An Organoid for Woven Bone

Akiva, Anat; Melke, Johanna; Ansari, Sana; Liv, Nalan; Meijden, Robin; Erp, Merijn; Zhao, Feihu; Stout, Merula; Nijhuis, Wouter H.; Heus, Cilia; Ortera, Claudia Muñiz; Fermie, Job; Klumperman, Judith; Ito, Keita; Sommerdijk, Nico; Hofmann, Sandra

doi:10.1002/adfm.202010524

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An Organoid for Woven Bone

Anat Akiva, Johanna Melke, Sana Ansari, Nalan Liv, Robin Meijden, Merijn Erp, Feihu Zhao

, Merula Stout, Wouter H. Nijhuis, Cilia Heus, Claudia Muñiz Ortera, Job Fermie, Judith Klumperman, Keita Ito, Nico Sommerdijk, Sandra Hofmann

Advanced Functional Materials, Volume: 31, Issue: 17, Start page: 2010524

Swansea University Author: Feihu Zhao

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© 2021 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. The copyright line for this article was changed on 27 April 2021 after original online publication.
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DOI (Published version): 10.1002/adfm.202010524

Abstract

Bone formation (osteogenesis) is a complex process in which cellular differentiation and the generation of a mineralized organic matrix are synchronized to produce a hybrid hierarchical architecture. To study the mechanisms of osteogenesis in health and disease, there is a great need for functional...

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Published in:	Advanced Functional Materials
ISSN:	1616-301X 1616-3028
Published:	Wiley 2021
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URI:	https://cronfa.swan.ac.uk/Record/cronfa56465
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first_indexed	2021-03-18T10:01:53Z
last_indexed	2023-01-11T14:35:42Z
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Stem cell‐based organoids are promising as functional, self‐organizing 3D in vitro models for studying the physiology and pathology of various tissues. However, for human bone, no such functional model system is yet available. This study reports the in vitro differentiation of human bone marrow stromal cells into a functional 3D self‐organizing co‐culture of osteoblasts and osteocytes, creating an organoid for early stage bone (woven bone) formation. It demonstrates the formation of an organoid where osteocytes are embedded within the collagen matrix that is produced by the osteoblasts and mineralized under biological control. Alike in in vivo osteocytes, the embedded osteocytes show network formation and communication via expression of sclerostin. 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spelling	2022-10-31T18:50:29.3424349 v2 56465 2021-03-18 An Organoid for Woven Bone 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2021-03-18 MEDE Bone formation (osteogenesis) is a complex process in which cellular differentiation and the generation of a mineralized organic matrix are synchronized to produce a hybrid hierarchical architecture. To study the mechanisms of osteogenesis in health and disease, there is a great need for functional model systems that capture in parallel, both cellular and matrix formation processes. Stem cell‐based organoids are promising as functional, self‐organizing 3D in vitro models for studying the physiology and pathology of various tissues. However, for human bone, no such functional model system is yet available. This study reports the in vitro differentiation of human bone marrow stromal cells into a functional 3D self‐organizing co‐culture of osteoblasts and osteocytes, creating an organoid for early stage bone (woven bone) formation. It demonstrates the formation of an organoid where osteocytes are embedded within the collagen matrix that is produced by the osteoblasts and mineralized under biological control. Alike in in vivo osteocytes, the embedded osteocytes show network formation and communication via expression of sclerostin. The current system forms the most complete 3D living in vitro model system to investigate osteogenesis, both in physiological and pathological situations, as well as under the influence of external triggers (mechanical stimulation, drug administration). Journal Article Advanced Functional Materials 31 17 2010524 Wiley 1616-301X 1616-3028 1 4 2021 2021-04-01 10.1002/adfm.202010524 http://dx.doi.org/10.1002/adfm.202010524 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University Another institution paid the OA fee Marie Curie Individual Fellowship. Grant Number: H2020-MSCA-IF-2017-794296-SUPERMIN Netherlands Organization for Scientific Research 2022-10-31T18:50:29.3424349 2021-03-18T10:00:49.4919332 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Anat Akiva 1 Johanna Melke 2 Sana Ansari 3 Nalan Liv 4 Robin Meijden 5 Merijn Erp 6 Feihu Zhao 0000-0003-0515-6808 7 Merula Stout 8 Wouter H. Nijhuis 9 Cilia Heus 10 Claudia Muñiz Ortera 11 Job Fermie 12 Judith Klumperman 13 Keita Ito 14 Nico Sommerdijk 15 Sandra Hofmann 16 56465__20210__e68a616512a24ec695ac3e2a0aa8d060.pdf 56465.VOR with CC-BY.pdf 2021-06-21T16:47:05.8705306 Output 3519528 application/pdf Version of Record true © 2021 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. The copyright line for this article was changed on 27 April 2021 after original online publication. true eng http://creativecommons.org/licenses/by/4.0/
title	An Organoid for Woven Bone
spellingShingle	An Organoid for Woven Bone Feihu Zhao
title_short	An Organoid for Woven Bone
title_full	An Organoid for Woven Bone
title_fullStr	An Organoid for Woven Bone
title_full_unstemmed	An Organoid for Woven Bone
title_sort	An Organoid for Woven Bone
author_id_str_mv	1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv	1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author	Feihu Zhao
author2	Anat Akiva Johanna Melke Sana Ansari Nalan Liv Robin Meijden Merijn Erp Feihu Zhao Merula Stout Wouter H. Nijhuis Cilia Heus Claudia Muñiz Ortera Job Fermie Judith Klumperman Keita Ito Nico Sommerdijk Sandra Hofmann
format	Journal article
container_title	Advanced Functional Materials
container_volume	31
container_issue	17
container_start_page	2010524
publishDate	2021
institution	Swansea University
issn	1616-301X 1616-3028
doi_str_mv	10.1002/adfm.202010524
publisher	Wiley
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department_str	School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
url	http://dx.doi.org/10.1002/adfm.202010524
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description	Bone formation (osteogenesis) is a complex process in which cellular differentiation and the generation of a mineralized organic matrix are synchronized to produce a hybrid hierarchical architecture. To study the mechanisms of osteogenesis in health and disease, there is a great need for functional model systems that capture in parallel, both cellular and matrix formation processes. Stem cell‐based organoids are promising as functional, self‐organizing 3D in vitro models for studying the physiology and pathology of various tissues. However, for human bone, no such functional model system is yet available. This study reports the in vitro differentiation of human bone marrow stromal cells into a functional 3D self‐organizing co‐culture of osteoblasts and osteocytes, creating an organoid for early stage bone (woven bone) formation. It demonstrates the formation of an organoid where osteocytes are embedded within the collagen matrix that is produced by the osteoblasts and mineralized under biological control. Alike in in vivo osteocytes, the embedded osteocytes show network formation and communication via expression of sclerostin. The current system forms the most complete 3D living in vitro model system to investigate osteogenesis, both in physiological and pathological situations, as well as under the influence of external triggers (mechanical stimulation, drug administration).
published_date	2021-04-01T04:11:27Z
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An Organoid for Woven Bone

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