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Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid

Paolo Bollella, Sanjiv Sharma Orcid Logo, Anthony Edward George Cass, Riccarda Antiochia

Electroanalysis, Volume: 31, Issue: 2, Pages: 374 - 382

Swansea University Author: Sanjiv Sharma Orcid Logo

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DOI (Published version): 10.1002/elan.201800630

Abstract

Here we report the first mediated pain free microneedle‐based biosensor array for the continuous and simultaneous monitoring of lactate and glucose in artificial interstitial fluid (ISF). The gold surface of the microneedles has been modified by electrodeposition of Au‐multiwalled carbon nanotubes (...

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Published in: Electroanalysis
ISSN: 10400397
Published: 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa48159
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first_indexed 2019-01-14T14:01:33Z
last_indexed 2019-09-25T14:11:58Z
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spelling 2019-09-25T10:11:09.4165351 v2 48159 2019-01-14 Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2019-01-14 MEDE Here we report the first mediated pain free microneedle‐based biosensor array for the continuous and simultaneous monitoring of lactate and glucose in artificial interstitial fluid (ISF). The gold surface of the microneedles has been modified by electrodeposition of Au‐multiwalled carbon nanotubes (MWCNTs) and successively by electropolymerization of the redox mediator, methylene blue (MB). Functionalization of the Au‐MWCNTs/polyMB platform with the lactate oxidase (LOX) enzyme (working electrode 1) and with the FAD‐Glucose dehydrogenase (FADGDH) enzyme (working electrode 2) enabled the continuous monitoring of lactate and glucose in the artificial ISF. The lactate biosensor exhibited a high sensitivity (797.4±38.1 μA cm−2 mM−1), a good linear range (10–100 μM) with a detection limit of 3 μM. The performance of the glucose biosensor were also good with a sensitivity of 405.2±24.1 μA cm−2 mM−1, a linear range between 0.05 and 5 mM and a detection limit of 7 μM. The biosensor array was tested to detect the amount of lactate generated after 100 minutes of cycling exercise (12 mM) and of glucose after a normal meal for a healthy patient (10 mM). The results reveal that the new microneedles‐based biosensor array seems to be a promising tool for the development of real‐time wearable devices with a variety of sport medicine and clinical care applications. Journal Article Electroanalysis 31 2 374 382 10400397 31 12 2019 2019-12-31 10.1002/elan.201800630 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2019-09-25T10:11:09.4165351 2019-01-14T11:17:17.3519688 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Paolo Bollella 1 Sanjiv Sharma 0000-0003-3828-737X 2 Anthony Edward George Cass 3 Riccarda Antiochia 4 0048159-14012019145943.pdf bollella2019.pdf 2019-01-14T14:59:43.1970000 Output 13190084 application/pdf Accepted Manuscript true 2020-01-10T00:00:00.0000000 true eng
title Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
spellingShingle Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
Sanjiv Sharma
title_short Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
title_full Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
title_fullStr Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
title_full_unstemmed Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
title_sort Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
author_id_str_mv b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma
author Sanjiv Sharma
author2 Paolo Bollella
Sanjiv Sharma
Anthony Edward George Cass
Riccarda Antiochia
format Journal article
container_title Electroanalysis
container_volume 31
container_issue 2
container_start_page 374
publishDate 2019
institution Swansea University
issn 10400397
doi_str_mv 10.1002/elan.201800630
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 1
active_str 0
description Here we report the first mediated pain free microneedle‐based biosensor array for the continuous and simultaneous monitoring of lactate and glucose in artificial interstitial fluid (ISF). The gold surface of the microneedles has been modified by electrodeposition of Au‐multiwalled carbon nanotubes (MWCNTs) and successively by electropolymerization of the redox mediator, methylene blue (MB). Functionalization of the Au‐MWCNTs/polyMB platform with the lactate oxidase (LOX) enzyme (working electrode 1) and with the FAD‐Glucose dehydrogenase (FADGDH) enzyme (working electrode 2) enabled the continuous monitoring of lactate and glucose in the artificial ISF. The lactate biosensor exhibited a high sensitivity (797.4±38.1 μA cm−2 mM−1), a good linear range (10–100 μM) with a detection limit of 3 μM. The performance of the glucose biosensor were also good with a sensitivity of 405.2±24.1 μA cm−2 mM−1, a linear range between 0.05 and 5 mM and a detection limit of 7 μM. The biosensor array was tested to detect the amount of lactate generated after 100 minutes of cycling exercise (12 mM) and of glucose after a normal meal for a healthy patient (10 mM). The results reveal that the new microneedles‐based biosensor array seems to be a promising tool for the development of real‐time wearable devices with a variety of sport medicine and clinical care applications.
published_date 2019-12-31T03:58:27Z
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