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Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans

P. K. Pradhan, Dev Kumar Verma, Luca Peruzza, Shubham Gupta, Syed Assim Haq, Sergei Shubin Orcid Logo, Kenton L. Morgan, Franziska Trusch, Vindhya Mohindra, Chris Hauton, Pieter van West, Neeraj Sood

Scientific Reports, Volume: 10, Issue: 1

Swansea University Author: Sergei Shubin Orcid Logo

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DOI (Published version): 10.1038/s41598-020-76278-w

Abstract

Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: Springer Science and Business Media LLC 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa57712
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spelling 2021-09-20T14:57:55.9635804 v2 57712 2021-08-30 Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans 2944e02dc0e6e0ba376aea2c8575b682 0000-0001-5554-816X Sergei Shubin Sergei Shubin true false 2021-08-30 SGE Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major impacts globally. In particular, Indian major carps (IMCs) are highly susceptible to this disease. To increase our knowledge particularly with regards to host immune response against A. invadans infection in a susceptible host, the gene expression profile in head kidney of A. invadans-infected and control rohu, Labeo rohita was investigated using RNA sequencing. Time course analysis of RNA-Seq data revealed 5608 differentially expressed genes, involved among others in Antigen processing and presentation, Leukocyte transendothelial migration, IL-17 signaling, Chemokine signaling, C-type lectin receptor signaling and Toll-like receptor signaling pathways. In the affected pathways, a number of immune genes were found to be downregulated, suggesting an immune evasion strategy of A. invadans in establishing the infection. The information generated in this study offers first systematic mechanistic understanding of the host-pathogen interaction that might underpin the development of new management strategies for this economically devastating fish-pathogenic oomycete A. invadans. Journal Article Scientific Reports 10 1 Springer Science and Business Media LLC 2045-2322 11 11 2020 2020-11-11 10.1038/s41598-020-76278-w COLLEGE NANME Geography COLLEGE CODE SGE Swansea University 2021-09-20T14:57:55.9635804 2021-08-30T15:04:26.0149735 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences P. K. Pradhan 1 Dev Kumar Verma 2 Luca Peruzza 3 Shubham Gupta 4 Syed Assim Haq 5 Sergei Shubin 0000-0001-5554-816X 6 Kenton L. Morgan 7 Franziska Trusch 8 Vindhya Mohindra 9 Chris Hauton 10 Pieter van West 11 Neeraj Sood 12 57712__20720__9e4d34fee1b548a189f846b0c57ff5f1.pdf 57712.VOR.s41598-020-76278-w.pdf 2021-08-30T15:08:17.9649121 Output 4776058 application/pdf Version of Record true © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/
title Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
spellingShingle Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
Sergei Shubin
title_short Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_full Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_fullStr Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_full_unstemmed Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
title_sort Molecular insights into the mechanisms of susceptibility of Labeo rohita against oomycete Aphanomyces invadans
author_id_str_mv 2944e02dc0e6e0ba376aea2c8575b682
author_id_fullname_str_mv 2944e02dc0e6e0ba376aea2c8575b682_***_Sergei Shubin
author Sergei Shubin
author2 P. K. Pradhan
Dev Kumar Verma
Luca Peruzza
Shubham Gupta
Syed Assim Haq
Sergei Shubin
Kenton L. Morgan
Franziska Trusch
Vindhya Mohindra
Chris Hauton
Pieter van West
Neeraj Sood
format Journal article
container_title Scientific Reports
container_volume 10
container_issue 1
publishDate 2020
institution Swansea University
issn 2045-2322
doi_str_mv 10.1038/s41598-020-76278-w
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome, is one of the most destructive pathogens of freshwater fishes. To date, the disease has been reported from over 160 fish species in 20 countries and notably, this is the first non-salmonid disease that has resulted in major impacts globally. In particular, Indian major carps (IMCs) are highly susceptible to this disease. To increase our knowledge particularly with regards to host immune response against A. invadans infection in a susceptible host, the gene expression profile in head kidney of A. invadans-infected and control rohu, Labeo rohita was investigated using RNA sequencing. Time course analysis of RNA-Seq data revealed 5608 differentially expressed genes, involved among others in Antigen processing and presentation, Leukocyte transendothelial migration, IL-17 signaling, Chemokine signaling, C-type lectin receptor signaling and Toll-like receptor signaling pathways. In the affected pathways, a number of immune genes were found to be downregulated, suggesting an immune evasion strategy of A. invadans in establishing the infection. The information generated in this study offers first systematic mechanistic understanding of the host-pathogen interaction that might underpin the development of new management strategies for this economically devastating fish-pathogenic oomycete A. invadans.
published_date 2020-11-11T04:13:39Z
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