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Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology / BETHAN KULTSCHAR

Swansea University Author: BETHAN KULTSCHAR

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DOI (Published version): 10.23889/SUthesis.57241

Abstract

Cyanobacteria produce a variety of metabolites with diverse functions and bioactive properties that have potential uses in industrial biotechnology. Metabolomic profiles closely relate to the physiology of an organism and can be used to investigate any alteration in metabolism and production of indu...

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Published: Swansea 2020
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
Supervisor: Llewellyn, Carole. ; Dudley, Ed ; Wilson, Steve
URI: https://cronfa.swan.ac.uk/Record/cronfa57241
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first_indexed 2021-06-29T13:00:02Z
last_indexed 2021-06-30T03:23:49Z
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spelling 2021-06-29T14:47:22.5126009 v2 57241 2021-06-29 Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology 6b195bb2fdf5fdbca7d4a3326dbfdfc7 BETHAN KULTSCHAR BETHAN KULTSCHAR true false 2021-06-29 Cyanobacteria produce a variety of metabolites with diverse functions and bioactive properties that have potential uses in industrial biotechnology. Metabolomic profiles closely relate to the physiology of an organism and can be used to investigate any alteration in metabolism and production of industrially relevant metabolites. Little work has been conducted on metabolomic time-course profiles within cyanobacteria with the majority of research on targeted metabolite level changes during abiotic stress such as ultraviolet (UV) radiation. In this thesis the metabolite profile analysis of the less well investigated cyanobacterium; Chlorogloeopsis fritschii (C. fritschii) PCC 6912 was undertaken. The main aim was to evaluate changes in low molecular weight metabolite levels during standard growth and UV exposure using gas chromatography-mass spectrometry (GC-MS). The focus was on identifying biologically relevant metabolites with roles in cyanobacterial metabolism associated with growth phases and adaptation to UV stress. Extracts were assessed using in vitro assays for extraction of potential anti-inflammatory and antibacterial activity from the cyanobacteria with complementary metabolomic analysis utilised for identification of potential active metabolites. Results demonstrated a significant reduction in intracellular metabolites involved in carbon and nitrogen metabolism during UV-B exposure with a higher proportion of metabolites increasing in levels during UV-A exposure. Pre-treatment of C. fritschii to low dose UV prior to high dose UV had a lesser effect on metabolism indicating stress tolerance and adaptation to UV stress. Metabolite levels were seen to reflect the changing growth phases over 120 days during standard conditions with overall similarity in metabolite levels between axenic and xenic cultures over 28 days. Finally, extracts showed promising anti-inflammatory and antibacterial activity with identification of potential active metabolites with relevance to personal care products. This is the first GC-MS based metabolite profiling of C. fritschii during standard growth and UV exposure. This builds on the experimental data and knowledge-base already available for cyanobacteria, including C. fritschii, for their potential use in industrial biotechnology. E-Thesis Swansea Cyanobacteria, metabolite profiling, industrial biotechnology, ultraviolet radiation, mass spectrometry 23 4 2020 2020-04-23 10.23889/SUthesis.57241 COLLEGE NANME COLLEGE CODE Swansea University Llewellyn, Carole. ; Dudley, Ed ; Wilson, Steve Doctoral Ph.D Unilever 2021-06-29T14:47:22.5126009 2021-06-29T13:57:27.4682846 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences BETHAN KULTSCHAR 1 57241__20296__17f687814c994df3b2b2a1e91b612d8b.pdf Kultschar_Bethan_PhD_Thesis_Final_Redacted_Signature.pdf 2021-06-29T14:16:00.7656779 Output 8287393 application/pdf E-Thesis – open access true Copyright: The author, Bethan Kultschar, 2020. true eng
title Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology
spellingShingle Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology
BETHAN KULTSCHAR
title_short Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology
title_full Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology
title_fullStr Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology
title_full_unstemmed Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology
title_sort Metabolite Profiling of a Robust Cyanobacterium for Industrial biotechnology
author_id_str_mv 6b195bb2fdf5fdbca7d4a3326dbfdfc7
author_id_fullname_str_mv 6b195bb2fdf5fdbca7d4a3326dbfdfc7_***_BETHAN KULTSCHAR
author BETHAN KULTSCHAR
author2 BETHAN KULTSCHAR
format E-Thesis
publishDate 2020
institution Swansea University
doi_str_mv 10.23889/SUthesis.57241
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Cyanobacteria produce a variety of metabolites with diverse functions and bioactive properties that have potential uses in industrial biotechnology. Metabolomic profiles closely relate to the physiology of an organism and can be used to investigate any alteration in metabolism and production of industrially relevant metabolites. Little work has been conducted on metabolomic time-course profiles within cyanobacteria with the majority of research on targeted metabolite level changes during abiotic stress such as ultraviolet (UV) radiation. In this thesis the metabolite profile analysis of the less well investigated cyanobacterium; Chlorogloeopsis fritschii (C. fritschii) PCC 6912 was undertaken. The main aim was to evaluate changes in low molecular weight metabolite levels during standard growth and UV exposure using gas chromatography-mass spectrometry (GC-MS). The focus was on identifying biologically relevant metabolites with roles in cyanobacterial metabolism associated with growth phases and adaptation to UV stress. Extracts were assessed using in vitro assays for extraction of potential anti-inflammatory and antibacterial activity from the cyanobacteria with complementary metabolomic analysis utilised for identification of potential active metabolites. Results demonstrated a significant reduction in intracellular metabolites involved in carbon and nitrogen metabolism during UV-B exposure with a higher proportion of metabolites increasing in levels during UV-A exposure. Pre-treatment of C. fritschii to low dose UV prior to high dose UV had a lesser effect on metabolism indicating stress tolerance and adaptation to UV stress. Metabolite levels were seen to reflect the changing growth phases over 120 days during standard conditions with overall similarity in metabolite levels between axenic and xenic cultures over 28 days. Finally, extracts showed promising anti-inflammatory and antibacterial activity with identification of potential active metabolites with relevance to personal care products. This is the first GC-MS based metabolite profiling of C. fritschii during standard growth and UV exposure. This builds on the experimental data and knowledge-base already available for cyanobacteria, including C. fritschii, for their potential use in industrial biotechnology.
published_date 2020-04-23T04:12:49Z
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score 11.035655

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