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Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen
Hernia, Volume: 24, Issue: 6, Pages: 1175 - 1189
Swansea University Author: Luke Davies
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DOI (Published version): 10.1007/s10029-020-02315-2
Abstract
BackgroundMesh implants are widely used to reinforce the abdominal wall, although the inevitable inflammatory foreign body reaction (FBR) at the interface leads to complications. Macrophages are suspected to regulate the subsequent scar formation, but it is still unclear whether adequate fibrous sca...
Published in: | Hernia |
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ISSN: | 1265-4906 1248-9204 |
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Springer Science and Business Media LLC
2020
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URI: | https://cronfa.swan.ac.uk/Record/cronfa61680 |
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2022-11-07T15:42:27.4263606 v2 61680 2022-10-31 Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen ff080296775381560053d5e3a6e81745 0000-0001-7767-4060 Luke Davies Luke Davies true false 2022-10-31 BMS BackgroundMesh implants are widely used to reinforce the abdominal wall, although the inevitable inflammatory foreign body reaction (FBR) at the interface leads to complications. Macrophages are suspected to regulate the subsequent scar formation, but it is still unclear whether adequate fibrous scar formation with collagen deposition depends mainly on the presence of M1 or M2 macrophages.MethodsThis study investigated the FBR to seven human polypropylene meshes, which were removed after a median incorporation time of 1 year due to the primary complaint of recurrence. Using immunofluorescence, the FBR was examined in six regional zones with increasing distance from the mesh fibers up to 350 µm, based on the cell densities, macrophage M1 (CD86) and M2 (CD163, CD206) phenotypes, deposition of collagen-I and -III, and expression of matrix metalloproteinase-2 (MMP-2) and -8 as indicator of collagen degradation.ResultsAll mesh–tissue complexes demonstrated a decrease in cell density and macrophages with distance to the mesh fibers. Overall, about 60% of the macrophages presented an M2 phenotype, whereas only 6% an M1 phenotype. Over 70% of macrophages showed co-expression with collagen-I or -III and over 50% with MMP-2.ConclusionsThe chronic FBR to polypropylene meshes is associated with an M2 macrophage response, which is accompanied by collagen deposition and MMP-2 expression. These findings challenge the idea that mainly M1 macrophages are related to inflammation and highlights that iatrogenic attempts to polarize these cells towards the M2 phenotype may not be a solution to ameliorate the long-term foreign body reaction. Journal Article Hernia 24 6 1175 1189 Springer Science and Business Media LLC 1265-4906 1248-9204 Foreign body reaction; Macrophage; Collagen; Mesh; Fluorescence microscopy 1 12 2020 2020-12-01 10.1007/s10029-020-02315-2 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Open Access funding enabled and organized by Projekt DEAL.. Open Access funding enabled and organized by Projekt DEAL. The support by the Federal Ministry of Education and Research (FKZ 13GW0108B) enabled the acquisition of the TissueGnostics system. 2022-11-07T15:42:27.4263606 2022-10-31T11:58:32.6719653 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine A. Dievernich 0000-0001-6677-9801 1 P. Achenbach 2 Luke Davies 0000-0001-7767-4060 3 U. Klinge 4 61680__25682__4ae09a3e017f464981b2b81b8e298aa0.pdf 61680.pdf 2022-11-07T15:40:50.5658671 Output 4412539 application/pdf Version of Record true © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/ |
title |
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen |
spellingShingle |
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen Luke Davies |
title_short |
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen |
title_full |
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen |
title_fullStr |
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen |
title_full_unstemmed |
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen |
title_sort |
Tissue remodeling macrophages morphologically dominate at the interface of polypropylene surgical meshes in the human abdomen |
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ff080296775381560053d5e3a6e81745 |
author_id_fullname_str_mv |
ff080296775381560053d5e3a6e81745_***_Luke Davies |
author |
Luke Davies |
author2 |
A. Dievernich P. Achenbach Luke Davies U. Klinge |
format |
Journal article |
container_title |
Hernia |
container_volume |
24 |
container_issue |
6 |
container_start_page |
1175 |
publishDate |
2020 |
institution |
Swansea University |
issn |
1265-4906 1248-9204 |
doi_str_mv |
10.1007/s10029-020-02315-2 |
publisher |
Springer Science and Business Media LLC |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
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Faculty of Medicine, Health and Life Sciences |
department_str |
Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine |
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description |
BackgroundMesh implants are widely used to reinforce the abdominal wall, although the inevitable inflammatory foreign body reaction (FBR) at the interface leads to complications. Macrophages are suspected to regulate the subsequent scar formation, but it is still unclear whether adequate fibrous scar formation with collagen deposition depends mainly on the presence of M1 or M2 macrophages.MethodsThis study investigated the FBR to seven human polypropylene meshes, which were removed after a median incorporation time of 1 year due to the primary complaint of recurrence. Using immunofluorescence, the FBR was examined in six regional zones with increasing distance from the mesh fibers up to 350 µm, based on the cell densities, macrophage M1 (CD86) and M2 (CD163, CD206) phenotypes, deposition of collagen-I and -III, and expression of matrix metalloproteinase-2 (MMP-2) and -8 as indicator of collagen degradation.ResultsAll mesh–tissue complexes demonstrated a decrease in cell density and macrophages with distance to the mesh fibers. Overall, about 60% of the macrophages presented an M2 phenotype, whereas only 6% an M1 phenotype. Over 70% of macrophages showed co-expression with collagen-I or -III and over 50% with MMP-2.ConclusionsThe chronic FBR to polypropylene meshes is associated with an M2 macrophage response, which is accompanied by collagen deposition and MMP-2 expression. These findings challenge the idea that mainly M1 macrophages are related to inflammation and highlights that iatrogenic attempts to polarize these cells towards the M2 phenotype may not be a solution to ameliorate the long-term foreign body reaction. |
published_date |
2020-12-01T04:20:40Z |
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11.030209 |