Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells

Swithenbank, Lucy; COX, PHILLIPA; Harris, Llinos; Dudley, Ed; Coates, Kathryn; Lewis, Paul; Cappiello, Floriana; Morgan, Claire

doi:10.1155/2020/3526286

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Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells

Lucy Swithenbank, PHILLIPA COX, Llinos Harris

, Ed Dudley, Kathryn Coates, Paul Lewis, Floriana Cappiello, Claire Morgan

Scientifica, Volume: 2020, Pages: 1 - 10

Swansea University Authors: Lucy Swithenbank, PHILLIPA COX, Llinos Harris , Ed Dudley, Kathryn Coates, Paul Lewis, Claire Morgan

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DOI (Published version): 10.1155/2020/3526286

Abstract

Background. Recently, antimicrobial peptides (AMPs) have been investigated for their use in cancer therapy. They have been reported to selectively target and kill cancer cells whilst leaving normal healthy cells unaffected. Certain Anura AMPs have expressed selective cytotoxicity against tumour cell...

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Published in:	Scientifica
ISSN:	2090-908X
Published:	Hindawi Limited 2020
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa54358

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Recently, antimicrobial peptides (AMPs) have been investigated for their use in cancer therapy. They have been reported to selectively target and kill cancer cells whilst leaving normal healthy cells unaffected. Certain Anura AMPs have expressed selective cytotoxicity against tumour cells. Aim. To test the potential of Anura AMPs bombinin H2, bombinin H4, temporin A, and temporin L for use as therapeutic agents for non-small cell lung carcinoma (NSCLC). Methods. Cytotoxic effects on NSCLC cell lines A549 and Calu-3 and normal epithelial cell line Beas-2B were tested using the CellTox Green Cytotoxicity Assay. Their haemolytic effects on human erythrocytes were also tested for their clinical relevance. Cell membrane profiling, using MALDI-TOF, was performed to ascertain if membrane characteristics of the NSCLC and Beas-2B cell lines may contribute to the AMPs mode of action. Results. Bombinin H4 (100–1.5 μM, p < 0.05) and temporin A (100–50 μM, p < 0.05) showed selective cytotoxicity towards the NSCLC cell lines. Furthermore, they exhibited low levels of haemolytic activity (bombinin H4, 0.061%; temporin A, 0.874%) comparable to untreated cells. Cell membrane profiling showed the phospholipid composition of normal epithelial cell line Beas-2B to be divergent from the cancerous cell lines. However, there was an overlap in the phospholipid profiles of the NSCLC cell lines supporting the hypothesis that the AMPs may have a selective affinity via the membrane composition of cancerous cell lines. Conclusion. These results suggest that bombinin H4 and temporin A show potential for application in lung cancer therapies. 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spelling	2025-04-07T12:39:20.9248323 v2 54358 2020-06-01 Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells 46855a445121e6fe61835a01fae0c8ae Lucy Swithenbank Lucy Swithenbank true false c772a61a7ab7afbf8d1be3a27c6ff47c PHILLIPA COX PHILLIPA COX true false dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false c7d05f992a817cd3b9a5f946bd909b71 Ed Dudley Ed Dudley true false c98a9f7646a8c9360de034071d3992de Kathryn Coates Kathryn Coates true false 46dfc22d7468f247c390ba0c6cd8fba6 Paul Lewis Paul Lewis true false 52c886f26c1b4d9d817000ac6e58a486 0000-0002-3969-0710 Claire Morgan Claire Morgan true false 2020-06-01 MEDS Background. Recently, antimicrobial peptides (AMPs) have been investigated for their use in cancer therapy. They have been reported to selectively target and kill cancer cells whilst leaving normal healthy cells unaffected. Certain Anura AMPs have expressed selective cytotoxicity against tumour cells. Aim. To test the potential of Anura AMPs bombinin H2, bombinin H4, temporin A, and temporin L for use as therapeutic agents for non-small cell lung carcinoma (NSCLC). Methods. Cytotoxic effects on NSCLC cell lines A549 and Calu-3 and normal epithelial cell line Beas-2B were tested using the CellTox Green Cytotoxicity Assay. Their haemolytic effects on human erythrocytes were also tested for their clinical relevance. Cell membrane profiling, using MALDI-TOF, was performed to ascertain if membrane characteristics of the NSCLC and Beas-2B cell lines may contribute to the AMPs mode of action. Results. Bombinin H4 (100–1.5 μM, p < 0.05) and temporin A (100–50 μM, p < 0.05) showed selective cytotoxicity towards the NSCLC cell lines. Furthermore, they exhibited low levels of haemolytic activity (bombinin H4, 0.061%; temporin A, 0.874%) comparable to untreated cells. Cell membrane profiling showed the phospholipid composition of normal epithelial cell line Beas-2B to be divergent from the cancerous cell lines. However, there was an overlap in the phospholipid profiles of the NSCLC cell lines supporting the hypothesis that the AMPs may have a selective affinity via the membrane composition of cancerous cell lines. Conclusion. These results suggest that bombinin H4 and temporin A show potential for application in lung cancer therapies. Further in vitro and in vivo studies are required to develop a greater understanding of their use as anticancer agents. Journal Article Scientifica 2020 1 10 Hindawi Limited 2090-908X 29 6 2020 2020-06-29 10.1155/2020/3526286 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2025-04-07T12:39:20.9248323 2020-06-01T10:24:10.6059437 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Lucy Swithenbank 1 PHILLIPA COX 2 Llinos Harris 0000-0002-0295-3038 3 Ed Dudley 4 Kathryn Coates 5 Paul Lewis 6 Floriana Cappiello 7 Claire Morgan 0000-0002-3969-0710 8 54358__17774__e63405a6bc1c451aa28929d440d06a17.pdf 54358.pdf 2020-07-23T14:48:25.3982321 Output 2259159 application/pdf Version of Record true This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. true
title	Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells
spellingShingle	Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells Lucy Swithenbank PHILLIPA COX Llinos Harris Ed Dudley Kathryn Coates Paul Lewis Claire Morgan
title_short	Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells
title_full	Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells
title_fullStr	Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells
title_full_unstemmed	Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells
title_sort	Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells
author_id_str_mv	46855a445121e6fe61835a01fae0c8ae c772a61a7ab7afbf8d1be3a27c6ff47c dc70f9d4badbbdb5d467fd321986d173 c7d05f992a817cd3b9a5f946bd909b71 c98a9f7646a8c9360de034071d3992de 46dfc22d7468f247c390ba0c6cd8fba6 52c886f26c1b4d9d817000ac6e58a486
author_id_fullname_str_mv	46855a445121e6fe61835a01fae0c8ae_*_Lucy Swithenbank c772a61a7ab7afbf8d1be3a27c6ff47c__PHILLIPA COX dc70f9d4badbbdb5d467fd321986d173__Llinos Harris c7d05f992a817cd3b9a5f946bd909b71__Ed Dudley c98a9f7646a8c9360de034071d3992de__Kathryn Coates 46dfc22d7468f247c390ba0c6cd8fba6__Paul Lewis 52c886f26c1b4d9d817000ac6e58a486_**_Claire Morgan
author	Lucy Swithenbank PHILLIPA COX Llinos Harris Ed Dudley Kathryn Coates Paul Lewis Claire Morgan
author2	Lucy Swithenbank PHILLIPA COX Llinos Harris Ed Dudley Kathryn Coates Paul Lewis Floriana Cappiello Claire Morgan
format	Journal article
container_title	Scientifica
container_volume	2020
container_start_page	1
publishDate	2020
institution	Swansea University
issn	2090-908X
doi_str_mv	10.1155/2020/3526286
publisher	Hindawi Limited
college_str	Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
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hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
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description	Background. Recently, antimicrobial peptides (AMPs) have been investigated for their use in cancer therapy. They have been reported to selectively target and kill cancer cells whilst leaving normal healthy cells unaffected. Certain Anura AMPs have expressed selective cytotoxicity against tumour cells. Aim. To test the potential of Anura AMPs bombinin H2, bombinin H4, temporin A, and temporin L for use as therapeutic agents for non-small cell lung carcinoma (NSCLC). Methods. Cytotoxic effects on NSCLC cell lines A549 and Calu-3 and normal epithelial cell line Beas-2B were tested using the CellTox Green Cytotoxicity Assay. Their haemolytic effects on human erythrocytes were also tested for their clinical relevance. Cell membrane profiling, using MALDI-TOF, was performed to ascertain if membrane characteristics of the NSCLC and Beas-2B cell lines may contribute to the AMPs mode of action. Results. Bombinin H4 (100–1.5 μM, p < 0.05) and temporin A (100–50 μM, p < 0.05) showed selective cytotoxicity towards the NSCLC cell lines. Furthermore, they exhibited low levels of haemolytic activity (bombinin H4, 0.061%; temporin A, 0.874%) comparable to untreated cells. Cell membrane profiling showed the phospholipid composition of normal epithelial cell line Beas-2B to be divergent from the cancerous cell lines. However, there was an overlap in the phospholipid profiles of the NSCLC cell lines supporting the hypothesis that the AMPs may have a selective affinity via the membrane composition of cancerous cell lines. Conclusion. These results suggest that bombinin H4 and temporin A show potential for application in lung cancer therapies. Further in vitro and in vivo studies are required to develop a greater understanding of their use as anticancer agents.
published_date	2020-06-29T06:06:27Z
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Temporin A and Bombinin H2 Antimicrobial Peptides Exhibit Selective Cytotoxicity to Lung Cancer Cells

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