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Naked-Eye Detection of Hepatitis B Surface Antigen Using Gold Nanoparticles Aggregation and Catalase-Functionalized Polystyrene Nanospheres

Rubing Chen, Yongqin Hu, Meizhu Chen, Jia An, Ying Lyu, Yufei Liu, Dongling Li

ACS Omega, Volume: 6, Issue: 14, Pages: 9828 - 9833

Swansea University Author: Yufei Liu

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Abstract

Developing rapid, efficient, highly sensitive, simple, stable, and low-cost virus marker detection products that are appropriate for basic facilities is of great importance in the early diagnosis and treatment of viruses. Naked-eye detection methods are especially important when medical testing faci...

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Published in: ACS Omega
ISSN: 2470-1343 2470-1343
Published: American Chemical Society (ACS) 2021
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa57787
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Abstract: Developing rapid, efficient, highly sensitive, simple, stable, and low-cost virus marker detection products that are appropriate for basic facilities is of great importance in the early diagnosis and treatment of viruses. Naked-eye detection methods are especially important when medical testing facilities are limited. Polystyrene nanospheres (PSs) with catalytic and specific recognition functions were successfully developed by simultaneously modifying catalase and goat anti-hepatitis B surface antibodies on nanospheres. The modified PSs contributed significantly to the amplification of the signal. Via the specific antigen–antibody reaction, the bifunctional nanospheres could be captured on microplate and then catalyzed the decomposition of hydrogen peroxide to reduce chloroauric acid and synthesize gold nanoparticles (AuNPs). Due to the surface plasmon resonance of AuNPs, the solution color change could be observed with the naked eye and the limit of detection (LOD) was 0.1 ng/mL. Furthermore, the LOD observed with instrumentation was 0.01 ng/mL, which meant that a rapid, efficient, and highly sensitive method for the detection of hepatitis B surface antigens was successfully developed, and neither complex sample pretreatment nor expensive equipment was needed.
Keywords: General Chemical Engineering, General Chemistry
College: College of Engineering
Funders: National Natural Science Foundation of China Grant: 61927818 Identifier: FundRef 10.13039/501100001809 Fundamental Research Funds for the Central Universities of China Grant: 2019CDCGGD304 Grant: 2019CDQYGD020 Identifier: FundRef 10.13039/501100012226 National Key Research and Development Program of China Grant: 2016YFE0125200
Issue: 14
Start Page: 9828
End Page: 9833