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CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining

Uwe Klinge, Axel Dievernich, Rene Tolba, Bernd Klosterhalfen, Luke Davies Orcid Logo

Journal of Biomedical Materials Research Part B: Applied Biomaterials, Volume: 108, Issue: 8, Pages: 3134 - 3146

Swansea University Author: Luke Davies Orcid Logo

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DOI (Published version): 10.1002/jbm.b.34639

Abstract

Implants like meshes for the reinforcement of tissues implement the formation of a persistent inflammation with an ambient fibrotic reaction. In the inflammatory infiltrate several distinct cell types have been identified, but CD68+ macrophages are supposed to be most important. To investigate the c...

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Published in: Journal of Biomedical Materials Research Part B: Applied Biomaterials
ISSN: 1552-4973 1552-4981
Published: Wiley 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa61684
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spelling 2022-11-07T15:32:08.1468228 v2 61684 2022-10-31 CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining ff080296775381560053d5e3a6e81745 0000-0001-7767-4060 Luke Davies Luke Davies true false 2022-10-31 BMS Implants like meshes for the reinforcement of tissues implement the formation of a persistent inflammation with an ambient fibrotic reaction. In the inflammatory infiltrate several distinct cell types have been identified, but CD68+ macrophages are supposed to be most important. To investigate the collaboration among the various cell types within the infiltrate we performed at explanted meshes from humans double fluorescence staining with CD68 as a constant marker and a variety of other antibodies as the second marker. The list of second markers includes lymphocytes (CD3, CD4, CD8, CD16, CD56, FoxP3, and CD11b) stem cells (CD34), leucocytes (CD45, CD15), macrophages (CD86, CD105, CD163, and CD206); deposition of EC matrix (collagen-I, collagen-III, MMP2, and MMP8); Ki67 as a marker for proliferation; and the tyrosine-protein kinase receptor AXL. The present study demonstrates within the inflammatory infiltrate the abundant capability of CD68+ cells to co-express a huge variety of other markers, including those of lymphocytes, varying between 5 and 83% of investigated cells. The observation of co-staining was not restricted to a specific polymer but was seen with polypropylene fibers as well as with fibers made of polyvinylidene fluoride, although with differences in co-expression rates. The persisting variability of these cells without the functional reduction toward differentiated mature cell types may favor the lack of healing at the interface of meshes. Journal Article Journal of Biomedical Materials Research Part B: Applied Biomaterials 108 8 3134 3146 Wiley 1552-4973 1552-4981 fluorescence microscopy; foreign body reaction; lymphocyte; macrophage; mesh 1 11 2020 2020-11-01 10.1002/jbm.b.34639 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Federal Ministry of Education and Research, Germany. Grant Number: FKZ 13GW0108B; Welcome Trust. Grant Number: 103973/Z/14/Z 2022-11-07T15:32:08.1468228 2022-10-31T12:01:06.6605376 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Uwe Klinge 1 Axel Dievernich 2 Rene Tolba 3 Bernd Klosterhalfen 4 Luke Davies 0000-0001-7767-4060 5 61684__25680__f4cec32f34af4e4c83eea8ad356ad308.pdf 61684.pdf 2022-11-07T15:31:50.6446379 Output 20759220 application/pdf Version of Record true © 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, true eng http://creativecommons.org/licenses/by/4.0/
title CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
spellingShingle CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
Luke Davies
title_short CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
title_full CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
title_fullStr CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
title_full_unstemmed CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
title_sort CD68+ macrophages as crucial components of the foreign body reaction demonstrate an unconventional pattern of functional markers quantified by analysis with double fluorescence staining
author_id_str_mv ff080296775381560053d5e3a6e81745
author_id_fullname_str_mv ff080296775381560053d5e3a6e81745_***_Luke Davies
author Luke Davies
author2 Uwe Klinge
Axel Dievernich
Rene Tolba
Bernd Klosterhalfen
Luke Davies
format Journal article
container_title Journal of Biomedical Materials Research Part B: Applied Biomaterials
container_volume 108
container_issue 8
container_start_page 3134
publishDate 2020
institution Swansea University
issn 1552-4973
1552-4981
doi_str_mv 10.1002/jbm.b.34639
publisher Wiley
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Implants like meshes for the reinforcement of tissues implement the formation of a persistent inflammation with an ambient fibrotic reaction. In the inflammatory infiltrate several distinct cell types have been identified, but CD68+ macrophages are supposed to be most important. To investigate the collaboration among the various cell types within the infiltrate we performed at explanted meshes from humans double fluorescence staining with CD68 as a constant marker and a variety of other antibodies as the second marker. The list of second markers includes lymphocytes (CD3, CD4, CD8, CD16, CD56, FoxP3, and CD11b) stem cells (CD34), leucocytes (CD45, CD15), macrophages (CD86, CD105, CD163, and CD206); deposition of EC matrix (collagen-I, collagen-III, MMP2, and MMP8); Ki67 as a marker for proliferation; and the tyrosine-protein kinase receptor AXL. The present study demonstrates within the inflammatory infiltrate the abundant capability of CD68+ cells to co-express a huge variety of other markers, including those of lymphocytes, varying between 5 and 83% of investigated cells. The observation of co-staining was not restricted to a specific polymer but was seen with polypropylene fibers as well as with fibers made of polyvinylidene fluoride, although with differences in co-expression rates. The persisting variability of these cells without the functional reduction toward differentiated mature cell types may favor the lack of healing at the interface of meshes.
published_date 2020-11-01T04:20:41Z
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