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Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
Frontiers in Endocrinology, Volume: 13
Swansea University Authors: Alison Dickson, Eylan Yutuc , Cathy Thornton , Yuqin Wang , William Griffiths
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© 2022 Dickson, Yutuc, Thornton, Wang and Griffiths. This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY)Download (13.98MB)
DOI (Published version): 10.3389/fendo.2022.1031013
The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human...
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The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.
Research data via DOI 10.17605/OSF.IO/EGNCZ
sterol, oxysterol, bile acids, CYP11A1, LC-MS, CYP27A1
Faculty of Medicine, Health and Life Sciences
This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and
BB/S019588/1 to WJG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic
Expertise for Business project (to WJG and YW). ALD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund.