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Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen

A. Dievernich Orcid Logo, P. Achenbach, Luke Davies Orcid Logo, U. Klinge

Hernia, Volume: 26, Issue: 1, Pages: 309 - 323

Swansea University Author: Luke Davies Orcid Logo

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DOI (Published version): 10.1007/s10029-021-02396-7

Abstract

BackgroundPolypropylene (PP) mesh is widely used to reinforce tissues. The foreign body reaction (FBR) to the implant is dominated by innate immune cells, especially macrophages. However, considerable numbers of adaptive immune cells, namely T cells, have also been regularly observed, which appear t...

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Published in: Hernia
ISSN: 1265-4906 1248-9204
Published: Springer Science and Business Media LLC 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa61693
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2022-11-07T14:56:09.7458679</datestamp><bib-version>v2</bib-version><id>61693</id><entry>2022-10-31</entry><title>Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen</title><swanseaauthors><author><sid>ff080296775381560053d5e3a6e81745</sid><ORCID>0000-0001-7767-4060</ORCID><firstname>Luke</firstname><surname>Davies</surname><name>Luke Davies</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2022-10-31</date><deptcode>BMS</deptcode><abstract>BackgroundPolypropylene (PP) mesh is widely used to reinforce tissues. The foreign body reaction (FBR) to the implant is dominated by innate immune cells, especially macrophages. However, considerable numbers of adaptive immune cells, namely T cells, have also been regularly observed, which appear to play a crucial role in the long-term host response.MethodsThis study investigated the FBR to seven human PP meshes, which were removed from the abdomen for recurrence after a median of one year. Using immunofluorescence microscopy, the FBR was examined for various innate (CD11b+ myeloid, CD68+ macrophages, CD56+ NK) and adaptive immune cells (CD3+ T, CD4+ T-helper, CD8+ cytotoxic, FoxP3+ T-regulatory, CD20+ B) as well as &#x201C;conventional&#x201D; immune cells (defined as cells expressing their specific immune cell marker without co-expressing CD68).ResultsT-helper cells (19%) and regulatory T-cells (25%) were present at comparable rates to macrophages, and clustered significantly toward the mesh fibers. For all cell types the lowest proportions of &#x201C;conventional&#x201D; cells (&lt;&#x2009;60%) were observed at the mesh&#x2013;tissue interface, but increased considerably at about 50&#x2013;100 &#xB5;m, indicating reduced stimulation with rising distance to the mesh fibers.ConclusionBoth innate and adaptive immune cells participate in the chronic FBR to PP meshes with T cells and macrophages being the predominant cell types, respectively. In concordance with the previous data, many cells presented a &#x201C;hybrid&#x201D; pattern near the mesh fibers. The complexity of the immune reaction seen within the foreign body granuloma may explain why approaches focusing on specific cell types have not been very successful in reducing the chronic FBR.</abstract><type>Journal Article</type><journal>Hernia</journal><volume>26</volume><journalNumber>1</journalNumber><paginationStart>309</paginationStart><paginationEnd>323</paginationEnd><publisher>Springer Science and Business Media LLC</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>1265-4906</issnPrint><issnElectronic>1248-9204</issnElectronic><keywords>Foreign body reaction; Adaptive immune system; Innate immune system; Mesh; Fluorescence microscopy</keywords><publishedDay>1</publishedDay><publishedMonth>2</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-02-01</publishedDate><doi>10.1007/s10029-021-02396-7</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><funders>Open Access funding enabled and organized by Projekt DEAL. The support by the Federal Ministry of Education and Research (FKZ 13GW0108B) enabled the acquisition of the TissueGnostics system.</funders><projectreference/><lastEdited>2022-11-07T14:56:09.7458679</lastEdited><Created>2022-10-31T12:36:16.2087290</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>A.</firstname><surname>Dievernich</surname><orcid>0000-0001-6677-9801</orcid><order>1</order></author><author><firstname>P.</firstname><surname>Achenbach</surname><order>2</order></author><author><firstname>Luke</firstname><surname>Davies</surname><orcid>0000-0001-7767-4060</orcid><order>3</order></author><author><firstname>U.</firstname><surname>Klinge</surname><order>4</order></author></authors><documents><document><filename>61693__25676__29dcc5e49b244ef08abb1e89151a949a.pdf</filename><originalFilename>61693.pdf</originalFilename><uploaded>2022-11-07T14:54:56.7145960</uploaded><type>Output</type><contentLength>5620394</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2022-11-07T14:56:09.7458679 v2 61693 2022-10-31 Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen ff080296775381560053d5e3a6e81745 0000-0001-7767-4060 Luke Davies Luke Davies true false 2022-10-31 BMS BackgroundPolypropylene (PP) mesh is widely used to reinforce tissues. The foreign body reaction (FBR) to the implant is dominated by innate immune cells, especially macrophages. However, considerable numbers of adaptive immune cells, namely T cells, have also been regularly observed, which appear to play a crucial role in the long-term host response.MethodsThis study investigated the FBR to seven human PP meshes, which were removed from the abdomen for recurrence after a median of one year. Using immunofluorescence microscopy, the FBR was examined for various innate (CD11b+ myeloid, CD68+ macrophages, CD56+ NK) and adaptive immune cells (CD3+ T, CD4+ T-helper, CD8+ cytotoxic, FoxP3+ T-regulatory, CD20+ B) as well as “conventional” immune cells (defined as cells expressing their specific immune cell marker without co-expressing CD68).ResultsT-helper cells (19%) and regulatory T-cells (25%) were present at comparable rates to macrophages, and clustered significantly toward the mesh fibers. For all cell types the lowest proportions of “conventional” cells (< 60%) were observed at the mesh–tissue interface, but increased considerably at about 50–100 µm, indicating reduced stimulation with rising distance to the mesh fibers.ConclusionBoth innate and adaptive immune cells participate in the chronic FBR to PP meshes with T cells and macrophages being the predominant cell types, respectively. In concordance with the previous data, many cells presented a “hybrid” pattern near the mesh fibers. The complexity of the immune reaction seen within the foreign body granuloma may explain why approaches focusing on specific cell types have not been very successful in reducing the chronic FBR. Journal Article Hernia 26 1 309 323 Springer Science and Business Media LLC 1265-4906 1248-9204 Foreign body reaction; Adaptive immune system; Innate immune system; Mesh; Fluorescence microscopy 1 2 2022 2022-02-01 10.1007/s10029-021-02396-7 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Open Access funding enabled and organized by Projekt DEAL. The support by the Federal Ministry of Education and Research (FKZ 13GW0108B) enabled the acquisition of the TissueGnostics system. 2022-11-07T14:56:09.7458679 2022-10-31T12:36:16.2087290 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine A. Dievernich 0000-0001-6677-9801 1 P. Achenbach 2 Luke Davies 0000-0001-7767-4060 3 U. Klinge 4 61693__25676__29dcc5e49b244ef08abb1e89151a949a.pdf 61693.pdf 2022-11-07T14:54:56.7145960 Output 5620394 application/pdf Version of Record true © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/
title Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen
spellingShingle Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen
Luke Davies
title_short Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen
title_full Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen
title_fullStr Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen
title_full_unstemmed Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen
title_sort Characterization of innate and adaptive immune cells involved in the foreign body reaction to polypropylene meshes in the human abdomen
author_id_str_mv ff080296775381560053d5e3a6e81745
author_id_fullname_str_mv ff080296775381560053d5e3a6e81745_***_Luke Davies
author Luke Davies
author2 A. Dievernich
P. Achenbach
Luke Davies
U. Klinge
format Journal article
container_title Hernia
container_volume 26
container_issue 1
container_start_page 309
publishDate 2022
institution Swansea University
issn 1265-4906
1248-9204
doi_str_mv 10.1007/s10029-021-02396-7
publisher Springer Science and Business Media LLC
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description BackgroundPolypropylene (PP) mesh is widely used to reinforce tissues. The foreign body reaction (FBR) to the implant is dominated by innate immune cells, especially macrophages. However, considerable numbers of adaptive immune cells, namely T cells, have also been regularly observed, which appear to play a crucial role in the long-term host response.MethodsThis study investigated the FBR to seven human PP meshes, which were removed from the abdomen for recurrence after a median of one year. Using immunofluorescence microscopy, the FBR was examined for various innate (CD11b+ myeloid, CD68+ macrophages, CD56+ NK) and adaptive immune cells (CD3+ T, CD4+ T-helper, CD8+ cytotoxic, FoxP3+ T-regulatory, CD20+ B) as well as “conventional” immune cells (defined as cells expressing their specific immune cell marker without co-expressing CD68).ResultsT-helper cells (19%) and regulatory T-cells (25%) were present at comparable rates to macrophages, and clustered significantly toward the mesh fibers. For all cell types the lowest proportions of “conventional” cells (< 60%) were observed at the mesh–tissue interface, but increased considerably at about 50–100 µm, indicating reduced stimulation with rising distance to the mesh fibers.ConclusionBoth innate and adaptive immune cells participate in the chronic FBR to PP meshes with T cells and macrophages being the predominant cell types, respectively. In concordance with the previous data, many cells presented a “hybrid” pattern near the mesh fibers. The complexity of the immune reaction seen within the foreign body granuloma may explain why approaches focusing on specific cell types have not been very successful in reducing the chronic FBR.
published_date 2022-02-01T04:20:42Z
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