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How pure are your vesicles?

Jason Webber Orcid Logo, Aled Clayton

Journal of Extracellular Vesicles, Volume: 2, Issue: 1

Swansea University Author: Jason Webber Orcid Logo

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DOI (Published version): 10.3402/jev.v2i0.19861

Abstract

We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is sim...

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Published in: Journal of Extracellular Vesicles
ISSN: 2001-3078 2001-3078
Published: Wiley 2013
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URI: https://cronfa.swan.ac.uk/Record/cronfa64725
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first_indexed 2023-10-11T15:47:24Z
last_indexed 2023-10-11T15:47:24Z
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spelling v2 64725 2023-10-11 How pure are your vesicles? 25d1a26f9b8bb556bd9412080e40351d 0000-0003-4772-3014 Jason Webber Jason Webber true false 2023-10-11 BMS We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles. Journal Article Journal of Extracellular Vesicles 2 1 Wiley 2001-3078 2001-3078 Extracellular vesicles, nano-particle tracking, vesicle counting, sample purity 14 1 2013 2013-01-14 10.3402/jev.v2i0.19861 http://dx.doi.org/10.3402/jev.v2i0.19861 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Another institution paid the OA fee This work was supported by a Cancer Research Wales programme grant and an award from the Movember GAP1 Exosome Biomarker study to AC. 2023-11-28T10:51:43.0309872 2023-10-11T16:46:34.9344657 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Jason Webber 0000-0003-4772-3014 1 Aled Clayton 2 64725__29128__479b1873ef08411aad32ce2e6b29294b.pdf 64725.VOR.pdf 2023-11-28T10:49:34.7221676 Output 1528070 application/pdf Version of Record true © 2013 Jason Webber and Aled Clayton. Distributed under the terms of a Creative Commons Attribution-NonCommercial 3.0 Unported License (CC BY-NC 3.0). true eng https://creativecommons.org/licenses/by-nc/3.0/
title How pure are your vesicles?
spellingShingle How pure are your vesicles?
Jason Webber
title_short How pure are your vesicles?
title_full How pure are your vesicles?
title_fullStr How pure are your vesicles?
title_full_unstemmed How pure are your vesicles?
title_sort How pure are your vesicles?
author_id_str_mv 25d1a26f9b8bb556bd9412080e40351d
author_id_fullname_str_mv 25d1a26f9b8bb556bd9412080e40351d_***_Jason Webber
author Jason Webber
author2 Jason Webber
Aled Clayton
format Journal article
container_title Journal of Extracellular Vesicles
container_volume 2
container_issue 1
publishDate 2013
institution Swansea University
issn 2001-3078
2001-3078
doi_str_mv 10.3402/jev.v2i0.19861
publisher Wiley
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
url http://dx.doi.org/10.3402/jev.v2i0.19861
document_store_str 1
active_str 0
description We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.
published_date 2013-01-14T10:51:43Z
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score 11.03559

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