Journal article 1358 views 527 downloads
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
Cerys A. Jenkins,
Rhys A. Jenkins,
Meleri M. Pryse,
Kathryn A. Welsby,
Maki Jitsumura,
Cathy Thornton
,
Peter Dunstan
,
Dean A. Harris
The Analyst, Volume: 143, Issue: 24, Pages: 6014 - 6024
Swansea University Authors:
Cathy Thornton , Peter Dunstan
-
PDF | Accepted Manuscript
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DOI (Published version): 10.1039/c8an01323c
Abstract
Vibrational spectroscopic techniques such as Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR) have huge potential for the analysis of biological specimens. The techniques allow the user to gain label-free, non-destructive biochemical information about a given sample. Previous st...
Published in: | The Analyst |
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ISSN: | 0003-2654 1364-5528 |
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2018
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URI: | https://cronfa.swan.ac.uk/Record/cronfa45504 |
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2019-09-02T17:22:54.0944192 v2 45504 2018-11-08 A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false eada15d4d33fcb3dfddcff43f1323bd6 0000-0002-4337-4307 Peter Dunstan Peter Dunstan true false 2018-11-08 BMS Vibrational spectroscopic techniques such as Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR) have huge potential for the analysis of biological specimens. The techniques allow the user to gain label-free, non-destructive biochemical information about a given sample. Previous studies using vibrational spectroscopy with the specific application of diagnosing colorectal diseases such as cancer have mainly focused on in-vivo or in-vitro studies of tissue specimens using microscopy or probe based techniques. There have been few studies of vibrational spectroscopic techniques based on the analysis of blood serum for the advancement of colorectal cancer diagnostics. With growing interest in the field of liquid biopsies, this study presents the development of a high-throughput (HT) serum Raman spectroscopy platform and methodology and compares dry and liquid data acquisition of serum samples. This work considers factors contributing to translatability of the methodologies such as HT design, inter-user variability and sample handling effects on diagnostic capability. The HT Raman methods were tested on a pilot dataset of serum from 30 cancer patients and 30 matched control patients using statistical analysis via cross-validated PLS-DA with a maximum achieved a sensitivity of 83% and specificity of 83% for detecting colorectal cancer. Journal Article The Analyst 143 24 6014 6024 0003-2654 1364-5528 cancer; Raman spectroscopy 1 11 2018 2018-11-01 10.1039/c8an01323c COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-09-02T17:22:54.0944192 2018-11-08T11:09:55.1475028 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Cerys A. Jenkins 1 Rhys A. Jenkins 2 Meleri M. Pryse 3 Kathryn A. Welsby 4 Maki Jitsumura 5 Cathy Thornton 0000-0002-5153-573X 6 Peter Dunstan 0000-0002-4337-4307 7 Dean A. Harris 8 0045504-11122018084454.pdf 45504.pdf 2018-12-11T08:44:54.4400000 Output 25361189 application/pdf Accepted Manuscript true 2019-11-01T00:00:00.0000000 true eng |
title |
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics |
spellingShingle |
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics Cathy Thornton Peter Dunstan |
title_short |
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics |
title_full |
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics |
title_fullStr |
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics |
title_full_unstemmed |
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics |
title_sort |
A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics |
author_id_str_mv |
c71a7a4be7361094d046d312202bce0c eada15d4d33fcb3dfddcff43f1323bd6 |
author_id_fullname_str_mv |
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton eada15d4d33fcb3dfddcff43f1323bd6_***_Peter Dunstan |
author |
Cathy Thornton Peter Dunstan |
author2 |
Cerys A. Jenkins Rhys A. Jenkins Meleri M. Pryse Kathryn A. Welsby Maki Jitsumura Cathy Thornton Peter Dunstan Dean A. Harris |
format |
Journal article |
container_title |
The Analyst |
container_volume |
143 |
container_issue |
24 |
container_start_page |
6014 |
publishDate |
2018 |
institution |
Swansea University |
issn |
0003-2654 1364-5528 |
doi_str_mv |
10.1039/c8an01323c |
college_str |
Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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facultyofmedicinehealthandlifesciences |
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Faculty of Medicine, Health and Life Sciences |
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Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
Vibrational spectroscopic techniques such as Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR) have huge potential for the analysis of biological specimens. The techniques allow the user to gain label-free, non-destructive biochemical information about a given sample. Previous studies using vibrational spectroscopy with the specific application of diagnosing colorectal diseases such as cancer have mainly focused on in-vivo or in-vitro studies of tissue specimens using microscopy or probe based techniques. There have been few studies of vibrational spectroscopic techniques based on the analysis of blood serum for the advancement of colorectal cancer diagnostics. With growing interest in the field of liquid biopsies, this study presents the development of a high-throughput (HT) serum Raman spectroscopy platform and methodology and compares dry and liquid data acquisition of serum samples. This work considers factors contributing to translatability of the methodologies such as HT design, inter-user variability and sample handling effects on diagnostic capability. The HT Raman methods were tested on a pilot dataset of serum from 30 cancer patients and 30 matched control patients using statistical analysis via cross-validated PLS-DA with a maximum achieved a sensitivity of 83% and specificity of 83% for detecting colorectal cancer. |
published_date |
2018-11-01T03:57:18Z |
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1763752897582989312 |
score |
11.021648 |