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Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.

Emma Quinn, Sophie Malkin, Jessica E. Thomas, RYAN POOLE, Charlotte Davies Orcid Logo, Andrew Rowley, Christopher Coates

Biological Invasions, Volume: 24, Pages: 3647 - 3659

Swansea University Authors: Emma Quinn, Sophie Malkin, RYAN POOLE, Charlotte Davies Orcid Logo, Andrew Rowley, Christopher Coates

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DOI (Published version): 10.1007/s10530-022-02868-6

Abstract

A large knowledge gap exists regarding the disease profile and pathologic condition of the invasive, slipper limpet Crepidula fornicata. To help address this, we performed a year-long health survey at two sites in South Wales, UK – subtidal Swansea Bay and intertidal Milford Haven. In total, 1,800 l...

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Published in: Biological Invasions
ISSN: 1387-3547 1573-1464
Published: Springer Science and Business Media LLC 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa60643
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To help address this, we performed a year-long health survey at two sites in South Wales, UK &#x2013; subtidal Swansea Bay and intertidal Milford Haven. In total, 1,800 limpets were screened systematically for haemolymph bacterial burdens using both general and vibrio-selective growth media (TSA&#x2009;+&#x2009;2% NaCl and TCBS, respectively), haemolymph (blood) inspection using microscopy, a PCR-based assay targeting Vibrio spp., and multi-tissue histology. Over 99% of haemolymph samples contained cultivable bacterial colony-forming units, and 83% of limpets tested positive for the presence of vibrios via PCR (confirmed via Sanger sequencing). Vibrio presence did not vary greatly between sites, yet a strong seasonal effect was observed with significantly higher bacterial loads during the summer. Binomial logistic regression models revealed larger (older) limpets were more likely to harbour vibrios, and the growth of bacteria on TCBS was a key predictor for PCR-based vibrio detection. Histological assessment of &gt;&#x2009;340 animals revealed little evidence of inflammation, sepsis or immune reactivity despite the gross bacterial numbers. We contend that slipper limpets are not highly susceptible to bacteriosis at either site, and do not harbour vibrios known to be pathogenic to humans. The lack of susceptibility to local pathogenic bacteria may explain, in part, the invasion success of C. fornicata across this region.</abstract><type>Journal Article</type><journal>Biological Invasions</journal><volume>24</volume><journalNumber/><paginationStart>3647</paginationStart><paginationEnd>3659</paginationEnd><publisher>Springer Science and Business Media LLC</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>1387-3547</issnPrint><issnElectronic>1573-1464</issnElectronic><keywords>Bacteriosis, disease connectivity, oyster pest; Haemocytes; Epizootiology; Aquaculture &amp;amp; fisheries; Splendidus clade</keywords><publishedDay>20</publishedDay><publishedMonth>7</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-07-20</publishedDate><doi>10.1007/s10530-022-02868-6</doi><url/><notes>Data Availability:Sequence data have been deposited into NCBI&#x2019;s short read archive (SRA) under accession numbers SRR13165025 &#x2013; SRR13165046. 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spelling 2023-01-04T15:25:42.1129893 v2 60643 2022-07-27 Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp. 6cb18d2ebc7cfec175374f0ff559d06b Emma Quinn Emma Quinn true false 17ff4136248e9382802ced1bc1d680e4 Sophie Malkin Sophie Malkin true false a3180edafbe58070c6a23d11da0f3e0b RYAN POOLE RYAN POOLE true false a0febe211e502356dad1dab51a43761c 0000-0002-5853-1934 Charlotte Davies Charlotte Davies true false e98124f6e62b9592786899d7059e3a79 Andrew Rowley Andrew Rowley true false af160934b75bea5b8ba83d68b3d1a003 Christopher Coates Christopher Coates true false 2022-07-27 SBI A large knowledge gap exists regarding the disease profile and pathologic condition of the invasive, slipper limpet Crepidula fornicata. To help address this, we performed a year-long health survey at two sites in South Wales, UK – subtidal Swansea Bay and intertidal Milford Haven. In total, 1,800 limpets were screened systematically for haemolymph bacterial burdens using both general and vibrio-selective growth media (TSA + 2% NaCl and TCBS, respectively), haemolymph (blood) inspection using microscopy, a PCR-based assay targeting Vibrio spp., and multi-tissue histology. Over 99% of haemolymph samples contained cultivable bacterial colony-forming units, and 83% of limpets tested positive for the presence of vibrios via PCR (confirmed via Sanger sequencing). Vibrio presence did not vary greatly between sites, yet a strong seasonal effect was observed with significantly higher bacterial loads during the summer. Binomial logistic regression models revealed larger (older) limpets were more likely to harbour vibrios, and the growth of bacteria on TCBS was a key predictor for PCR-based vibrio detection. Histological assessment of > 340 animals revealed little evidence of inflammation, sepsis or immune reactivity despite the gross bacterial numbers. We contend that slipper limpets are not highly susceptible to bacteriosis at either site, and do not harbour vibrios known to be pathogenic to humans. The lack of susceptibility to local pathogenic bacteria may explain, in part, the invasion success of C. fornicata across this region. Journal Article Biological Invasions 24 3647 3659 Springer Science and Business Media LLC 1387-3547 1573-1464 Bacteriosis, disease connectivity, oyster pest; Haemocytes; Epizootiology; Aquaculture &amp; fisheries; Splendidus clade 20 7 2022 2022-07-20 10.1007/s10530-022-02868-6 Data Availability:Sequence data have been deposited into NCBI’s short read archive (SRA) under accession numbers SRR13165025 – SRR13165046. Data for limpet morphometrics, haemocyte numbers, bacterial colony-forming units and PCR-based screening have been included as supplementary material. COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University SU Library paid the OA fee (TA Institutional Deal) Operations were part funded by the European Regional Development Fund through the Ireland-Wales Cooperation programme, BLUEFISH, awarded to CC and AR, and Swansea University start-up funds assigned to CC. A BLUEFISH innovation bursary and a College of Science (Swansea University) doctoral training grant supported EQ. 2023-01-04T15:25:42.1129893 2022-07-27T11:14:06.9579386 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Emma Quinn 1 Sophie Malkin 2 Jessica E. Thomas 3 RYAN POOLE 4 Charlotte Davies 0000-0002-5853-1934 5 Andrew Rowley 6 Christopher Coates 7 60643__24860__8e282726d7f04d13a5f0b2ba7c8418fa.pdf 60643.pdf 2022-08-05T12:04:50.1775080 Output 2786476 application/pdf Version of Record true © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/
title Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.
spellingShingle Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.
Emma Quinn
Sophie Malkin
RYAN POOLE
Charlotte Davies
Andrew Rowley
Christopher Coates
title_short Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.
title_full Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.
title_fullStr Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.
title_full_unstemmed Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.
title_sort Invasive slipper limpets (Crepidula fornicata) act like a sink, rather than source, of Vibrio spp.
author_id_str_mv 6cb18d2ebc7cfec175374f0ff559d06b
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a0febe211e502356dad1dab51a43761c
e98124f6e62b9592786899d7059e3a79
af160934b75bea5b8ba83d68b3d1a003
author_id_fullname_str_mv 6cb18d2ebc7cfec175374f0ff559d06b_***_Emma Quinn
17ff4136248e9382802ced1bc1d680e4_***_Sophie Malkin
a3180edafbe58070c6a23d11da0f3e0b_***_RYAN POOLE
a0febe211e502356dad1dab51a43761c_***_Charlotte Davies
e98124f6e62b9592786899d7059e3a79_***_Andrew Rowley
af160934b75bea5b8ba83d68b3d1a003_***_Christopher Coates
author Emma Quinn
Sophie Malkin
RYAN POOLE
Charlotte Davies
Andrew Rowley
Christopher Coates
author2 Emma Quinn
Sophie Malkin
Jessica E. Thomas
RYAN POOLE
Charlotte Davies
Andrew Rowley
Christopher Coates
format Journal article
container_title Biological Invasions
container_volume 24
container_start_page 3647
publishDate 2022
institution Swansea University
issn 1387-3547
1573-1464
doi_str_mv 10.1007/s10530-022-02868-6
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description A large knowledge gap exists regarding the disease profile and pathologic condition of the invasive, slipper limpet Crepidula fornicata. To help address this, we performed a year-long health survey at two sites in South Wales, UK – subtidal Swansea Bay and intertidal Milford Haven. In total, 1,800 limpets were screened systematically for haemolymph bacterial burdens using both general and vibrio-selective growth media (TSA + 2% NaCl and TCBS, respectively), haemolymph (blood) inspection using microscopy, a PCR-based assay targeting Vibrio spp., and multi-tissue histology. Over 99% of haemolymph samples contained cultivable bacterial colony-forming units, and 83% of limpets tested positive for the presence of vibrios via PCR (confirmed via Sanger sequencing). Vibrio presence did not vary greatly between sites, yet a strong seasonal effect was observed with significantly higher bacterial loads during the summer. Binomial logistic regression models revealed larger (older) limpets were more likely to harbour vibrios, and the growth of bacteria on TCBS was a key predictor for PCR-based vibrio detection. Histological assessment of > 340 animals revealed little evidence of inflammation, sepsis or immune reactivity despite the gross bacterial numbers. We contend that slipper limpets are not highly susceptible to bacteriosis at either site, and do not harbour vibrios known to be pathogenic to humans. The lack of susceptibility to local pathogenic bacteria may explain, in part, the invasion success of C. fornicata across this region.
published_date 2022-07-20T04:18:55Z
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