Journal article 162 views
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
Stefano Perni,
Lirong Yang,
Emily Preedy 
,
Polina Prokopovich
,
Polina Prokopovich
Journal of Colloid and Interface Science, Volume: 531, Pages: 410 - 420
Swansea University Author:
Emily Preedy
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1016/j.jcis.2018.07.028
Abstract
We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investiga...
| Published in: | Journal of Colloid and Interface Science |
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| ISSN: | 0021-9797 |
| Published: |
2018
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa51202 |
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| Abstract: |
We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells.Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at concentrations up to 1 mg/ml and by Cobalt nanoparticles at concentrations < 0.5 mg/ml; however elasticity and spring constant were significantly modified by the exposure to nanoparticles of these metals in agreement with the alteration of cell conformation (shape), as result of the exposure to simulated wear debris, demonstrated by fluorescence images after actin staining. |
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| Keywords: |
Osteoblast, Cobalt, Titanium, Nanoparticles, Wear debris |
| College: |
Faculty of Science and Engineering |
| Start Page: |
410 |
| End Page: |
420 |