No Cover Image

Journal article 30 views 13 downloads

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling

Mirja Tamara Prentzell, Ulrike Rehbein, Marti Cadena Sandoval, Ann-Sofie De Meulemeester, Ralf Baumeister, Laura Brohée, Bianca Berdel, Mathias Bockwoldt, Bernadette Carroll, Suvagata Roy Chowdhury, Andreas von Deimling, Constantinos Demetriades, Gianluca Figlia, Mariana Eca Guimaraes de Araujo, Alexander M. Heberle, Ines Heiland, Birgit Holzwarth, Lukas A. Huber, Jacek Jaworski, Magdalena Kedra, Katharina Kern, Andrii Kopach, Viktor I. Korolchuk, Ineke van 't Land-Kuper, Matylda Macias, Mark Nellist, Wilhelm Palm, Stefan Pusch, Jose Miguel Ramos Pittol, Michèle Reil, Anja Reintjes, Friederike Reuter, Julian R. Sampson, Chloë Scheldeman, Aleksandra Siekierska, Eduard Stefan, Aurelio A. Teleman, Laura Thomas Orcid Logo, Omar Torres-Quesada, Saskia Trump, Hannah D. West, Peter de Witte, Sandra Woltering, Teodor E. Yordanov, Justyna Zmorzynska, Christiane A. Opitz, Kathrin Thedieck

Cell, Volume: 184, Issue: 3, Pages: 655 - 674.e27

Swansea University Author: Laura Thomas Orcid Logo

  • 58991.pdf

    PDF | Version of Record

    2021 The Authors. This is an open access article under the CC BY-NC-ND license

    Download (13.44MB)

Abstract

Ras GTPase-activating protein-binding proteins 1 and 2 (G3BP1 and G3BP2, respectively) are widely recognized as core components of stress granules (SGs). We report that G3BPs reside at the cytoplasmic surface of lysosomes. They act in a non-redundant manner to anchor the tuberous sclerosis complex (...

Full description

Published in: Cell
ISSN: 0092-8674
Published: Elsevier BV 2021
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa58991
Tags: Add Tag
No Tags, Be the first to tag this record!
Abstract: Ras GTPase-activating protein-binding proteins 1 and 2 (G3BP1 and G3BP2, respectively) are widely recognized as core components of stress granules (SGs). We report that G3BPs reside at the cytoplasmic surface of lysosomes. They act in a non-redundant manner to anchor the tuberous sclerosis complex (TSC) protein complex to lysosomes and suppress activation of the metabolic master regulator mechanistic target of rapamycin complex 1 (mTORC1) by amino acids and insulin. Like the TSC complex, G3BP1 deficiency elicits phenotypes related to mTORC1 hyperactivity. In the context of tumors, low G3BP1 levels enhance mTORC1-driven breast cancer cell motility and correlate with adverse outcomes in patients. Furthermore, G3bp1 inhibition in zebrafish disturbs neuronal development and function, leading to white matter heterotopia and neuronal hyperactivity. Thus, G3BPs are not only core components of SGs but also a key element of lysosomal TSC-mTORC1 signaling.
Keywords: TSC complex; mTORC1; G3BP1; G3BP2; lysosome; stress granule; metabolism; cancer; neuronal function
College: Swansea University Medical School
Issue: 3
Start Page: 655
End Page: 674.e27