Journal article 154 views 26 downloads
How pure are your vesicles?
,
Aled Clayton
Journal of Extracellular Vesicles, Volume: 2, Issue: 1
Swansea University Author:
Jason Webber
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© 2013 Jason Webber and Aled Clayton. Distributed under the terms of a Creative Commons Attribution-NonCommercial 3.0 Unported License (CC BY-NC 3.0).
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DOI (Published version): 10.3402/jev.v2i0.19861
Abstract
We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is sim...
| Published in: | Journal of Extracellular Vesicles |
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| ISSN: | 2001-3078 2001-3078 |
| Published: |
Wiley
2013
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa64725 |
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| Abstract: |
We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles. |
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| Keywords: |
Extracellular vesicles, nano-particle tracking, vesicle counting, sample purity |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This work was supported by a Cancer Research Wales programme grant and an award from the Movember GAP1 Exosome Biomarker study to AC. |
| Issue: |
1 |