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Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro

Feihu Zhao Orcid Logo, Bert van Rietbergen, Keita Ito, Sandra Hofmann

Journal of Biomechanics, Volume: 79, Pages: 232 - 237

Swansea University Author: Feihu Zhao Orcid Logo

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DOI (Published version): 10.1016/j.jbiomech.2018.08.004

Abstract

In bone tissue engineering experiments, fluid-induced shear stress can stimulate cells to produce mineralised extracellular matrix (ECM). The application of shear stress on seeded cells can for example be achieved through bioreactors that perfuse medium through porous scaffolds. The generated mechan...

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Published in: Journal of Biomechanics
ISSN: 0021-9290
Published: 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa51681
first_indexed 2019-09-04T20:46:37Z
last_indexed 2020-06-26T19:03:09Z
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spelling 2020-06-26T16:07:12.3750477 v2 51681 2019-09-04 Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2019-09-04 EAAS In bone tissue engineering experiments, fluid-induced shear stress can stimulate cells to produce mineralised extracellular matrix (ECM). The application of shear stress on seeded cells can for example be achieved through bioreactors that perfuse medium through porous scaffolds. The generated mechanical environment (i.e. wall shear stress: WSS) within the scaffolds is complex due to the complexity of scaffold geometry. This complexity has prevented setting an optimal loading (i.e. flow rate) of the bioreactor to achieve an optimal distribution of WSS for stimulating cells to produce mineralised ECM. In this study, a combination of computational fluid dynamics approach with mechano-regulation theory was employed to optimise the external flow rate for a perfusion bioreactor. Such flow rate would maximise the scaffold surface fraction, whose WSS was in the range required for mineralisation. As expected, the optimal flow rate was dependent on the scaffold geometry, in particular on the scaffold porosity. However, it was in a reasonable range of 0.5–5 mL/min (or in terms of fluid velocity: 0.166–1.66 mm/s) for the bioreactor used in our study. It is expected that this approach can lead to the reduction of pilot studies required to find optimal loading conditions as well as to a better insight into the parameters that determine the mineralisation within scaffolds. Journal Article Journal of Biomechanics 79 232 237 0021-9290 Computational fluid dynamics, wall shear stress, mechanical stimulation, bone tissue mineralisation 5 10 2018 2018-10-05 10.1016/j.jbiomech.2018.08.004 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University 2020-06-26T16:07:12.3750477 2019-09-04T15:40:47.2729545 Feihu Zhao 0000-0003-0515-6808 1 Bert van Rietbergen 2 Keita Ito 3 Sandra Hofmann 4 0051681-07092019011038.pdf JBiomech_Zhao.pdf 2019-09-07T01:10:38.5130000 Output 1399546 application/pdf Corrected Version of Record true This article is released under Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) true eng https://creativecommons.org/licenses/by-nc-nd/4.0/
title Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
spellingShingle Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
Feihu Zhao
title_short Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
title_full Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
title_fullStr Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
title_full_unstemmed Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
title_sort Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
author_id_str_mv 1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv 1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author Feihu Zhao
author2 Feihu Zhao
Bert van Rietbergen
Keita Ito
Sandra Hofmann
format Journal article
container_title Journal of Biomechanics
container_volume 79
container_start_page 232
publishDate 2018
institution Swansea University
issn 0021-9290
doi_str_mv 10.1016/j.jbiomech.2018.08.004
document_store_str 1
active_str 0
description In bone tissue engineering experiments, fluid-induced shear stress can stimulate cells to produce mineralised extracellular matrix (ECM). The application of shear stress on seeded cells can for example be achieved through bioreactors that perfuse medium through porous scaffolds. The generated mechanical environment (i.e. wall shear stress: WSS) within the scaffolds is complex due to the complexity of scaffold geometry. This complexity has prevented setting an optimal loading (i.e. flow rate) of the bioreactor to achieve an optimal distribution of WSS for stimulating cells to produce mineralised ECM. In this study, a combination of computational fluid dynamics approach with mechano-regulation theory was employed to optimise the external flow rate for a perfusion bioreactor. Such flow rate would maximise the scaffold surface fraction, whose WSS was in the range required for mineralisation. As expected, the optimal flow rate was dependent on the scaffold geometry, in particular on the scaffold porosity. However, it was in a reasonable range of 0.5–5 mL/min (or in terms of fluid velocity: 0.166–1.66 mm/s) for the bioreactor used in our study. It is expected that this approach can lead to the reduction of pilot studies required to find optimal loading conditions as well as to a better insight into the parameters that determine the mineralisation within scaffolds.
published_date 2018-10-05T13:48:07Z
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score 11.048453

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