No Cover Image

Journal article 49 views 16 downloads

Lumbriculus variegatus: A novel organism for in vivo pharmacology education / Aidan Seeley, Caitlin Bellamy, Nia Davies, Lisa Wallace

Pharmacology Research & Perspectives, Volume: 9, Issue: 5

Swansea University Authors: Aidan Seeley, Caitlin Bellamy, Nia Davies, Lisa Wallace

  • 57739.VOR.pdf

    PDF | Version of Record

    This is an open access article under the terms of the Creat ive Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2021 The Authors.

    Download (1.59MB)

Check full text

DOI (Published version): 10.1002/prp2.853

Abstract

Pharmacology graduates require an understanding of both in vitro and in vivo drug responses but there has been a decline in animal use in pharmacology education over the last 30 years. To address this, we present the novel invertebrate model, Lumbriculus variegatus, for in vivo testing of drugs in a...

Full description

Published in: Pharmacology Research & Perspectives
ISSN: 2052-1707 2052-1707
Published: Wiley 2021
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa57739
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: Pharmacology graduates require an understanding of both in vitro and in vivo drug responses but there has been a decline in animal use in pharmacology education over the last 30 years. To address this, we present the novel invertebrate model, Lumbriculus variegatus, for in vivo testing of drugs in a teaching environment. We have developed two novel behavioral assays: the stereotypical movement assay, which measures the effect of drugs on the ability of L. variegatus to perform stereotypical movements following tactile stimulation, and the free locomotion assay, which measures drug effects on unstimulated movement. We report the effects of compounds with diverse pharmacodynamic properties on L. variegatus using these assays. The ryanodine receptor antagonist, dantrolene, altered the unstimulated movement of L. variegatus at 5 μM, whereas stimulated movement was inhibited at ≥25 μM. Lidocaine, a voltage-gated sodium channel blocker, and quinine, a nonselective sodium and potassium channel blocker, reduced both stimulated and unstimulated L. variegatus movement at ≥0.5 mM. Inhibitory effects of quinine persisted for up to 24 h after drug removal, whereas lidocaine effects were reduced 10 min after drug removal. Herein, we provide proof-of-concept utilization of L. variegatus as an organism for use in in vivo pharmacology education but without regulatory constraints or the need for specialized equipment and training.
Keywords: animals, laboratory, education, invertebrates, model, animal models, educational, oligochaeta, teaching
College: Swansea University Medical School
Funders: This work is supporting by an Education Grant from the British Pharmacological Society.
Issue: 5