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Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
Journal of Biomechanics, Volume: 79, Pages: 232 - 237
Swansea University Author:
Feihu Zhao
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DOI (Published version): 10.1016/j.jbiomech.2018.08.004
Abstract
In bone tissue engineering experiments, fluid-induced shear stress can stimulate cells to produce mineralised extracellular matrix (ECM). The application of shear stress on seeded cells can for example be achieved through bioreactors that perfuse medium through porous scaffolds. The generated mechan...
Published in: | Journal of Biomechanics |
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ISSN: | 0021-9290 |
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2018
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URI: | https://cronfa.swan.ac.uk/Record/cronfa51681 |
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2020-06-26T16:07:12.3750477 v2 51681 2019-09-04 Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2019-09-04 MEDE In bone tissue engineering experiments, fluid-induced shear stress can stimulate cells to produce mineralised extracellular matrix (ECM). The application of shear stress on seeded cells can for example be achieved through bioreactors that perfuse medium through porous scaffolds. The generated mechanical environment (i.e. wall shear stress: WSS) within the scaffolds is complex due to the complexity of scaffold geometry. This complexity has prevented setting an optimal loading (i.e. flow rate) of the bioreactor to achieve an optimal distribution of WSS for stimulating cells to produce mineralised ECM. In this study, a combination of computational fluid dynamics approach with mechano-regulation theory was employed to optimise the external flow rate for a perfusion bioreactor. Such flow rate would maximise the scaffold surface fraction, whose WSS was in the range required for mineralisation. As expected, the optimal flow rate was dependent on the scaffold geometry, in particular on the scaffold porosity. However, it was in a reasonable range of 0.5–5 mL/min (or in terms of fluid velocity: 0.166–1.66 mm/s) for the bioreactor used in our study. It is expected that this approach can lead to the reduction of pilot studies required to find optimal loading conditions as well as to a better insight into the parameters that determine the mineralisation within scaffolds. Journal Article Journal of Biomechanics 79 232 237 0021-9290 Computational fluid dynamics, wall shear stress, mechanical stimulation, bone tissue mineralisation 5 10 2018 2018-10-05 10.1016/j.jbiomech.2018.08.004 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2020-06-26T16:07:12.3750477 2019-09-04T15:40:47.2729545 Feihu Zhao 0000-0003-0515-6808 1 Bert van Rietbergen 2 Keita Ito 3 Sandra Hofmann 4 0051681-07092019011038.pdf JBiomech_Zhao.pdf 2019-09-07T01:10:38.5130000 Output 1399546 application/pdf Corrected Version of Record true This article is released under Creative Commons License Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) true eng https://creativecommons.org/licenses/by-nc-nd/4.0/ |
title |
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro |
spellingShingle |
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro Feihu Zhao |
title_short |
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro |
title_full |
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro |
title_fullStr |
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro |
title_full_unstemmed |
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro |
title_sort |
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro |
author_id_str_mv |
1c6e79b6edd08c88a8d17a241cd78630 |
author_id_fullname_str_mv |
1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao |
author |
Feihu Zhao |
author2 |
Feihu Zhao Bert van Rietbergen Keita Ito Sandra Hofmann |
format |
Journal article |
container_title |
Journal of Biomechanics |
container_volume |
79 |
container_start_page |
232 |
publishDate |
2018 |
institution |
Swansea University |
issn |
0021-9290 |
doi_str_mv |
10.1016/j.jbiomech.2018.08.004 |
document_store_str |
1 |
active_str |
0 |
description |
In bone tissue engineering experiments, fluid-induced shear stress can stimulate cells to produce mineralised extracellular matrix (ECM). The application of shear stress on seeded cells can for example be achieved through bioreactors that perfuse medium through porous scaffolds. The generated mechanical environment (i.e. wall shear stress: WSS) within the scaffolds is complex due to the complexity of scaffold geometry. This complexity has prevented setting an optimal loading (i.e. flow rate) of the bioreactor to achieve an optimal distribution of WSS for stimulating cells to produce mineralised ECM. In this study, a combination of computational fluid dynamics approach with mechano-regulation theory was employed to optimise the external flow rate for a perfusion bioreactor. Such flow rate would maximise the scaffold surface fraction, whose WSS was in the range required for mineralisation. As expected, the optimal flow rate was dependent on the scaffold geometry, in particular on the scaffold porosity. However, it was in a reasonable range of 0.5–5 mL/min (or in terms of fluid velocity: 0.166–1.66 mm/s) for the bioreactor used in our study. It is expected that this approach can lead to the reduction of pilot studies required to find optimal loading conditions as well as to a better insight into the parameters that determine the mineralisation within scaffolds. |
published_date |
2018-10-05T04:03:40Z |
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1763753297700716544 |
score |
11.021648 |