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A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages

Thomas Jovic, Tamsin Nicholson, Hari Arora Orcid Logo, Kim Nelson, Shareen Doak Orcid Logo, Iain Whitaker

Carbohydrate Polymers, Volume: 321, Start page: 121261

Swansea University Authors: Thomas Jovic, Hari Arora Orcid Logo, Shareen Doak Orcid Logo, Iain Whitaker

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DOI (Published version): 10.1016/j.carbpol.2023.121261

Abstract

Nanocelluloses have attracted significant interest in the field of bioprinting, with previous research outlining the value of nanocellulose fibrils and bacterial nanocelluloses for 3D bioprinting tissues such as cartilage. We have recently characterised three distinct structural formulations of pulp...

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Published in: Carbohydrate Polymers
ISSN: 0144-8617 1879-1344
Published: Elsevier BV 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa64062
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We have recently characterised three distinct structural formulations of pulp-derived nanocelluloses: fibrillar (NFC), crystalline (NCC) and blend (NCB), exhibiting variation in pore geometry and mechanical properties. In light of the characterisation of these three distinct entities, this study investigated whether these structural differences translated to differences in printability, chondrogenicity or biocompatibility for 3D bioprinting anatomical structures with human nasoseptal chondrocytes. Composite nanocellulose-alginate bioinks (75:25 v/v) of NFC, NCC and NCB were produced and tested for print resolution and fidelity. NFC offered superior print resolution whereas NCB demonstrated the best post-printing shape fidelity. Biologically, chondrogenicity was assessed using real time quantitative PCR, dimethylmethylene blue assays and histology. 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Nanocellulose augments printability and chondrogenicity of bioinks, of which the NCC and NCB formulations offer the best biological promise for bioprinting cartilage.</abstract><type>Journal Article</type><journal>Carbohydrate Polymers</journal><volume>321</volume><journalNumber/><paginationStart>121261</paginationStart><paginationEnd/><publisher>Elsevier BV</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>0144-8617</issnPrint><issnElectronic>1879-1344</issnElectronic><keywords>Cartilage, Bioprinting, Nanocellulose, Alginate</keywords><publishedDay>1</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2023</publishedYear><publishedDate>2023-12-01</publishedDate><doi>10.1016/j.carbpol.2023.121261</doi><url>http://dx.doi.org/10.1016/j.carbpol.2023.121261</url><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm>SU Library paid the OA fee (TA Institutional Deal)</apcterm><funders>This study was supported by The Scar Free Foundation &amp; Health and Care Research Wales Programme of research in Reconstructive Surgery &amp; Regenerative Medicine, which has been established in the ReconRegen Research Centre at Swansea University in partnership with Swansea Bay University Health Board. 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spelling v2 64062 2023-08-09 A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages 7d95ed2bceb18fc0fdfd4048277c6eed Thomas Jovic Thomas Jovic true false ed7371c768e9746008a6807f9f7a1555 0000-0002-9790-0907 Hari Arora Hari Arora true false 8f70286908f67238a527a98cbf66d387 0000-0002-6753-1987 Shareen Doak Shareen Doak true false 830074c59291938a55b480dcbee4697e Iain Whitaker Iain Whitaker true false 2023-08-09 BMS Nanocelluloses have attracted significant interest in the field of bioprinting, with previous research outlining the value of nanocellulose fibrils and bacterial nanocelluloses for 3D bioprinting tissues such as cartilage. We have recently characterised three distinct structural formulations of pulp-derived nanocelluloses: fibrillar (NFC), crystalline (NCC) and blend (NCB), exhibiting variation in pore geometry and mechanical properties. In light of the characterisation of these three distinct entities, this study investigated whether these structural differences translated to differences in printability, chondrogenicity or biocompatibility for 3D bioprinting anatomical structures with human nasoseptal chondrocytes. Composite nanocellulose-alginate bioinks (75:25 v/v) of NFC, NCC and NCB were produced and tested for print resolution and fidelity. NFC offered superior print resolution whereas NCB demonstrated the best post-printing shape fidelity. Biologically, chondrogenicity was assessed using real time quantitative PCR, dimethylmethylene blue assays and histology. All biomaterials showed an increase in chondrogenic gene expression and extracellular matrix production over 21 days, but this was superior in the NCC bioink. Biocompatibility assessments revealed an increase in cell number and metabolism over 21 days in the NCC and NCB formulations. Nanocellulose augments printability and chondrogenicity of bioinks, of which the NCC and NCB formulations offer the best biological promise for bioprinting cartilage. Journal Article Carbohydrate Polymers 321 121261 Elsevier BV 0144-8617 1879-1344 Cartilage, Bioprinting, Nanocellulose, Alginate 1 12 2023 2023-12-01 10.1016/j.carbpol.2023.121261 http://dx.doi.org/10.1016/j.carbpol.2023.121261 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University SU Library paid the OA fee (TA Institutional Deal) This study was supported by The Scar Free Foundation & Health and Care Research Wales Programme of research in Reconstructive Surgery & Regenerative Medicine, which has been established in the ReconRegen Research Centre at Swansea University in partnership with Swansea Bay University Health Board. This work was additionally supported by Action Medical Research and the VTCT Foundation (Grant Number 2782), the Royal College of Surgeons England and the Welsh Clinical Academic Training Programme. 2024-02-02T08:24:15.8300598 2023-08-09T09:44:38.1164207 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Thomas Jovic 1 Tamsin Nicholson 2 Hari Arora 0000-0002-9790-0907 3 Kim Nelson 4 Shareen Doak 0000-0002-6753-1987 5 Iain Whitaker 6 64062__28293__42889230bcd446c384adc224b02049e8.pdf 64062.VOR.pdf 2023-08-14T11:01:54.2490583 Output 8103164 application/pdf Version of Record true © 2023 The Authors. Published by Elsevier Ltd. Distributed under the terms of a Creative Commons Attribution 4.0 License (CC BY 4.0). true eng https://creativecommons.org/licenses/by/4.0/
title A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
spellingShingle A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
Thomas Jovic
Hari Arora
Shareen Doak
Iain Whitaker
title_short A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
title_full A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
title_fullStr A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
title_full_unstemmed A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
title_sort A comparative analysis of pulp-derived nanocelluloses for 3D bioprinting facial cartilages
author_id_str_mv 7d95ed2bceb18fc0fdfd4048277c6eed
ed7371c768e9746008a6807f9f7a1555
8f70286908f67238a527a98cbf66d387
830074c59291938a55b480dcbee4697e
author_id_fullname_str_mv 7d95ed2bceb18fc0fdfd4048277c6eed_***_Thomas Jovic
ed7371c768e9746008a6807f9f7a1555_***_Hari Arora
8f70286908f67238a527a98cbf66d387_***_Shareen Doak
830074c59291938a55b480dcbee4697e_***_Iain Whitaker
author Thomas Jovic
Hari Arora
Shareen Doak
Iain Whitaker
author2 Thomas Jovic
Tamsin Nicholson
Hari Arora
Kim Nelson
Shareen Doak
Iain Whitaker
format Journal article
container_title Carbohydrate Polymers
container_volume 321
container_start_page 121261
publishDate 2023
institution Swansea University
issn 0144-8617
1879-1344
doi_str_mv 10.1016/j.carbpol.2023.121261
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
url http://dx.doi.org/10.1016/j.carbpol.2023.121261
document_store_str 1
active_str 0
description Nanocelluloses have attracted significant interest in the field of bioprinting, with previous research outlining the value of nanocellulose fibrils and bacterial nanocelluloses for 3D bioprinting tissues such as cartilage. We have recently characterised three distinct structural formulations of pulp-derived nanocelluloses: fibrillar (NFC), crystalline (NCC) and blend (NCB), exhibiting variation in pore geometry and mechanical properties. In light of the characterisation of these three distinct entities, this study investigated whether these structural differences translated to differences in printability, chondrogenicity or biocompatibility for 3D bioprinting anatomical structures with human nasoseptal chondrocytes. Composite nanocellulose-alginate bioinks (75:25 v/v) of NFC, NCC and NCB were produced and tested for print resolution and fidelity. NFC offered superior print resolution whereas NCB demonstrated the best post-printing shape fidelity. Biologically, chondrogenicity was assessed using real time quantitative PCR, dimethylmethylene blue assays and histology. All biomaterials showed an increase in chondrogenic gene expression and extracellular matrix production over 21 days, but this was superior in the NCC bioink. Biocompatibility assessments revealed an increase in cell number and metabolism over 21 days in the NCC and NCB formulations. Nanocellulose augments printability and chondrogenicity of bioinks, of which the NCC and NCB formulations offer the best biological promise for bioprinting cartilage.
published_date 2023-12-01T08:24:15Z
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