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A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids

Jessica Minett, Carlos Garcia De Leaniz Orcid Logo, Paul Brickle Orcid Logo, Sofia Consuegra del Olmo Orcid Logo

Environmental DNA, Volume: 3, Issue: 3, Pages: 561 - 572

Swansea University Authors: Jessica Minett, Carlos Garcia De Leaniz Orcid Logo, Sofia Consuegra del Olmo Orcid Logo

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DOI (Published version): 10.1002/edn3.151

Abstract

Brown trout are highly invasive in the Southern Hemisphere where they support important sport fisheries and aquaculture activities, which may impact endangered native galaxiid fishes and cause conflicts. To protect native galaxiids it is essential to monitor changes in species distributions, but thi...

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Published in: Environmental DNA
ISSN: 2637-4943 2637-4943
Published: Wiley 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa56109
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spelling 2022-10-10T10:30:09.9035084 v2 56109 2021-01-24 A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids 8e592c5576ea17cb28d83f8dc45bd932 Jessica Minett Jessica Minett true false 1c70acd0fd64edb0856b7cf34393ab02 0000-0003-1650-2729 Carlos Garcia De Leaniz Carlos Garcia De Leaniz true false 241f2810ab8f56be53ca8af23e384c6e 0000-0003-4403-2509 Sofia Consuegra del Olmo Sofia Consuegra del Olmo true false 2021-01-24 Brown trout are highly invasive in the Southern Hemisphere where they support important sport fisheries and aquaculture activities, which may impact endangered native galaxiid fishes and cause conflicts. To protect native galaxiids it is essential to monitor changes in species distributions, but this can be difficult when species are rare or difficult to sample. We developed and validated, both in the laboratory and in the field, a new assay using a high-resolution melt curve (HRM)-eDNA approach to simultaneously detect the presence of two threatened native galaxiids (Aplochiton zebra and Aplochiton taeniatus) and the invasive brown trout (Salmo trutta). Using this method, we found brown trout in 30% of the sampled waterbodies and Aplochiton sp. in 15% of them. Galaxiids were solely identified as being present in rivers that lacked brown trout, with both native species coexisting in two of the three rivers where they were detected, despite their different niche preferences. These assays can be used to monitor threatened zebra trout as well as invasive brown trout populations, allowing conservation managers to target areas for intervention. Journal Article Environmental DNA 3 3 561 572 Wiley 2637-4943 2637-4943 Aplochiton taeniatus; Aplochiton zebra; endemic fishes; high‐resolution melt curve; invasive species; qPCR‐HRM 1 5 2021 2021-05-01 10.1002/edn3.151 COLLEGE NANME COLLEGE CODE Swansea University Fortuna Ltd; Swansea University 2022-10-10T10:30:09.9035084 2021-01-24T11:42:05.5625219 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Jessica Minett 1 Carlos Garcia De Leaniz 0000-0003-1650-2729 2 Paul Brickle 0000-0002-9870-3518 3 Sofia Consuegra del Olmo 0000-0003-4403-2509 4 56109__19297__6862dc9ecf004b27b531675b837a8e6e.pdf 56109.pdf 2021-02-15T12:03:11.1914464 Output 1390996 application/pdf Version of Record true © 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License true eng http://creativecommons.org/licenses/by-nc-nd/4.0/
title A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
spellingShingle A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
Jessica Minett
Carlos Garcia De Leaniz
Sofia Consuegra del Olmo
title_short A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
title_full A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
title_fullStr A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
title_full_unstemmed A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
title_sort A new high‐resolution melt curve eDNA assay to monitor the simultaneous presence of invasive brown trout ( <i>Salmo trutta</i> ) and endangered galaxiids
author_id_str_mv 8e592c5576ea17cb28d83f8dc45bd932
1c70acd0fd64edb0856b7cf34393ab02
241f2810ab8f56be53ca8af23e384c6e
author_id_fullname_str_mv 8e592c5576ea17cb28d83f8dc45bd932_***_Jessica Minett
1c70acd0fd64edb0856b7cf34393ab02_***_Carlos Garcia De Leaniz
241f2810ab8f56be53ca8af23e384c6e_***_Sofia Consuegra del Olmo
author Jessica Minett
Carlos Garcia De Leaniz
Sofia Consuegra del Olmo
author2 Jessica Minett
Carlos Garcia De Leaniz
Paul Brickle
Sofia Consuegra del Olmo
format Journal article
container_title Environmental DNA
container_volume 3
container_issue 3
container_start_page 561
publishDate 2021
institution Swansea University
issn 2637-4943
2637-4943
doi_str_mv 10.1002/edn3.151
publisher Wiley
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description Brown trout are highly invasive in the Southern Hemisphere where they support important sport fisheries and aquaculture activities, which may impact endangered native galaxiid fishes and cause conflicts. To protect native galaxiids it is essential to monitor changes in species distributions, but this can be difficult when species are rare or difficult to sample. We developed and validated, both in the laboratory and in the field, a new assay using a high-resolution melt curve (HRM)-eDNA approach to simultaneously detect the presence of two threatened native galaxiids (Aplochiton zebra and Aplochiton taeniatus) and the invasive brown trout (Salmo trutta). Using this method, we found brown trout in 30% of the sampled waterbodies and Aplochiton sp. in 15% of them. Galaxiids were solely identified as being present in rivers that lacked brown trout, with both native species coexisting in two of the three rivers where they were detected, despite their different niche preferences. These assays can be used to monitor threatened zebra trout as well as invasive brown trout populations, allowing conservation managers to target areas for intervention.
published_date 2021-05-01T04:10:49Z
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