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Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection

Lydia Powell Orcid Logo, Aled E. L. Roberts, Lydia C. Powell, Manon F. Pritchard, David W. Thomas, Rowena Jenkins Orcid Logo

Frontiers in Microbiology, Volume: 10

Swansea University Authors: Lydia Powell Orcid Logo, Rowena Jenkins Orcid Logo

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DOI (Published version): 10.3389/fmicb.2019.00869

Abstract

Chronic lung infections involving Pseudomonas aeruginosa in people with cystic fibrosis are common. These infections are often formed of bacteria with high levels of antibiotic resistance which form biofilms within the lung. The limited treatment options for these infections means that new antimicro...

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Published in: Frontiers in Microbiology
ISSN: 1664-302X
Published: 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa50103
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first_indexed 2019-05-09T20:00:51Z
last_indexed 2019-07-16T21:32:23Z
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spelling 2019-07-16T16:12:12.3615514 v2 50103 2019-04-29 Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection 0e7e702952672bcbfdfd4974199202fb 0000-0002-8641-0160 Lydia Powell Lydia Powell true false caf58cd775a27bf8b9ee340079a29cc0 0000-0001-6664-6099 Rowena Jenkins Rowena Jenkins true false 2019-04-29 BMS Chronic lung infections involving Pseudomonas aeruginosa in people with cystic fibrosis are common. These infections are often formed of bacteria with high levels of antibiotic resistance which form biofilms within the lung. The limited treatment options for these infections means that new antimicrobial strategies are required. Manuka honey has been used successfully to treat topical infections in wounds for many years. In this study the potential for manuka honey to inhibit pulmonary isolates of Pseudomonas aeruginosa alone or in combination with antibiotics was assessed in an ex vivo porcine lung model. Twenty eight strains of Pseudomonas were tested using conventional antibiotic susceptibility testing with ciprofloxacin, ceftazidime and tobramycin as well as in two ex vivo porcine lung models. All the isolates were susceptible to manuka honey using conventional testing where as many of the isolates displayed resistance to the antibiotics tested. In conventional testing where the bacteria are not in biofilms form combining manuka honey and antibiotics had little effect on inhibition. In the ex vivo models where bacteria were in biofilm form (more similar to a chronic lung infection) many of the isolates were not inhibited by antibiotics even at greatly elevated levels but were inhibited by manuka honey. When antibiotics and manuka honey were combined in the ex vivo models the ability to inhibit bacteria was improved in most cases. Journal Article Frontiers in Microbiology 10 1664-302X Pseudomonas aeruginosa, biofilms, antimicrobial susceptibility testing, manuka honey, ex vivo model, cystic fibrosis 24 4 2019 2019-04-24 10.3389/fmicb.2019.00869 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-07-16T16:12:12.3615514 2019-04-29T09:09:20.2702419 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Lydia Powell 0000-0002-8641-0160 1 Aled E. L. Roberts 2 Lydia C. Powell 3 Manon F. Pritchard 4 David W. Thomas 5 Rowena Jenkins 0000-0001-6664-6099 6 0050103-15052019122259.pdf 50103.pdf 2019-05-15T12:22:59.0430000 Output 4782802 application/pdf Version of Record true 2019-05-14T00:00:00.0000000 Released under the terms of a Creative Commons Attribution License (CC-BY). true eng
title Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection
spellingShingle Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection
Lydia Powell
Rowena Jenkins
title_short Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection
title_full Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection
title_fullStr Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection
title_full_unstemmed Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection
title_sort Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection
author_id_str_mv 0e7e702952672bcbfdfd4974199202fb
caf58cd775a27bf8b9ee340079a29cc0
author_id_fullname_str_mv 0e7e702952672bcbfdfd4974199202fb_***_Lydia Powell
caf58cd775a27bf8b9ee340079a29cc0_***_Rowena Jenkins
author Lydia Powell
Rowena Jenkins
author2 Lydia Powell
Aled E. L. Roberts
Lydia C. Powell
Manon F. Pritchard
David W. Thomas
Rowena Jenkins
format Journal article
container_title Frontiers in Microbiology
container_volume 10
publishDate 2019
institution Swansea University
issn 1664-302X
doi_str_mv 10.3389/fmicb.2019.00869
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description Chronic lung infections involving Pseudomonas aeruginosa in people with cystic fibrosis are common. These infections are often formed of bacteria with high levels of antibiotic resistance which form biofilms within the lung. The limited treatment options for these infections means that new antimicrobial strategies are required. Manuka honey has been used successfully to treat topical infections in wounds for many years. In this study the potential for manuka honey to inhibit pulmonary isolates of Pseudomonas aeruginosa alone or in combination with antibiotics was assessed in an ex vivo porcine lung model. Twenty eight strains of Pseudomonas were tested using conventional antibiotic susceptibility testing with ciprofloxacin, ceftazidime and tobramycin as well as in two ex vivo porcine lung models. All the isolates were susceptible to manuka honey using conventional testing where as many of the isolates displayed resistance to the antibiotics tested. In conventional testing where the bacteria are not in biofilms form combining manuka honey and antibiotics had little effect on inhibition. In the ex vivo models where bacteria were in biofilm form (more similar to a chronic lung infection) many of the isolates were not inhibited by antibiotics even at greatly elevated levels but were inhibited by manuka honey. When antibiotics and manuka honey were combined in the ex vivo models the ability to inhibit bacteria was improved in most cases.
published_date 2019-04-24T04:01:25Z
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