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Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media

Chedly Tizaoui Orcid Logo, Richard Stanton, Evelina Statkute, Anzelika Rubina, Edward Lester-Card, Anthony Lewis, Peter Holliman Orcid Logo, David Worsley Orcid Logo

Journal of Hazardous Materials, Volume: 428, Start page: 128251

Swansea University Authors: Chedly Tizaoui Orcid Logo, Edward Lester-Card, Anthony Lewis, Peter Holliman Orcid Logo, David Worsley Orcid Logo

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Abstract

This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SA...

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Published in: Journal of Hazardous Materials
ISSN: 0304-3894
Published: Elsevier BV 2022
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa59183
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Abstract: This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19.
Keywords: SARS-CoV-2; Ozone; Disinfection; Sanitisation; Virus
College: College of Engineering
Funders: This work was supported by the Welsh Government Small Business Research Initiative (SBRI) Centre of Excellence - Welsh Ambulance Service & Defence and Security Accelerator, United Kingdom (ACC2014616).
Start Page: 128251