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The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
Cell Biology and Toxicology, Volume: 35, Pages: 219 - 232
Swansea University Authors: Christopher Coates, Andrew Rowley
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DOI (Published version): 10.1007/s10565-018-09448-2
Abstract
The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiol...
Published in: | Cell Biology and Toxicology |
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ISSN: | 0742-2091 1573-6822 |
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Springer
2019
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URI: | https://cronfa.swan.ac.uk/Record/cronfa44678 |
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2020-09-21T17:25:28.2732684 v2 44678 2018-10-02 The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid af160934b75bea5b8ba83d68b3d1a003 Christopher Coates Christopher Coates true false e98124f6e62b9592786899d7059e3a79 Andrew Rowley Andrew Rowley true false 2018-10-02 The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiologically relevant doses of okadaic acid by direct injection into the haemocoel (body cavity) and/or gavage (force-feeding). We monitored larval survival and employed a range of cellular and biochemical assays to assess the potential harmful effects of okadaic acid. Okadaic acid at concentrations >75 ng/larva (>242 µg/kg) led to significant reductions in larval survival (>65%) and circulating haemocyte (blood cell) numbers (>50%) within 24 h post-inoculation. In the haemolymph, okadaic acid reduced haemocyte viability and increased phenoloxidase activities. In the midgut, okadaic acid induced oxidative damage as determined by increases in superoxide dismutase activity and levels of malondialdehyde (i.e., lipid peroxidation). Our observations of insect larvae correspond broadly to data published using rodent models of shellfish poisoning toxidrome, including complementary LD50 values; 206–242 μg/kg in mice, ~239 μg/kg in G. mellonella. These data support the use of this insect as a surrogate model for the investigation of marine toxins, which offers distinct ethical and financial incentives. Journal Article Cell Biology and Toxicology 35 219 232 Springer 0742-2091 1573-6822 Haemocytes; Innate Immunity; Oxidative stress; Phenoloxidase; Shellfish poisoning syndrome; immunotoxicology 10 6 2019 2019-06-10 10.1007/s10565-018-09448-2 COLLEGE NANME COLLEGE CODE Swansea University 2020-09-21T17:25:28.2732684 2018-10-02T13:51:32.1861794 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Christopher Coates 1 Jenson Lim 2 Katie Harman 3 Andrew Rowley 4 David J. Griffiths 5 Helena Emery 6 Will Layton 7 0044678-13112018154622.pdf 44678.pdf 2018-11-13T15:46:22.9600000 Output 1684448 application/pdf Version of Record true 2018-11-12T00:00:00.0000000 Released under the terms of a Creative Commons Attribution 4.0 International License (CC-BY). true eng |
title |
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid |
spellingShingle |
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid Christopher Coates Andrew Rowley |
title_short |
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid |
title_full |
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid |
title_fullStr |
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid |
title_full_unstemmed |
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid |
title_sort |
The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid |
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af160934b75bea5b8ba83d68b3d1a003 e98124f6e62b9592786899d7059e3a79 |
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af160934b75bea5b8ba83d68b3d1a003_***_Christopher Coates e98124f6e62b9592786899d7059e3a79_***_Andrew Rowley |
author |
Christopher Coates Andrew Rowley |
author2 |
Christopher Coates Jenson Lim Katie Harman Andrew Rowley David J. Griffiths Helena Emery Will Layton |
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Cell Biology and Toxicology |
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35 |
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219 |
publishDate |
2019 |
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Swansea University |
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0742-2091 1573-6822 |
doi_str_mv |
10.1007/s10565-018-09448-2 |
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Springer |
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Faculty of Science and Engineering |
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School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences |
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description |
The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiologically relevant doses of okadaic acid by direct injection into the haemocoel (body cavity) and/or gavage (force-feeding). We monitored larval survival and employed a range of cellular and biochemical assays to assess the potential harmful effects of okadaic acid. Okadaic acid at concentrations >75 ng/larva (>242 µg/kg) led to significant reductions in larval survival (>65%) and circulating haemocyte (blood cell) numbers (>50%) within 24 h post-inoculation. In the haemolymph, okadaic acid reduced haemocyte viability and increased phenoloxidase activities. In the midgut, okadaic acid induced oxidative damage as determined by increases in superoxide dismutase activity and levels of malondialdehyde (i.e., lipid peroxidation). Our observations of insect larvae correspond broadly to data published using rodent models of shellfish poisoning toxidrome, including complementary LD50 values; 206–242 μg/kg in mice, ~239 μg/kg in G. mellonella. These data support the use of this insect as a surrogate model for the investigation of marine toxins, which offers distinct ethical and financial incentives. |
published_date |
2019-06-10T03:56:00Z |
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1763752815624192000 |
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10.999547 |