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Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation

Johanna Melke, Feihu Zhao Orcid Logo, Bert van Rietbergen, Keita Ito, Sandra Hofmann

European Cells and Materials, Volume: 36, Pages: 57 - 68

Swansea University Author: Feihu Zhao Orcid Logo

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DOI (Published version): 10.22203/eCM.v036a05

Abstract

Spinner flask bioreactors have widely been used in bone tissue engineering. However, the underlying reason for their success in facilitating bone growth remain unclear. It was hypothesised that engineered bone tissue formation can be attributed to mechanical stimuli, which can be predicted in the ti...

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Published in: European Cells and Materials
ISSN: 1473-2262
Published: 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa51682
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first_indexed 2019-09-04T20:46:37Z
last_indexed 2020-06-26T19:03:09Z
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spelling 2020-06-26T16:02:27.8786291 v2 51682 2019-09-04 Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2019-09-04 MEDE Spinner flask bioreactors have widely been used in bone tissue engineering. However, the underlying reason for their success in facilitating bone growth remain unclear. It was hypothesised that engineered bone tissue formation can be attributed to mechanical stimuli, which can be predicted in the tissue engineered construct. To test the hypothesis and draw conclusions as to how mechanical stimulation affects cell behaviour, a multidisciplinary approach using cell culture experiments and computational fluid dynamics (CFD) to simulate the complex flow within the spinner flask and scaffold was employed. Micro-computed tomography and histology showed that statically cultured human bone marrow derived stromal cells on silk fibroin scaffolds did not form extracellular matrix (ECM) or deposit minerals. However, constructs cultured at 60 rpm resulted in ECM formation and mineralisation, mainly at the bottom of the scaffold (bottom: 78±7%, middle: 17±5%, top: 5±2% of total mineralised volume). Culturing at 300 rpm led to a more homogeneously distributed ECM (bottom: 40±14%, middle: 33±1%, top: 27±14% of total mineralised volume). These observations were in agreement (Pearson correlation coefficient: 97%) with the computational simulations that predicted maximal scaffold mineralisation, based on wall shear stress stimulation, in the bottom at 60 rpm and in the main body at 300 rpm. Such combinations of CFD modelling and experimentation could advance our knowledge of the mechanical stimuli that cells experience in vitro and link them to biological responses. Journal Article European Cells and Materials 36 57 68 1473-2262 Bone tissue engineering, spinner flask bioreactor, computational fluid dynamics, scaffold 31 7 2018 2018-07-31 10.22203/eCM.v036a05 http://www.ecmjournal.org/papers/vol036/vol036a05.php COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2020-06-26T16:02:27.8786291 2019-09-04T15:40:49.0749202 Johanna Melke 1 Feihu Zhao 0000-0003-0515-6808 2 Bert van Rietbergen 3 Keita Ito 4 Sandra Hofmann 5 0051682-07092019011514.pdf eCM_Melkeetal2018.pdf 2019-09-07T01:15:14.7230000 Output 2537638 application/pdf Corrected Version of Record true 2019-09-07T00:00:00.0000000 This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by-sa/4.0/). false eng http://creativecommons.org/licenses/by-sa/4.0
title Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
spellingShingle Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
Feihu Zhao
title_short Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
title_full Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
title_fullStr Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
title_full_unstemmed Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
title_sort Localisation of mineralised tissue in a complex spinner flask environment correlates with predicted wall shear stress level localisation
author_id_str_mv 1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv 1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author Feihu Zhao
author2 Johanna Melke
Feihu Zhao
Bert van Rietbergen
Keita Ito
Sandra Hofmann
format Journal article
container_title European Cells and Materials
container_volume 36
container_start_page 57
publishDate 2018
institution Swansea University
issn 1473-2262
doi_str_mv 10.22203/eCM.v036a05
url http://www.ecmjournal.org/papers/vol036/vol036a05.php
document_store_str 1
active_str 0
description Spinner flask bioreactors have widely been used in bone tissue engineering. However, the underlying reason for their success in facilitating bone growth remain unclear. It was hypothesised that engineered bone tissue formation can be attributed to mechanical stimuli, which can be predicted in the tissue engineered construct. To test the hypothesis and draw conclusions as to how mechanical stimulation affects cell behaviour, a multidisciplinary approach using cell culture experiments and computational fluid dynamics (CFD) to simulate the complex flow within the spinner flask and scaffold was employed. Micro-computed tomography and histology showed that statically cultured human bone marrow derived stromal cells on silk fibroin scaffolds did not form extracellular matrix (ECM) or deposit minerals. However, constructs cultured at 60 rpm resulted in ECM formation and mineralisation, mainly at the bottom of the scaffold (bottom: 78±7%, middle: 17±5%, top: 5±2% of total mineralised volume). Culturing at 300 rpm led to a more homogeneously distributed ECM (bottom: 40±14%, middle: 33±1%, top: 27±14% of total mineralised volume). These observations were in agreement (Pearson correlation coefficient: 97%) with the computational simulations that predicted maximal scaffold mineralisation, based on wall shear stress stimulation, in the bottom at 60 rpm and in the main body at 300 rpm. Such combinations of CFD modelling and experimentation could advance our knowledge of the mechanical stimuli that cells experience in vitro and link them to biological responses.
published_date 2018-07-31T04:03:40Z
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score 11.016235

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