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A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions / D.A. García-López, E.J. Olguín, R.E. González-Portela, G. Sánchez-Galván, R. De Philippis, Robert Lovitt, Carole Llewellyn, Claudio Fuentes Grunewald, R. Parra Saldívar

Bioresource Technology, Volume: 298, Start page: 122548

Swansea University Authors: Robert Lovitt, Carole Llewellyn, Claudio Fuentes Grunewald

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Abstract

A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions, was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was har...

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Published in: Bioresource Technology
ISSN: 0960-8524
Published: Elsevier BV 2020
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa53021
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Abstract: A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions, was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was harvested and concentrated using membrane technology at the end of each cycle for further phycocyanin induction using blue LED light (controlled conditions, 24h). The highest biomass productivity was observed during spring and summer cycles; during mid-fall and mid-end fall, a decrease was observed. Under favorable growth conditions, phycocyanin induction was successful. However, during cycles with unfavorable growth condition, phycocyanin induction was not observed. Reagent grade phycocyanin was recovered and purified using microfiltration and ultrafiltration technologies. It was concluded that this novel two phase process has a potential to be implemented at larger scale within a biorefinery approach including the production of sub products from residual biomass and exopolysaccharides.
Keywords: Cyanobacteria; Pigments induction; Membrane harvesting; Membrane ultrafiltration; Urea
College: College of Science
Start Page: 122548