No Cover Image

Journal article 778 views 106 downloads

First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods

Dieter Lütjohann, Ingemar Björkhem, Silvia Friedrichs, Anja Kerksiek, Anita Lövgren-Sandblom, Wolf-Jochen Geilenkeuser, Robert Ahrends, Isabel Andrade, Diana Ansorena, Iciar Astiasarán, Lucía Baila-Rueda, Bianca Barriuso, Susen Becker, Lionel Bretillon, Richard W. Browne, Claudio Caccia, Uta Ceglarek, Ana Cenarro, Peter J. Crick, Günter Fauler, Guadalupe Garcia-Llatas, Robert Gray, William Griffiths Orcid Logo, Helena Gylling, Scott Harding, Christin Helmschrodt, Luigi Iuliano, Hans-Gerd Janssen, Peter Jones, Leena Kaipiainen, Frank Kannenberg, María Jesús Lagarda, Valerio Leoni, Ana Maria Lottenberg, Dylan S. MacKay, Silke Matysik, Jeff McDonald, Maria Menendez-Carreño, Semone B. Myrie, Valéria Sutti Nunes, Richard E. Ostlund, Eliana Polisecki, Fernando Ramos, Todd C. Rideout, Ernst J. Schaefer, Gerd Schmitz, Yuqin Wang, Chiara Zerbinati, Ulf Diczfalusy, Hans-Frieder Schött

The Journal of Steroid Biochemistry and Molecular Biology, Volume: 190, Pages: 115 - 125

Swansea University Author: William Griffiths Orcid Logo

  • 49899.pdf

    PDF | Version of Record

    Released under the terms of a Creative Commons Attribution License (CC-BY).

    Download (2.66MB)

Check full text

DOI (Published version): 10.1016/j.jsbmb.2019.03.025

Abstract

Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established...

Full description

Published in: The Journal of Steroid Biochemistry and Molecular Biology
ISSN: 09600760
Published: 2019
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa49899
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2019-04-08T10:16:42Z
last_indexed 2019-07-17T15:33:47Z
id cronfa49899
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2019-07-17T09:36:58.7001768</datestamp><bib-version>v2</bib-version><id>49899</id><entry>2019-04-05</entry><title>First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography&#x2013;Urgent need for harmonisation of analytical methods</title><swanseaauthors><author><sid>3316b1d1b524be1831790933eed1c26e</sid><ORCID>0000-0002-4129-6616</ORCID><firstname>William</firstname><surname>Griffiths</surname><name>William Griffiths</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2019-04-05</date><deptcode>BMS</deptcode><abstract>Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5&#x3B1;-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys,each laboratory used its own internal standard (5&#x3B1;-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs.</abstract><type>Journal Article</type><journal>The Journal of Steroid Biochemistry and Molecular Biology</journal><volume>190</volume><paginationStart>115</paginationStart><paginationEnd>125</paginationEnd><publisher/><issnPrint>09600760</issnPrint><keywords/><publishedDay>30</publishedDay><publishedMonth>6</publishedMonth><publishedYear>2019</publishedYear><publishedDate>2019-06-30</publishedDate><doi>10.1016/j.jsbmb.2019.03.025</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><degreesponsorsfunders>UKRI, BB/I001735/1, BB/L001942/1</degreesponsorsfunders><apcterm/><lastEdited>2019-07-17T09:36:58.7001768</lastEdited><Created>2019-04-05T08:57:11.9016530</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Dieter</firstname><surname>L&#xFC;tjohann</surname><order>1</order></author><author><firstname>Ingemar</firstname><surname>Bj&#xF6;rkhem</surname><order>2</order></author><author><firstname>Silvia</firstname><surname>Friedrichs</surname><order>3</order></author><author><firstname>Anja</firstname><surname>Kerksiek</surname><order>4</order></author><author><firstname>Anita</firstname><surname>L&#xF6;vgren-Sandblom</surname><order>5</order></author><author><firstname>Wolf-Jochen</firstname><surname>Geilenkeuser</surname><order>6</order></author><author><firstname>Robert</firstname><surname>Ahrends</surname><order>7</order></author><author><firstname>Isabel</firstname><surname>Andrade</surname><order>8</order></author><author><firstname>Diana</firstname><surname>Ansorena</surname><order>9</order></author><author><firstname>Iciar</firstname><surname>Astiasar&#xE1;n</surname><order>10</order></author><author><firstname>Luc&#xED;a</firstname><surname>Baila-Rueda</surname><order>11</order></author><author><firstname>Bianca</firstname><surname>Barriuso</surname><order>12</order></author><author><firstname>Susen</firstname><surname>Becker</surname><order>13</order></author><author><firstname>Lionel</firstname><surname>Bretillon</surname><order>14</order></author><author><firstname>Richard W.</firstname><surname>Browne</surname><order>15</order></author><author><firstname>Claudio</firstname><surname>Caccia</surname><order>16</order></author><author><firstname>Uta</firstname><surname>Ceglarek</surname><order>17</order></author><author><firstname>Ana</firstname><surname>Cenarro</surname><order>18</order></author><author><firstname>Peter J.</firstname><surname>Crick</surname><order>19</order></author><author><firstname>G&#xFC;nter</firstname><surname>Fauler</surname><order>20</order></author><author><firstname>Guadalupe</firstname><surname>Garcia-Llatas</surname><order>21</order></author><author><firstname>Robert</firstname><surname>Gray</surname><order>22</order></author><author><firstname>William</firstname><surname>Griffiths</surname><orcid>0000-0002-4129-6616</orcid><order>23</order></author><author><firstname>Helena</firstname><surname>Gylling</surname><order>24</order></author><author><firstname>Scott</firstname><surname>Harding</surname><order>25</order></author><author><firstname>Christin</firstname><surname>Helmschrodt</surname><order>26</order></author><author><firstname>Luigi</firstname><surname>Iuliano</surname><order>27</order></author><author><firstname>Hans-Gerd</firstname><surname>Janssen</surname><order>28</order></author><author><firstname>Peter</firstname><surname>Jones</surname><order>29</order></author><author><firstname>Leena</firstname><surname>Kaipiainen</surname><order>30</order></author><author><firstname>Frank</firstname><surname>Kannenberg</surname><order>31</order></author><author><firstname>Mar&#xED;a Jes&#xFA;s</firstname><surname>Lagarda</surname><order>32</order></author><author><firstname>Valerio</firstname><surname>Leoni</surname><order>33</order></author><author><firstname>Ana Maria</firstname><surname>Lottenberg</surname><order>34</order></author><author><firstname>Dylan S.</firstname><surname>MacKay</surname><order>35</order></author><author><firstname>Silke</firstname><surname>Matysik</surname><order>36</order></author><author><firstname>Jeff</firstname><surname>McDonald</surname><order>37</order></author><author><firstname>Maria</firstname><surname>Menendez-Carre&#xF1;o</surname><order>38</order></author><author><firstname>Semone B.</firstname><surname>Myrie</surname><order>39</order></author><author><firstname>Val&#xE9;ria</firstname><surname>Sutti Nunes</surname><order>40</order></author><author><firstname>Richard E.</firstname><surname>Ostlund</surname><order>41</order></author><author><firstname>Eliana</firstname><surname>Polisecki</surname><order>42</order></author><author><firstname>Fernando</firstname><surname>Ramos</surname><order>43</order></author><author><firstname>Todd C.</firstname><surname>Rideout</surname><order>44</order></author><author><firstname>Ernst J.</firstname><surname>Schaefer</surname><order>45</order></author><author><firstname>Gerd</firstname><surname>Schmitz</surname><order>46</order></author><author><firstname>Yuqin</firstname><surname>Wang</surname><order>47</order></author><author><firstname>Chiara</firstname><surname>Zerbinati</surname><order>48</order></author><author><firstname>Ulf</firstname><surname>Diczfalusy</surname><order>49</order></author><author><firstname>Hans-Frieder</firstname><surname>Sch&#xF6;tt</surname><order>50</order></author></authors><documents><document><filename>0049899-09052019112546.pdf</filename><originalFilename>49899.pdf</originalFilename><uploaded>2019-05-09T11:25:46.0200000</uploaded><type>Output</type><contentLength>2830019</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2019-05-08T00:00:00.0000000</embargoDate><documentNotes>Released under the terms of a Creative Commons Attribution License (CC-BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2019-07-17T09:36:58.7001768 v2 49899 2019-04-05 First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2019-04-05 BMS Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys,each laboratory used its own internal standard (5α-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs. Journal Article The Journal of Steroid Biochemistry and Molecular Biology 190 115 125 09600760 30 6 2019 2019-06-30 10.1016/j.jsbmb.2019.03.025 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University UKRI, BB/I001735/1, BB/L001942/1 2019-07-17T09:36:58.7001768 2019-04-05T08:57:11.9016530 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Dieter Lütjohann 1 Ingemar Björkhem 2 Silvia Friedrichs 3 Anja Kerksiek 4 Anita Lövgren-Sandblom 5 Wolf-Jochen Geilenkeuser 6 Robert Ahrends 7 Isabel Andrade 8 Diana Ansorena 9 Iciar Astiasarán 10 Lucía Baila-Rueda 11 Bianca Barriuso 12 Susen Becker 13 Lionel Bretillon 14 Richard W. Browne 15 Claudio Caccia 16 Uta Ceglarek 17 Ana Cenarro 18 Peter J. Crick 19 Günter Fauler 20 Guadalupe Garcia-Llatas 21 Robert Gray 22 William Griffiths 0000-0002-4129-6616 23 Helena Gylling 24 Scott Harding 25 Christin Helmschrodt 26 Luigi Iuliano 27 Hans-Gerd Janssen 28 Peter Jones 29 Leena Kaipiainen 30 Frank Kannenberg 31 María Jesús Lagarda 32 Valerio Leoni 33 Ana Maria Lottenberg 34 Dylan S. MacKay 35 Silke Matysik 36 Jeff McDonald 37 Maria Menendez-Carreño 38 Semone B. Myrie 39 Valéria Sutti Nunes 40 Richard E. Ostlund 41 Eliana Polisecki 42 Fernando Ramos 43 Todd C. Rideout 44 Ernst J. Schaefer 45 Gerd Schmitz 46 Yuqin Wang 47 Chiara Zerbinati 48 Ulf Diczfalusy 49 Hans-Frieder Schött 50 0049899-09052019112546.pdf 49899.pdf 2019-05-09T11:25:46.0200000 Output 2830019 application/pdf Version of Record true 2019-05-08T00:00:00.0000000 Released under the terms of a Creative Commons Attribution License (CC-BY). true eng
title First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
spellingShingle First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
William Griffiths
title_short First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
title_full First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
title_fullStr First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
title_full_unstemmed First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
title_sort First international descriptive and interventional survey for cholesterol and non-cholesterol sterol determination by gas- and liquid-chromatography–Urgent need for harmonisation of analytical methods
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author William Griffiths
author2 Dieter Lütjohann
Ingemar Björkhem
Silvia Friedrichs
Anja Kerksiek
Anita Lövgren-Sandblom
Wolf-Jochen Geilenkeuser
Robert Ahrends
Isabel Andrade
Diana Ansorena
Iciar Astiasarán
Lucía Baila-Rueda
Bianca Barriuso
Susen Becker
Lionel Bretillon
Richard W. Browne
Claudio Caccia
Uta Ceglarek
Ana Cenarro
Peter J. Crick
Günter Fauler
Guadalupe Garcia-Llatas
Robert Gray
William Griffiths
Helena Gylling
Scott Harding
Christin Helmschrodt
Luigi Iuliano
Hans-Gerd Janssen
Peter Jones
Leena Kaipiainen
Frank Kannenberg
María Jesús Lagarda
Valerio Leoni
Ana Maria Lottenberg
Dylan S. MacKay
Silke Matysik
Jeff McDonald
Maria Menendez-Carreño
Semone B. Myrie
Valéria Sutti Nunes
Richard E. Ostlund
Eliana Polisecki
Fernando Ramos
Todd C. Rideout
Ernst J. Schaefer
Gerd Schmitz
Yuqin Wang
Chiara Zerbinati
Ulf Diczfalusy
Hans-Frieder Schött
format Journal article
container_title The Journal of Steroid Biochemistry and Molecular Biology
container_volume 190
container_start_page 115
publishDate 2019
institution Swansea University
issn 09600760
doi_str_mv 10.1016/j.jsbmb.2019.03.025
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Serum concentrations of lathosterol, the plant sterols campesterol and sitosterol and the cholesterol metabolite 5α-cholestanol are widely used as surrogate markers of cholesterol synthesis and absorption, respectively. Increasing numbers of laboratories utilize a broad spectrum of well-established and recently developed methods for the determination of cholesterol and non-cholesterol sterols (NCS). In order to evaluate the quality of these measurements and to identify possible sources of analytical errors our group initiated the first international survey for cholesterol and NCS. The cholesterol and NCS survey was structured as a two-part survey which took place in the years 2013 and 2014. The first survey part was designed as descriptive, providing information about the variation of reported results from different laboratories. A set of two lyophilized pooled sera (A and B) was sent to twenty laboratories specialized in chromatographic lipid analysis. The different sterols were quantified either by gas chromatography-flame ionization detection, gas chromatography- or liquid chromatography-mass selective detection. The participants were requested to determine cholesterol and NCS concentrations in the provided samples as part of their normal laboratory routine. The second part was designed as interventional survey. Twenty-two laboratories agreed to participate and received again two different lyophilized pooled sera (C and D). In contrast to the first international survey, each participant received standard stock solutions with defined concentrations of cholesterol and NCS. The participants were requested to use diluted calibration solutions from the provided standard stock solutions for quantification of cholesterol and NCS. In both surveys,each laboratory used its own internal standard (5α-cholestane, epicoprostanol or deuterium labelled sterols). Main outcome of the survey was, that unacceptably high interlaboratory variations for cholesterol and NCS concentrations are reported, even when the individual laboratories used the same calibration material. We discuss different sources of errors and recommend all laboratories analysing cholesterol and NCS to participate in regular quality control programs.
published_date 2019-06-30T04:01:09Z
_version_ 1763753139807191040
score 11.016235

Similar Items