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The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid

Christopher Coates, Jenson Lim, Katie Harman, Andrew Rowley, David J. Griffiths, Helena Emery, Will Layton

Cell Biology and Toxicology, Volume: 35, Pages: 219 - 232

Swansea University Authors: Christopher Coates, Andrew Rowley

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Abstract

The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiol...

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Published in: Cell Biology and Toxicology
ISSN: 0742-2091 1573-6822
Published: Springer 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa44678
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spelling 2020-09-21T17:25:28.2732684 v2 44678 2018-10-02 The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid af160934b75bea5b8ba83d68b3d1a003 Christopher Coates Christopher Coates true false e98124f6e62b9592786899d7059e3a79 Andrew Rowley Andrew Rowley true false 2018-10-02 The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiologically relevant doses of okadaic acid by direct injection into the haemocoel (body cavity) and/or gavage (force-feeding). We monitored larval survival and employed a range of cellular and biochemical assays to assess the potential harmful effects of okadaic acid. Okadaic acid at concentrations >75 ng/larva (>242 µg/kg) led to significant reductions in larval survival (>65%) and circulating haemocyte (blood cell) numbers (>50%) within 24 h post-inoculation. In the haemolymph, okadaic acid reduced haemocyte viability and increased phenoloxidase activities. In the midgut, okadaic acid induced oxidative damage as determined by increases in superoxide dismutase activity and levels of malondialdehyde (i.e., lipid peroxidation). Our observations of insect larvae correspond broadly to data published using rodent models of shellfish poisoning toxidrome, including complementary LD50 values; 206–242 μg/kg in mice, ~239 μg/kg in G. mellonella. These data support the use of this insect as a surrogate model for the investigation of marine toxins, which offers distinct ethical and financial incentives. Journal Article Cell Biology and Toxicology 35 219 232 Springer 0742-2091 1573-6822 Haemocytes; Innate Immunity; Oxidative stress; Phenoloxidase; Shellfish poisoning syndrome; immunotoxicology 10 6 2019 2019-06-10 10.1007/s10565-018-09448-2 COLLEGE NANME COLLEGE CODE Swansea University 2020-09-21T17:25:28.2732684 2018-10-02T13:51:32.1861794 College of Science Biosciences Christopher Coates 1 Jenson Lim 2 Katie Harman 3 Andrew Rowley 4 David J. Griffiths 5 Helena Emery 6 Will Layton 7 0044678-13112018154622.pdf 44678.pdf 2018-11-13T15:46:22.9600000 Output 1684448 application/pdf Version of Record true 2018-11-12T00:00:00.0000000 Released under the terms of a Creative Commons Attribution 4.0 International License (CC-BY). true eng
title The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
spellingShingle The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
Christopher Coates
Andrew Rowley
title_short The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
title_full The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
title_fullStr The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
title_full_unstemmed The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
title_sort The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid
author_id_str_mv af160934b75bea5b8ba83d68b3d1a003
e98124f6e62b9592786899d7059e3a79
author_id_fullname_str_mv af160934b75bea5b8ba83d68b3d1a003_***_Christopher Coates
e98124f6e62b9592786899d7059e3a79_***_Andrew Rowley
author Christopher Coates
Andrew Rowley
author2 Christopher Coates
Jenson Lim
Katie Harman
Andrew Rowley
David J. Griffiths
Helena Emery
Will Layton
format Journal article
container_title Cell Biology and Toxicology
container_volume 35
container_start_page 219
publishDate 2019
institution Swansea University
issn 0742-2091
1573-6822
doi_str_mv 10.1007/s10565-018-09448-2
publisher Springer
college_str College of Science
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hierarchy_top_id collegeofscience
hierarchy_top_title College of Science
hierarchy_parent_id collegeofscience
hierarchy_parent_title College of Science
department_str Biosciences{{{_:::_}}}College of Science{{{_:::_}}}Biosciences
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description The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater waxmoth, Galleria mellonella, with physiologically relevant doses of okadaic acid by direct injection into the haemocoel (body cavity) and/or gavage (force-feeding). We monitored larval survival and employed a range of cellular and biochemical assays to assess the potential harmful effects of okadaic acid. Okadaic acid at concentrations >75 ng/larva (>242 µg/kg) led to significant reductions in larval survival (>65%) and circulating haemocyte (blood cell) numbers (>50%) within 24 h post-inoculation. In the haemolymph, okadaic acid reduced haemocyte viability and increased phenoloxidase activities. In the midgut, okadaic acid induced oxidative damage as determined by increases in superoxide dismutase activity and levels of malondialdehyde (i.e., lipid peroxidation). Our observations of insect larvae correspond broadly to data published using rodent models of shellfish poisoning toxidrome, including complementary LD50 values; 206–242 μg/kg in mice, ~239 μg/kg in G. mellonella. These data support the use of this insect as a surrogate model for the investigation of marine toxins, which offers distinct ethical and financial incentives.
published_date 2019-06-10T04:15:10Z
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