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Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

Alison Dickson, Eylan Yutuc Orcid Logo, Cathy Thornton Orcid Logo, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

Frontiers in Endocrinology, Volume: 13

Swansea University Authors: Alison Dickson, Eylan Yutuc Orcid Logo, Cathy Thornton Orcid Logo, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

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Abstract

The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human...

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Published in: Frontiers in Endocrinology
ISSN: 1664-2392
Published: Frontiers Media SA 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa61630
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Abstract: The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.
Item Description: Research data via DOI 10.17605/OSF.IO/EGNCZ
Keywords: sterol, oxysterol, bile acids, CYP11A1, LC-MS, CYP27A1
College: Faculty of Medicine, Health and Life Sciences
Funders: This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and BB/S019588/1 to WJG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic Expertise for Business project (to WJG and YW). ALD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund.