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Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
Advanced Materials, Volume: 30, Issue: 40, Start page: 1802732
Swansea University Authors: Huw Summers , Justyna Piasecka
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DOI (Published version): 10.1002/adma.201802732
Abstract
Hard corona (HC) protein, i.e., the environmental proteins of the biological medium that are bound to a nanosurface, is known to affect the biological fate of a nanomedicine. Due to the size, curvature, and specific surface area (SSA) 3‐factor interactions inherited in the traditional 3D nanoparticl...
Published in: | Advanced Materials |
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ISSN: | 0935-9648 |
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Wiley
2018
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URI: | https://cronfa.swan.ac.uk/Record/cronfa43705 |
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<?xml version="1.0"?><rfc1807><datestamp>2020-07-25T18:36:36.3608577</datestamp><bib-version>v2</bib-version><id>43705</id><entry>2018-09-06</entry><title>Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines</title><swanseaauthors><author><sid>a61c15e220837ebfa52648c143769427</sid><ORCID>0000-0002-0898-5612</ORCID><firstname>Huw</firstname><surname>Summers</surname><name>Huw Summers</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>929d37d0bcff9a9c2d7dd74fe120b22e</sid><ORCID/><firstname>Justyna</firstname><surname>Piasecka</surname><name>Justyna Piasecka</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2018-09-06</date><deptcode>MEDE</deptcode><abstract>Hard corona (HC) protein, i.e., the environmental proteins of the biological medium that are bound to a nanosurface, is known to affect the biological fate of a nanomedicine. Due to the size, curvature, and specific surface area (SSA) 3‐factor interactions inherited in the traditional 3D nanoparticle, HC‐dependent bio–nano interactions are often poorly probed and interpreted. Here, the first HC‐by‐design case study in 2D is demonstrated that sequentially and linearly changes the HC quantity using functionalized graphene oxide (GO) nanosheets. The HC quantity and HC quality are analyzed using NanoDrop and label‐free liquid chromatography–mass spectrometry (LC‐MS) followed by principal component analysis (PCA). Cellular responses (uptake and cytotoxicity in J774 cell model) are compared using imaging cytometry and the modified lactate dehydrogenase assays, respectively. Cellular uptake linearly and solely correlates with HC quantity (R2 = 0.99634). The nanotoxicity, analyzed by retrospective design of experiment (DoE), is found to be dependent on the nanomaterial uptake (primary), HC composition (secondary), and nanomaterial exposure dose (tertiary). This unique 2D design eliminates the size–curvature–SSA multifactor interactions and can serve as a reliable screening platform to uncover HC‐dependent bio–nano interactions to enable the next‐generation quality‐by‐design (QbD) nanomedicines for better clinical translation.</abstract><type>Journal Article</type><journal>Advanced Materials</journal><volume>30</volume><journalNumber>40</journalNumber><paginationStart>1802732</paginationStart><publisher>Wiley</publisher><issnPrint>0935-9648</issnPrint><keywords/><publishedDay>1</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2018</publishedYear><publishedDate>2018-10-01</publishedDate><doi>10.1002/adma.201802732</doi><url/><notes>This inter-disciplinary paper addresses the critical issue in nano-biotechnology of the protein corona that surrounds nanoparticles. It presents the first engineered approach to control the corona using graphene materials. The importance of the Swansea contribution was in the provision of detailed, quantitative analysis of nanoparticle decoration of cells using imaging cytometry. This was crucial in allowing interpretation of dimensionless PCA scores into meaningful biological function using correlation studies, based on the imaging technology.</notes><college>COLLEGE NANME</college><department>Biomedical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDE</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-07-25T18:36:36.3608577</lastEdited><Created>2018-09-06T09:59:25.8635496</Created><authors><author><firstname>Kuo-Ching</firstname><surname>Mei</surname><order>1</order></author><author><firstname>Artur</firstname><surname>Ghazaryan</surname><order>2</order></author><author><firstname>Er Zhen</firstname><surname>Teoh</surname><order>3</order></author><author><firstname>Huw</firstname><surname>Summers</surname><orcid>0000-0002-0898-5612</orcid><order>4</order></author><author><firstname>Yueting</firstname><surname>Li</surname><order>5</order></author><author><firstname>Belén</firstname><surname>Ballesteros</surname><order>6</order></author><author><firstname>Justyna</firstname><surname>Piasecka</surname><orcid/><order>7</order></author><author><firstname>Adam</firstname><surname>Walters</surname><order>8</order></author><author><firstname>Robert C.</firstname><surname>Hider</surname><order>9</order></author><author><firstname>Volker</firstname><surname>Mailänder</surname><order>10</order></author><author><firstname>Khuloud T.</firstname><surname>Al-Jamal</surname><order>11</order></author></authors><documents><document><filename>0043705-06092018100506.pdf</filename><originalFilename>mei2018(2).pdf</originalFilename><uploaded>2018-09-06T10:05:06.3230000</uploaded><type>Output</type><contentLength>2554106</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>Released under the terms of a Creative Commons Attribution License (CC-BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807> |
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2020-07-25T18:36:36.3608577 v2 43705 2018-09-06 Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines a61c15e220837ebfa52648c143769427 0000-0002-0898-5612 Huw Summers Huw Summers true false 929d37d0bcff9a9c2d7dd74fe120b22e Justyna Piasecka Justyna Piasecka true false 2018-09-06 MEDE Hard corona (HC) protein, i.e., the environmental proteins of the biological medium that are bound to a nanosurface, is known to affect the biological fate of a nanomedicine. Due to the size, curvature, and specific surface area (SSA) 3‐factor interactions inherited in the traditional 3D nanoparticle, HC‐dependent bio–nano interactions are often poorly probed and interpreted. Here, the first HC‐by‐design case study in 2D is demonstrated that sequentially and linearly changes the HC quantity using functionalized graphene oxide (GO) nanosheets. The HC quantity and HC quality are analyzed using NanoDrop and label‐free liquid chromatography–mass spectrometry (LC‐MS) followed by principal component analysis (PCA). Cellular responses (uptake and cytotoxicity in J774 cell model) are compared using imaging cytometry and the modified lactate dehydrogenase assays, respectively. Cellular uptake linearly and solely correlates with HC quantity (R2 = 0.99634). The nanotoxicity, analyzed by retrospective design of experiment (DoE), is found to be dependent on the nanomaterial uptake (primary), HC composition (secondary), and nanomaterial exposure dose (tertiary). This unique 2D design eliminates the size–curvature–SSA multifactor interactions and can serve as a reliable screening platform to uncover HC‐dependent bio–nano interactions to enable the next‐generation quality‐by‐design (QbD) nanomedicines for better clinical translation. Journal Article Advanced Materials 30 40 1802732 Wiley 0935-9648 1 10 2018 2018-10-01 10.1002/adma.201802732 This inter-disciplinary paper addresses the critical issue in nano-biotechnology of the protein corona that surrounds nanoparticles. It presents the first engineered approach to control the corona using graphene materials. The importance of the Swansea contribution was in the provision of detailed, quantitative analysis of nanoparticle decoration of cells using imaging cytometry. This was crucial in allowing interpretation of dimensionless PCA scores into meaningful biological function using correlation studies, based on the imaging technology. COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2020-07-25T18:36:36.3608577 2018-09-06T09:59:25.8635496 Kuo-Ching Mei 1 Artur Ghazaryan 2 Er Zhen Teoh 3 Huw Summers 0000-0002-0898-5612 4 Yueting Li 5 Belén Ballesteros 6 Justyna Piasecka 7 Adam Walters 8 Robert C. Hider 9 Volker Mailänder 10 Khuloud T. Al-Jamal 11 0043705-06092018100506.pdf mei2018(2).pdf 2018-09-06T10:05:06.3230000 Output 2554106 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution License (CC-BY). true eng http://creativecommons.org/licenses/by/4.0/ |
title |
Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines |
spellingShingle |
Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines Huw Summers Justyna Piasecka |
title_short |
Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines |
title_full |
Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines |
title_fullStr |
Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines |
title_full_unstemmed |
Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines |
title_sort |
Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines |
author_id_str_mv |
a61c15e220837ebfa52648c143769427 929d37d0bcff9a9c2d7dd74fe120b22e |
author_id_fullname_str_mv |
a61c15e220837ebfa52648c143769427_***_Huw Summers 929d37d0bcff9a9c2d7dd74fe120b22e_***_Justyna Piasecka |
author |
Huw Summers Justyna Piasecka |
author2 |
Kuo-Ching Mei Artur Ghazaryan Er Zhen Teoh Huw Summers Yueting Li Belén Ballesteros Justyna Piasecka Adam Walters Robert C. Hider Volker Mailänder Khuloud T. Al-Jamal |
format |
Journal article |
container_title |
Advanced Materials |
container_volume |
30 |
container_issue |
40 |
container_start_page |
1802732 |
publishDate |
2018 |
institution |
Swansea University |
issn |
0935-9648 |
doi_str_mv |
10.1002/adma.201802732 |
publisher |
Wiley |
document_store_str |
1 |
active_str |
0 |
description |
Hard corona (HC) protein, i.e., the environmental proteins of the biological medium that are bound to a nanosurface, is known to affect the biological fate of a nanomedicine. Due to the size, curvature, and specific surface area (SSA) 3‐factor interactions inherited in the traditional 3D nanoparticle, HC‐dependent bio–nano interactions are often poorly probed and interpreted. Here, the first HC‐by‐design case study in 2D is demonstrated that sequentially and linearly changes the HC quantity using functionalized graphene oxide (GO) nanosheets. The HC quantity and HC quality are analyzed using NanoDrop and label‐free liquid chromatography–mass spectrometry (LC‐MS) followed by principal component analysis (PCA). Cellular responses (uptake and cytotoxicity in J774 cell model) are compared using imaging cytometry and the modified lactate dehydrogenase assays, respectively. Cellular uptake linearly and solely correlates with HC quantity (R2 = 0.99634). The nanotoxicity, analyzed by retrospective design of experiment (DoE), is found to be dependent on the nanomaterial uptake (primary), HC composition (secondary), and nanomaterial exposure dose (tertiary). This unique 2D design eliminates the size–curvature–SSA multifactor interactions and can serve as a reliable screening platform to uncover HC‐dependent bio–nano interactions to enable the next‐generation quality‐by‐design (QbD) nanomedicines for better clinical translation. |
published_date |
2018-10-01T03:55:01Z |
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1763752753397497856 |
score |
11.016235 |