies for rapid maturation of cider using an MBR system. Future work for further optimization of the MBR operation with real cider is discussed. The economic viability of MBR in cider maturation needs to be assessed.
published_date 2004-12-31T03:53:22Z
_version_ 1763752649384001536
score 11.012857

Similar Items