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A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions

D.A. García-López, E.J. Olguín, R.E. González-Portela, G. Sánchez-Galván, R. De Philippis, Robert Lovitt, Carole Llewellyn, Claudio Fuentes Grunewald Orcid Logo, R. Parra Saldívar

Bioresource Technology, Volume: 298, Start page: 122548

Swansea University Authors: Robert Lovitt , Carole Llewellyn , Claudio Fuentes Grunewald Orcid Logo

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Abstract

A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions, was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was har...

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Published in: Bioresource Technology
ISSN: 0960-8524
Published: Elsevier BV 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa53021
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spelling 2020-11-18T13:41:08.6471709 v2 53021 2019-12-11 A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions 130c3c35f45826bb0f4836305e8e51c7 Robert Lovitt Robert Lovitt true false bcd94bda79ebf4c2c82d82dfb027a140 Carole Llewellyn Carole Llewellyn true false 8d7cf97e82178c021883618d24acb4b4 0000-0002-3122-9452 Claudio Fuentes Grunewald Claudio Fuentes Grunewald true false 2019-12-11 FGSEN A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions, was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was harvested and concentrated using membrane technology at the end of each cycle for further phycocyanin induction using blue LED light (controlled conditions, 24h). The highest biomass productivity was observed during spring and summer cycles; during mid-fall and mid-end fall, a decrease was observed. Under favorable growth conditions, phycocyanin induction was successful. However, during cycles with unfavorable growth condition, phycocyanin induction was not observed. Reagent grade phycocyanin was recovered and purified using microfiltration and ultrafiltration technologies. It was concluded that this novel two phase process has a potential to be implemented at larger scale within a biorefinery approach including the production of sub products from residual biomass and exopolysaccharides. Journal Article Bioresource Technology 298 122548 Elsevier BV 0960-8524 Cyanobacteria; Pigments induction; Membrane harvesting; Membrane ultrafiltration; Urea 1 2 2020 2020-02-01 10.1016/j.biortech.2019.122548 COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University 2020-11-18T13:41:08.6471709 2019-12-11T14:59:46.9291225 College of Science Biosciences D.A. García-López 1 E.J. Olguín 2 R.E. González-Portela 3 G. Sánchez-Galván 4 R. De Philippis 5 Robert Lovitt 6 Carole Llewellyn 7 Claudio Fuentes Grunewald 0000-0002-3122-9452 8 R. Parra Saldívar 9 53021__16231__b9edfee4d3494dcf9507f7bd88354375.pdf 53021.pdf 2020-01-08T10:07:25.4866543 Output 920341 application/pdf Accepted Manuscript true 2020-12-04T00:00:00.0000000 Released under the terms of a Creative Commons Attribution Non-Commercial No Derivatives License (CC-BY-NC-ND). true eng https://creativecommons.org/licenses/by-nc-nd/4.0/
title A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
spellingShingle A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
Robert, Lovitt
Carole, Llewellyn
Claudio, Fuentes Grunewald
title_short A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
title_full A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
title_fullStr A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
title_full_unstemmed A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
title_sort A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions
author_id_str_mv 130c3c35f45826bb0f4836305e8e51c7
bcd94bda79ebf4c2c82d82dfb027a140
8d7cf97e82178c021883618d24acb4b4
author_id_fullname_str_mv 130c3c35f45826bb0f4836305e8e51c7_***_Robert, Lovitt_***_
bcd94bda79ebf4c2c82d82dfb027a140_***_Carole, Llewellyn_***_
8d7cf97e82178c021883618d24acb4b4_***_Claudio, Fuentes Grunewald_***_0000-0002-3122-9452
author Robert, Lovitt
Carole, Llewellyn
Claudio, Fuentes Grunewald
author2 D.A. García-López
E.J. Olguín
R.E. González-Portela
G. Sánchez-Galván
R. De Philippis
Robert Lovitt
Carole Llewellyn
Claudio Fuentes Grunewald
R. Parra Saldívar
format Journal article
container_title Bioresource Technology
container_volume 298
container_start_page 122548
publishDate 2020
institution Swansea University
issn 0960-8524
doi_str_mv 10.1016/j.biortech.2019.122548
publisher Elsevier BV
college_str College of Science
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hierarchy_top_id collegeofscience
hierarchy_top_title College of Science
hierarchy_parent_id collegeofscience
hierarchy_parent_title College of Science
department_str Biosciences{{{_:::_}}}College of Science{{{_:::_}}}Biosciences
document_store_str 1
active_str 0
description A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions, was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was harvested and concentrated using membrane technology at the end of each cycle for further phycocyanin induction using blue LED light (controlled conditions, 24h). The highest biomass productivity was observed during spring and summer cycles; during mid-fall and mid-end fall, a decrease was observed. Under favorable growth conditions, phycocyanin induction was successful. However, during cycles with unfavorable growth condition, phycocyanin induction was not observed. Reagent grade phycocyanin was recovered and purified using microfiltration and ultrafiltration technologies. It was concluded that this novel two phase process has a potential to be implemented at larger scale within a biorefinery approach including the production of sub products from residual biomass and exopolysaccharides.
published_date 2020-02-01T04:17:31Z
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