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Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media

Chedly Tizaoui Orcid Logo, Richard Stanton, Evelina Statkute, Anzelika Rubina, Edward Lester-Card, Anthony Lewis, Peter Holliman Orcid Logo, David Worsley Orcid Logo

Journal of Hazardous Materials, Volume: 428, Start page: 128251

Swansea University Authors: Chedly Tizaoui Orcid Logo, Edward Lester-Card, Anthony Lewis, Peter Holliman Orcid Logo, David Worsley Orcid Logo

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Abstract

This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SA...

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Published in: Journal of Hazardous Materials
ISSN: 0304-3894
Published: Elsevier BV 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa59183
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Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 &#xB1; 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. 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spelling 2022-05-26T13:04:19.0508799 v2 59183 2022-01-14 Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media 4b34a0286d3c0b0b081518fa6987031d 0000-0003-2159-7881 Chedly Tizaoui Chedly Tizaoui true false 021c5bbf5c153fa16bcbbb8514e35869 Edward Lester-Card Edward Lester-Card true false 328c21711ee3091505363e2b5060fba0 Anthony Lewis Anthony Lewis true false c8f52394d776279c9c690dc26066ddf9 0000-0002-9911-8513 Peter Holliman Peter Holliman true false c426b1c1b0123d7057c1b969083cea69 0000-0002-9956-6228 David Worsley David Worsley true false 2022-01-14 CHEG This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19. Journal Article Journal of Hazardous Materials 428 128251 Elsevier BV 0304-3894 SARS-CoV-2; Ozone; Disinfection; Sanitisation; Virus 15 4 2022 2022-04-15 10.1016/j.jhazmat.2022.128251 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University This work was supported by the Welsh Government Small Business Research Initiative (SBRI) Centre of Excellence - Welsh Ambulance Service & Defence and Security Accelerator, United Kingdom (ACC2014616). 2022-05-26T13:04:19.0508799 2022-01-14T19:07:53.6409170 College of Engineering Engineering Chedly Tizaoui 0000-0003-2159-7881 1 Richard Stanton 2 Evelina Statkute 3 Anzelika Rubina 4 Edward Lester-Card 5 Anthony Lewis 6 Peter Holliman 0000-0002-9911-8513 7 David Worsley 0000-0002-9956-6228 8 59183__22305__a2f17dffd280430a9816f922c892b102.pdf 59183.pdf 2022-02-04T14:42:01.4572672 Output 2235941 application/pdf Version of Record true © 2022 The Authors. This is an open access article under the CC BY license true eng http://creativecommons.org/licenses/by/4.0/
title Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
spellingShingle Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
Chedly Tizaoui
Edward Lester-Card
Anthony Lewis
Peter Holliman
David Worsley
title_short Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_full Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_fullStr Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_full_unstemmed Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_sort Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
author_id_str_mv 4b34a0286d3c0b0b081518fa6987031d
021c5bbf5c153fa16bcbbb8514e35869
328c21711ee3091505363e2b5060fba0
c8f52394d776279c9c690dc26066ddf9
c426b1c1b0123d7057c1b969083cea69
author_id_fullname_str_mv 4b34a0286d3c0b0b081518fa6987031d_***_Chedly Tizaoui
021c5bbf5c153fa16bcbbb8514e35869_***_Edward Lester-Card
328c21711ee3091505363e2b5060fba0_***_Anthony Lewis
c8f52394d776279c9c690dc26066ddf9_***_Peter Holliman
c426b1c1b0123d7057c1b969083cea69_***_David Worsley
author Chedly Tizaoui
Edward Lester-Card
Anthony Lewis
Peter Holliman
David Worsley
author2 Chedly Tizaoui
Richard Stanton
Evelina Statkute
Anzelika Rubina
Edward Lester-Card
Anthony Lewis
Peter Holliman
David Worsley
format Journal article
container_title Journal of Hazardous Materials
container_volume 428
container_start_page 128251
publishDate 2022
institution Swansea University
issn 0304-3894
doi_str_mv 10.1016/j.jhazmat.2022.128251
publisher Elsevier BV
college_str College of Engineering
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hierarchy_top_id collegeofengineering
hierarchy_top_title College of Engineering
hierarchy_parent_id collegeofengineering
hierarchy_parent_title College of Engineering
department_str Engineering{{{_:::_}}}College of Engineering{{{_:::_}}}Engineering
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description This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19.
published_date 2022-04-15T04:16:17Z
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