Journal article 223 views
Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
Javad Sahandi 
,
Patrick Sorgeloos,
Kam Tang ,
Fanghong Mu,
Tatyana Mayor,
Wenbing Zhang
,
Patrick Sorgeloos,
Kam Tang ,
Fanghong Mu,
Tatyana Mayor,
Wenbing Zhang
Microbial Pathogenesis, Volume: 183, Start page: 106334
Swansea University Author:
Kam Tang
DOI (Published version): 10.1016/j.micpath.2023.106334
Abstract
The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum...
| Published in: | Microbial Pathogenesis |
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| ISSN: | 0882-4010 1096-1208 |
| Published: |
Elsevier BV
2023
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa64470 |
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| Abstract: |
The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum, and Bacillus subtilis, to suppress Vibrio and increase survival, population growth and digestive enzyme activity (protease, lipase, and amylase) in the harpacticoid copepod, Tigriopus japonicus. Copepod, T. japonicus stock culture with an initial mean density of 50 individual/mL (25 adult male and 25 adult female) was distributed into five treatments (i.e., four experimental and a control, each with four replicates; repeated twice) using 20 beakers (100 mL capacity each). The copepods were fed a mixture of the dinoflagellate Alexandrium tamarense and the diatom Phyaeodactylum tricornutum (3 ×104 cells/mL 1). Each microbe’s concentration was adjusted at 108 CFU/mL 1 and applied to the culture condition. D. hansenii, L. plantarum, and B. subtilis all improved the copepods’ survival and population growth, likely by including a higher lipase activity (P < 0.05). In contrast, using R. mobilis did not improve the copepod’s culture performance compared to control. B. subtilis was the most effective in decreasing the copepod’s external and internal Vibrio loading. The probiotic concentrations in the copepod decreased within days during starvation, suggesting that routine re-application of the probiotics would be needed to sustain the microbial populations and the benefits they provide. Our results demonstrated that D. hansenii and B. subtilis are promising probiotics formass copepod culture as live food for mariculture purposes. |
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| Keywords: |
Bacillus subtilis, Copepod, Debaryomyces hansenii, Larvae, Microbe, Tigriopus japonicus |
| College: |
Faculty of Science and Engineering |
| Start Page: |
106334 |