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Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton

Stefano Perni, Lirong Yang, Emily Preedy Orcid Logo, Polina Prokopovich

Journal of Colloid and Interface Science, Volume: 531, Pages: 410 - 420

Swansea University Author: Emily Preedy Orcid Logo

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DOI (Published version): 10.1016/j.jcis.2018.07.028

Abstract

We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investiga...

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Published in: Journal of Colloid and Interface Science
ISSN: 0021-9797
Published: 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa51202
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first_indexed 2019-07-25T17:48:21Z
last_indexed 2019-08-09T16:31:36Z
id cronfa51202
recordtype SURis
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spelling 2019-08-07T11:39:16.4623141 v2 51202 2019-07-25 Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton a4ae2d29d6b017b303c85efa3a9503d0 0000-0003-0377-6747 Emily Preedy Emily Preedy true false 2019-07-25 CHEG We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells.Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at concentrations up to 1 mg/ml and by Cobalt nanoparticles at concentrations < 0.5 mg/ml; however elasticity and spring constant were significantly modified by the exposure to nanoparticles of these metals in agreement with the alteration of cell conformation (shape), as result of the exposure to simulated wear debris, demonstrated by fluorescence images after actin staining. Journal Article Journal of Colloid and Interface Science 531 410 420 0021-9797 Osteoblast, Cobalt, Titanium, Nanoparticles, Wear debris 1 12 2018 2018-12-01 10.1016/j.jcis.2018.07.028 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University 2019-08-07T11:39:16.4623141 2019-07-25T14:10:49.7258022 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Stefano Perni 1 Lirong Yang 2 Emily Preedy 0000-0003-0377-6747 3 Polina Prokopovich 4
title Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
spellingShingle Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
Emily Preedy
title_short Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
title_full Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
title_fullStr Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
title_full_unstemmed Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
title_sort Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
author_id_str_mv a4ae2d29d6b017b303c85efa3a9503d0
author_id_fullname_str_mv a4ae2d29d6b017b303c85efa3a9503d0_***_Emily Preedy
author Emily Preedy
author2 Stefano Perni
Lirong Yang
Emily Preedy
Polina Prokopovich
format Journal article
container_title Journal of Colloid and Interface Science
container_volume 531
container_start_page 410
publishDate 2018
institution Swansea University
issn 0021-9797
doi_str_mv 10.1016/j.jcis.2018.07.028
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 0
active_str 0
description We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells.Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at concentrations up to 1 mg/ml and by Cobalt nanoparticles at concentrations < 0.5 mg/ml; however elasticity and spring constant were significantly modified by the exposure to nanoparticles of these metals in agreement with the alteration of cell conformation (shape), as result of the exposure to simulated wear debris, demonstrated by fluorescence images after actin staining.
published_date 2018-12-01T04:03:00Z
_version_ 1763753255823736832
score 11.016392

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