No Cover Image

Journal article 224 views 30 downloads

The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid / Christopher J. Coates; Jenson Lim; Katie Harman; Andrew F. Rowley; David J. Griffiths; Helena Emery; Will Layton

Cell Biology and Toxicology, Volume: 35, Pages: 219 - 232

Swansea University Author: Coates, Christopher

  • 44678.pdf

    PDF | Version of Record

    Released under the terms of a Creative Commons Attribution 4.0 International License (CC-BY).

    Download (1.54MB)

Abstract

The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiol...

Full description

Published in: Cell Biology and Toxicology
ISSN: 0742-2091 1573-6822
Published: Springer 2019
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa44678
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiologically relevant doses of okadaic acid by direct injection into the haemocoel (body cavity) and/or gavage (force-feeding). We monitored larval survival and employed a range of cellular and biochemical assays to assess the potential harmful effects of okadaic acid. Okadaic acid at concentrations >75 ng/larva (>242 µg/kg) led to significant reductions in larval survival (>65%) and circulating haemocyte (blood cell) numbers (>50%) within 24 h post-inoculation. In the haemolymph, okadaic acid reduced haemocyte viability and increased phenoloxidase activities. In the midgut, okadaic acid induced oxidative damage as determined by increases in superoxide dismutase activity and levels of malondialdehyde (i.e., lipid peroxidation). Our observations of insect larvae correspond broadly to data published using rodent models of shellfish poisoning toxidrome, including complementary LD50 values; 206–242 μg/kg in mice, ~239 μg/kg in G. mellonella. These data support the use of this insect as a surrogate model for the investigation of marine toxins, which offers distinct ethical and financial incentives.
Keywords: Haemocytes; Innate Immunity; Oxidative stress; Phenoloxidase; Shellfish poisoning syndrome; immunotoxicology
College: College of Science
Start Page: 219
End Page: 232