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Optimization of dietary RNA interference delivery to western flower thrips Frankliniella occidentalis and onion thrips Thrips tabaci

Awawing Anjwengwo Andongma, Carolyn Greig, Paul Dyson Orcid Logo, Natasha Flynn, Miranda Walker

Archives of Insect Biochemistry and Physiology, Volume: 103, Issue: 3

Swansea University Authors: Awawing Anjwengwo Andongma, Carolyn Greig, Paul Dyson Orcid Logo, Miranda Walker

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DOI (Published version): 10.1002/arch.21645

Abstract

The article describes techniques for oral delivery of RNAi in the agricultural pests Western Flower thrips and onion thrips. We focus on diet optimization and a comparison of oral delivery methods of dsRNA, including symbiont-mediated RNAi which is the technique pioneered by our group. This is essen...

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Published in: Archives of Insect Biochemistry and Physiology
ISSN: 0739-4462 1520-6327
Published: Wiley 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa52659
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Abstract: The article describes techniques for oral delivery of RNAi in the agricultural pests Western Flower thrips and onion thrips. We focus on diet optimization and a comparison of oral delivery methods of dsRNA, including symbiont-mediated RNAi which is the technique pioneered by our group. This is essentially a methods paper that provides key information for researchers planning reverse genetics experiments and biocontrol strategies in thrips and related pest species.
Keywords: feeding dsRNA, diet modification, Onion thrips, Western flower thrips, RNAi
College: Swansea University Medical School
Funders: UKRI, BB/R006148/1
Issue: 3