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Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells

Mackenzie J. Dickson, Martin Sheldon Orcid Logo, John J. Bromfield Orcid Logo

CABI Agriculture and Bioscience, Volume: 3, Issue: 1, Pages: 1 - 15

Swansea University Author: Martin Sheldon Orcid Logo

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DOI (Published version): 10.1186/s43170-022-00133-3

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BackgroundBacterial infection of the uterus in postpartum dairy cows limits ovarian follicle growth, reduces blood estradiol concentrations, and leads to accumulation of bacterial lipopolysaccharide (LPS) in ovarian follicular fluid. Although treating granulosa cells with LPS in vitro decreases the...

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Published in: CABI Agriculture and Bioscience
ISSN: 2662-4044
Published: Springer Science and Business Media LLC 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa61648
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2022-11-14T10:52:50.4128056</datestamp><bib-version>v2</bib-version><id>61648</id><entry>2022-10-24</entry><title>Lipopolysaccharide alters CEBP&#x3B2; signaling and reduces estradiol production in bovine granulosa cells</title><swanseaauthors><author><sid>ab0f74b794e59cc270c69e63ee1d9748</sid><ORCID>0000-0001-7902-5558</ORCID><firstname>Martin</firstname><surname>Sheldon</surname><name>Martin Sheldon</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2022-10-24</date><deptcode>BMS</deptcode><abstract>BackgroundBacterial infection of the uterus in postpartum dairy cows limits ovarian follicle growth, reduces blood estradiol concentrations, and leads to accumulation of bacterial lipopolysaccharide (LPS) in ovarian follicular fluid. Although treating granulosa cells with LPS in vitro decreases the expression of the estradiol synthesis enzyme CYP19A1 and reduces estradiol secretion, the molecular mechanisms are unclear. The transcription factor CCAAT enhancer binding protein beta (CEBP&#x3B2;) not only facilitates the transcription of LPS regulated cytokines, but also binds to the promoter region of CYP19A1 in humans, mice, and buffalo. We hypothesized that LPS alters CEBP&#x3B2; signaling to reduce CYP19A1 expression, resulting in decreased estradiol secretion.MethodsBovine granulosa cells were isolated from small/medium or large follicles and treated with LPS in the presence of FSH and androstenedione for up to 24 h.ResultsTreatment with LPS increased CXCL8 and IL6 gene expression and reduced estradiol secretion in granulosa cells from both small/medium and large follicles. However, LPS only reduced CYP19A1 expression in granulosa cells from large follicles. Treatment with LPS increased CEBPB expression and reduced CEBP&#x3B2; nuclear localization in granulosa cells from small/medium follicles, but not granulosa cells from large follicles.ConclusionsAlthough LPS reduces estradiol synthesis in bovine granulosa cells, the effects of LPS on CYP19A1 and CEBP&#x3B2; are dependent on follicle size.</abstract><type>Journal Article</type><journal>CABI Agriculture and Bioscience</journal><volume>3</volume><journalNumber>1</journalNumber><paginationStart>1</paginationStart><paginationEnd>15</paginationEnd><publisher>Springer Science and Business Media LLC</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2662-4044</issnElectronic><keywords>Granulosa cell; Estradiol; Lipopolysaccharide; Inflammation</keywords><publishedDay>22</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-10-22</publishedDate><doi>10.1186/s43170-022-00133-3</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders>This study was supported by Eunice Kennedy Shriver National Institute of Child Health &amp; Human Development of the National Institutes of Health under Award Number R01HD084316 and by Agriculture and Food Research Initiative Competitive Grant No. 2020-67015-31015 from the USDA National Institute of Food and Agriculture.</funders><projectreference/><lastEdited>2022-11-14T10:52:50.4128056</lastEdited><Created>2022-10-24T15:36:57.8545446</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Mackenzie J.</firstname><surname>Dickson</surname><order>1</order></author><author><firstname>Martin</firstname><surname>Sheldon</surname><orcid>0000-0001-7902-5558</orcid><order>2</order></author><author><firstname>John J.</firstname><surname>Bromfield</surname><orcid>0000-0001-5438-2137</orcid><order>3</order></author></authors><documents><document><filename>61648__25567__bff7fd81480c4a0db33947a683707176.pdf</filename><originalFilename>s43170-022-00133-3.pdf</originalFilename><uploaded>2022-10-24T15:40:19.9905599</uploaded><type>Output</type><contentLength>3502421</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2022-11-14T10:52:50.4128056 v2 61648 2022-10-24 Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2022-10-24 BMS BackgroundBacterial infection of the uterus in postpartum dairy cows limits ovarian follicle growth, reduces blood estradiol concentrations, and leads to accumulation of bacterial lipopolysaccharide (LPS) in ovarian follicular fluid. Although treating granulosa cells with LPS in vitro decreases the expression of the estradiol synthesis enzyme CYP19A1 and reduces estradiol secretion, the molecular mechanisms are unclear. The transcription factor CCAAT enhancer binding protein beta (CEBPβ) not only facilitates the transcription of LPS regulated cytokines, but also binds to the promoter region of CYP19A1 in humans, mice, and buffalo. We hypothesized that LPS alters CEBPβ signaling to reduce CYP19A1 expression, resulting in decreased estradiol secretion.MethodsBovine granulosa cells were isolated from small/medium or large follicles and treated with LPS in the presence of FSH and androstenedione for up to 24 h.ResultsTreatment with LPS increased CXCL8 and IL6 gene expression and reduced estradiol secretion in granulosa cells from both small/medium and large follicles. However, LPS only reduced CYP19A1 expression in granulosa cells from large follicles. Treatment with LPS increased CEBPB expression and reduced CEBPβ nuclear localization in granulosa cells from small/medium follicles, but not granulosa cells from large follicles.ConclusionsAlthough LPS reduces estradiol synthesis in bovine granulosa cells, the effects of LPS on CYP19A1 and CEBPβ are dependent on follicle size. Journal Article CABI Agriculture and Bioscience 3 1 1 15 Springer Science and Business Media LLC 2662-4044 Granulosa cell; Estradiol; Lipopolysaccharide; Inflammation 22 10 2022 2022-10-22 10.1186/s43170-022-00133-3 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Another institution paid the OA fee This study was supported by Eunice Kennedy Shriver National Institute of Child Health & Human Development of the National Institutes of Health under Award Number R01HD084316 and by Agriculture and Food Research Initiative Competitive Grant No. 2020-67015-31015 from the USDA National Institute of Food and Agriculture. 2022-11-14T10:52:50.4128056 2022-10-24T15:36:57.8545446 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Mackenzie J. Dickson 1 Martin Sheldon 0000-0001-7902-5558 2 John J. Bromfield 0000-0001-5438-2137 3 61648__25567__bff7fd81480c4a0db33947a683707176.pdf s43170-022-00133-3.pdf 2022-10-24T15:40:19.9905599 Output 3502421 application/pdf Version of Record true © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/
title Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
spellingShingle Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
Martin Sheldon
title_short Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
title_full Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
title_fullStr Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
title_full_unstemmed Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
title_sort Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells
author_id_str_mv ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author Martin Sheldon
author2 Mackenzie J. Dickson
Martin Sheldon
John J. Bromfield
format Journal article
container_title CABI Agriculture and Bioscience
container_volume 3
container_issue 1
container_start_page 1
publishDate 2022
institution Swansea University
issn 2662-4044
doi_str_mv 10.1186/s43170-022-00133-3
publisher Springer Science and Business Media LLC
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description BackgroundBacterial infection of the uterus in postpartum dairy cows limits ovarian follicle growth, reduces blood estradiol concentrations, and leads to accumulation of bacterial lipopolysaccharide (LPS) in ovarian follicular fluid. Although treating granulosa cells with LPS in vitro decreases the expression of the estradiol synthesis enzyme CYP19A1 and reduces estradiol secretion, the molecular mechanisms are unclear. The transcription factor CCAAT enhancer binding protein beta (CEBPβ) not only facilitates the transcription of LPS regulated cytokines, but also binds to the promoter region of CYP19A1 in humans, mice, and buffalo. We hypothesized that LPS alters CEBPβ signaling to reduce CYP19A1 expression, resulting in decreased estradiol secretion.MethodsBovine granulosa cells were isolated from small/medium or large follicles and treated with LPS in the presence of FSH and androstenedione for up to 24 h.ResultsTreatment with LPS increased CXCL8 and IL6 gene expression and reduced estradiol secretion in granulosa cells from both small/medium and large follicles. However, LPS only reduced CYP19A1 expression in granulosa cells from large follicles. Treatment with LPS increased CEBPB expression and reduced CEBPβ nuclear localization in granulosa cells from small/medium follicles, but not granulosa cells from large follicles.ConclusionsAlthough LPS reduces estradiol synthesis in bovine granulosa cells, the effects of LPS on CYP19A1 and CEBPβ are dependent on follicle size.
published_date 2022-10-22T04:20:37Z
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