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Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata

Emma Quinn, Sophie Malkin, Jess Bevan, Andrew Rowley, Christopher Coates, Jessica Thomas

Diseases of Aquatic Organisms, Volume: 153, Pages: 69 - 79

Swansea University Authors: Emma Quinn, Sophie Malkin, Jess Bevan, Andrew Rowley, Christopher Coates, Jessica Thomas

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DOI (Published version): 10.3354/dao03722

Abstract

Two populations of the invasive slipper limpet Crepidula fornicata were sampled in Swansea Bay and Milford Haven, Wales, UK, to determine the presence of putative pathogens and parasites known to affect co-located commercially important shellfish (e.g. oysters). A multi-resource screen, including mo...

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Published in: Diseases of Aquatic Organisms
ISSN: 0177-5103 1616-1580
Published: Inter-Research Science Center 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa62916
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first_indexed 2023-03-12T15:50:58Z
last_indexed 2023-04-19T03:23:51Z
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spelling v2 62916 2023-03-12 Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata 6cb18d2ebc7cfec175374f0ff559d06b Emma Quinn Emma Quinn true false 17ff4136248e9382802ced1bc1d680e4 Sophie Malkin Sophie Malkin true false 11f13b14aed072e93e611759d9aa090f Jess Bevan Jess Bevan true false e98124f6e62b9592786899d7059e3a79 Andrew Rowley Andrew Rowley true false af160934b75bea5b8ba83d68b3d1a003 Christopher Coates Christopher Coates true false cf44490d4aff8772cf82b736998671ae Jessica Thomas Jessica Thomas true false 2023-03-12 SBI Two populations of the invasive slipper limpet Crepidula fornicata were sampled in Swansea Bay and Milford Haven, Wales, UK, to determine the presence of putative pathogens and parasites known to affect co-located commercially important shellfish (e.g. oysters). A multi-resource screen, including molecular and histological diagnoses, was used to assess 1800 individuals over 12 mo for microparasites, notably haplosporidians, microsporidians and paramyxids. Although initial PCR-based methods suggested the presence of these microparasites, there was no evidence of infection when assessed histologically, or when all PCR amplicons (n = 294) were sequenced. Whole tissue histology of 305 individuals revealed turbellarians in the lumen of the alimentary canal, in addition to unusual cells of unknown origin in the epithelial lining. In total, 6% of C. fornicata screened histologically harboured turbellarians, and approximately 33% contained the abnormal cells—so named due to their altered cytoplasm and condensed chromatin. A small number of limpets (~1%) also had pathologies in the digestive gland including tubule necrosis, haemocytic infiltration and sloughed cells in the tubule lumen. Overall, these data suggest that C. fornicata are not susceptible to substantive infections by microparasites outside of their native range, which may contribute in part to their invasion success. Journal Article Diseases of Aquatic Organisms 153 69 79 Inter-Research Science Center 0177-5103 1616-1580 Turbellarians, False positive PCR, Microparasites, Nuclear aberrations, Pathology, Sepsis 2 3 2023 2023-03-02 10.3354/dao03722 http://dx.doi.org/10.3354/dao03722 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University Another institution paid the OA fee EU ERDF Ireland Wales Bluefish 2023-06-21T09:44:38.7480600 2023-03-12T15:48:28.6866656 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Emma Quinn 1 Sophie Malkin 2 Jess Bevan 3 Andrew Rowley 4 Christopher Coates 5 Jessica Thomas 6
title Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata
spellingShingle Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata
Emma Quinn
Sophie Malkin
Jess Bevan
Andrew Rowley
Christopher Coates
Jessica Thomas
title_short Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata
title_full Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata
title_fullStr Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata
title_full_unstemmed Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata
title_sort Histopathological survey of putative parasites and pathogens in non-native slipper limpets Crepidula fornicata
author_id_str_mv 6cb18d2ebc7cfec175374f0ff559d06b
17ff4136248e9382802ced1bc1d680e4
11f13b14aed072e93e611759d9aa090f
e98124f6e62b9592786899d7059e3a79
af160934b75bea5b8ba83d68b3d1a003
cf44490d4aff8772cf82b736998671ae
author_id_fullname_str_mv 6cb18d2ebc7cfec175374f0ff559d06b_***_Emma Quinn
17ff4136248e9382802ced1bc1d680e4_***_Sophie Malkin
11f13b14aed072e93e611759d9aa090f_***_Jess Bevan
e98124f6e62b9592786899d7059e3a79_***_Andrew Rowley
af160934b75bea5b8ba83d68b3d1a003_***_Christopher Coates
cf44490d4aff8772cf82b736998671ae_***_Jessica Thomas
author Emma Quinn
Sophie Malkin
Jess Bevan
Andrew Rowley
Christopher Coates
Jessica Thomas
author2 Emma Quinn
Sophie Malkin
Jess Bevan
Andrew Rowley
Christopher Coates
Jessica Thomas
format Journal article
container_title Diseases of Aquatic Organisms
container_volume 153
container_start_page 69
publishDate 2023
institution Swansea University
issn 0177-5103
1616-1580
doi_str_mv 10.3354/dao03722
publisher Inter-Research Science Center
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
url http://dx.doi.org/10.3354/dao03722
document_store_str 0
active_str 0
description Two populations of the invasive slipper limpet Crepidula fornicata were sampled in Swansea Bay and Milford Haven, Wales, UK, to determine the presence of putative pathogens and parasites known to affect co-located commercially important shellfish (e.g. oysters). A multi-resource screen, including molecular and histological diagnoses, was used to assess 1800 individuals over 12 mo for microparasites, notably haplosporidians, microsporidians and paramyxids. Although initial PCR-based methods suggested the presence of these microparasites, there was no evidence of infection when assessed histologically, or when all PCR amplicons (n = 294) were sequenced. Whole tissue histology of 305 individuals revealed turbellarians in the lumen of the alimentary canal, in addition to unusual cells of unknown origin in the epithelial lining. In total, 6% of C. fornicata screened histologically harboured turbellarians, and approximately 33% contained the abnormal cells—so named due to their altered cytoplasm and condensed chromatin. A small number of limpets (~1%) also had pathologies in the digestive gland including tubule necrosis, haemocytic infiltration and sloughed cells in the tubule lumen. Overall, these data suggest that C. fornicata are not susceptible to substantive infections by microparasites outside of their native range, which may contribute in part to their invasion success.
published_date 2023-03-02T09:44:37Z
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score 10.998207

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