Journal article 902 views 193 downloads
Lumbriculus variegatus: A novel organism for in vivo pharmacology education
,
Caitlin Bellamy,
Nia Davies,
Lisa Wallace
Pharmacology Research & Perspectives, Volume: 9, Issue: 5
Swansea University Authors:
Aidan Seeley , Caitlin Bellamy, Nia Davies, Lisa Wallace
-
PDF | Version of Record
This is an open access article under the terms of the Creat ive Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2021 The Authors.
Download (1.59MB)
DOI (Published version): 10.1002/prp2.853
Abstract
Pharmacology graduates require an understanding of both in vitro and in vivo drug responses but there has been a decline in animal use in pharmacology education over the last 30 years. To address this, we present the novel invertebrate model, Lumbriculus variegatus, for in vivo testing of drugs in a...
| Published in: | Pharmacology Research & Perspectives |
|---|---|
| ISSN: | 2052-1707 2052-1707 |
| Published: |
Wiley
2021
|
| Online Access: |
Check full text
|
| URI: | https://cronfa.swan.ac.uk/Record/cronfa57739 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract: |
Pharmacology graduates require an understanding of both in vitro and in vivo drug responses but there has been a decline in animal use in pharmacology education over the last 30 years. To address this, we present the novel invertebrate model, Lumbriculus variegatus, for in vivo testing of drugs in a teaching environment. We have developed two novel behavioral assays: the stereotypical movement assay, which measures the effect of drugs on the ability of L. variegatus to perform stereotypical movements following tactile stimulation, and the free locomotion assay, which measures drug effects on unstimulated movement. We report the effects of compounds with diverse pharmacodynamic properties on L. variegatus using these assays. The ryanodine receptor antagonist, dantrolene, altered the unstimulated movement of L. variegatus at 5 μM, whereas stimulated movement was inhibited at ≥25 μM. Lidocaine, a voltage-gated sodium channel blocker, and quinine, a nonselective sodium and potassium channel blocker, reduced both stimulated and unstimulated L. variegatus movement at ≥0.5 mM. Inhibitory effects of quinine persisted for up to 24 h after drug removal, whereas lidocaine effects were reduced 10 min after drug removal. Herein, we provide proof-of-concept utilization of L. variegatus as an organism for use in in vivo pharmacology education but without regulatory constraints or the need for specialized equipment and training. |
|---|---|
| Keywords: |
animals, laboratory, education, invertebrates, model, animal models, educational, oligochaeta, teaching |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This work is supporting by an Education Grant from the British Pharmacological Society. |
| Issue: |
5 |