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Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori

Javad Sahandi Orcid Logo, Patrick Sorgeloos, Kam Tang Orcid Logo, Fanghong Mu, Tatyana Mayor, Wenbing Zhang

Microbial Pathogenesis, Volume: 183, Start page: 106334

Swansea University Author: Kam Tang Orcid Logo

  • Accepted Manuscript under embargo until: 7th September 2024

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DOI (Published version): 10.1016/j.micpath.2023.106334

Abstract

The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum...

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Published in: Microbial Pathogenesis
ISSN: 0882-4010 1096-1208
Published: Elsevier BV 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa64470
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spelling v2 64470 2023-09-08 Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori 69af43a3b9da24aef65c5d3a44956fe3 0000-0001-9427-9564 Kam Tang Kam Tang true false 2023-09-08 SBI The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum, and Bacillus subtilis, to suppress Vibrio and increase survival, population growth and digestive enzyme activity (protease, lipase, and amylase) in the harpacticoid copepod, Tigriopus japonicus. Copepod, T. japonicus stock culture with an initial mean density of 50 individual/mL (25 adult male and 25 adult female) was distributed into five treatments (i.e., four experimental and a control, each with four replicates; repeated twice) using 20 beakers (100 mL capacity each). The copepods were fed a mixture of the dinoflagellate Alexandrium tamarense and the diatom Phyaeodactylum tricornutum (3 ×104 cells/mL 1). Each microbe’s concentration was adjusted at 108 CFU/mL 1 and applied to the culture condition. D. hansenii, L. plantarum, and B. subtilis all improved the copepods’ survival and population growth, likely by including a higher lipase activity (P < 0.05). In contrast, using R. mobilis did not improve the copepod’s culture performance compared to control. B. subtilis was the most effective in decreasing the copepod’s external and internal Vibrio loading. The probiotic concentrations in the copepod decreased within days during starvation, suggesting that routine re-application of the probiotics would be needed to sustain the microbial populations and the benefits they provide. Our results demonstrated that D. hansenii and B. subtilis are promising probiotics formass copepod culture as live food for mariculture purposes. Journal Article Microbial Pathogenesis 183 106334 Elsevier BV 0882-4010 1096-1208 Bacillus subtilis, Copepod, Debaryomyces hansenii, Larvae, Microbe, Tigriopus japonicus 31 10 2023 2023-10-31 10.1016/j.micpath.2023.106334 http://dx.doi.org/10.1016/j.micpath.2023.106334 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University Not Required 2023-10-09T16:19:42.0739506 2023-09-08T08:37:27.0127630 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Javad Sahandi 0000-0001-8403-3480 1 Patrick Sorgeloos 2 Kam Tang 0000-0001-9427-9564 3 Fanghong Mu 4 Tatyana Mayor 5 Wenbing Zhang 6 Under embargo Under embargo 2023-09-08T08:39:59.3723497 Output 622366 application/pdf Accepted Manuscript true 2024-09-07T00:00:00.0000000 false eng
title Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
spellingShingle Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
Kam Tang
title_short Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
title_full Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
title_fullStr Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
title_full_unstemmed Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
title_sort Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori
author_id_str_mv 69af43a3b9da24aef65c5d3a44956fe3
author_id_fullname_str_mv 69af43a3b9da24aef65c5d3a44956fe3_***_Kam Tang
author Kam Tang
author2 Javad Sahandi
Patrick Sorgeloos
Kam Tang
Fanghong Mu
Tatyana Mayor
Wenbing Zhang
format Journal article
container_title Microbial Pathogenesis
container_volume 183
container_start_page 106334
publishDate 2023
institution Swansea University
issn 0882-4010
1096-1208
doi_str_mv 10.1016/j.micpath.2023.106334
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
url http://dx.doi.org/10.1016/j.micpath.2023.106334
document_store_str 0
active_str 0
description The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum, and Bacillus subtilis, to suppress Vibrio and increase survival, population growth and digestive enzyme activity (protease, lipase, and amylase) in the harpacticoid copepod, Tigriopus japonicus. Copepod, T. japonicus stock culture with an initial mean density of 50 individual/mL (25 adult male and 25 adult female) was distributed into five treatments (i.e., four experimental and a control, each with four replicates; repeated twice) using 20 beakers (100 mL capacity each). The copepods were fed a mixture of the dinoflagellate Alexandrium tamarense and the diatom Phyaeodactylum tricornutum (3 ×104 cells/mL 1). Each microbe’s concentration was adjusted at 108 CFU/mL 1 and applied to the culture condition. D. hansenii, L. plantarum, and B. subtilis all improved the copepods’ survival and population growth, likely by including a higher lipase activity (P < 0.05). In contrast, using R. mobilis did not improve the copepod’s culture performance compared to control. B. subtilis was the most effective in decreasing the copepod’s external and internal Vibrio loading. The probiotic concentrations in the copepod decreased within days during starvation, suggesting that routine re-application of the probiotics would be needed to sustain the microbial populations and the benefits they provide. Our results demonstrated that D. hansenii and B. subtilis are promising probiotics formass copepod culture as live food for mariculture purposes.
published_date 2023-10-31T16:19:43Z
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