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Resolving a clinical tuberculosis outbreak using palaeogenomic genome reconstruction methodologies / Rhys Jones, Marcela Sandoval Velasco, Llinos G. Harris, Sue Morgan, Mark Temple, Michael C Ruddy, Rhian Williams, Michael D Perry, Matt Hitchings, Thomas Wilkinson, Thomas Humphrey, M. Thomas P. Gilbert, Angharad Davies

Tuberculosis, Volume: 119, Start page: 101865

Swansea University Authors: Thomas Wilkinson, Angharad Davies

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Abstract

This study describes the analysis of DNA from heat-killed (boilate) isolates of Mycobacterium tuberculosis from two UK outbreaks where DNA was of sub-optimal quality for the standard methodologies routinely used in microbial genomics. An Illumina library construction method developed for sequencing...

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Published in: Tuberculosis
ISSN: 14729792
Published: 2019
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa51995
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Abstract: This study describes the analysis of DNA from heat-killed (boilate) isolates of Mycobacterium tuberculosis from two UK outbreaks where DNA was of sub-optimal quality for the standard methodologies routinely used in microbial genomics. An Illumina library construction method developed for sequencing ancient DNA was successfully used to obtain whole genome sequences, allowing analysis of the outbreak by gene-by-gene MLST, SNP mapping and phylogenetic analysis. All cases were spoligotyped to the same Haarlem H1 sub-lineage. This is the first described application of ancient DNA library construction protocols to allow whole genome sequencing of a clinical tuberculosis outbreak. Using this method it is possible to obtain epidemiologically meaningful data even when DNA is of insufficient quality for standard methods.
Keywords: Mycobacterium tuberculosis, whole genome sequencing, outbreak investigation, ancient DNA library construction, palaeogenomics
College: Swansea University Medical School
Start Page: 101865