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A rapid and general method for measurement of protein in micro-algal biomass

Steve Slocombe, Michael Ross, Naomi Thomas, Sharon McNeill, Michele S. Stanley

Bioresource Technology, Volume: 129, Pages: 51 - 57

Swansea University Author: Steve Slocombe

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DOI (Published version): 10.1016/j.biortech.2012.10.163

Abstract

A convenient small-scale extraction method for lyophilized micro-algae is described that dispenses with labor-intensive homogenization and is widely applicable to algae from different phyla. The procedure employs an optimized sequential extraction in trichloroacetic acid (TCA) and NaOH to achieve ch...

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Published in: Bioresource Technology
ISSN: 0960-8524
Published: Elsevier BV 2013
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URI: https://cronfa.swan.ac.uk/Record/cronfa65476
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first_indexed 2024-01-22T13:10:22Z
last_indexed 2024-01-22T13:10:22Z
id cronfa65476
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spelling v2 65476 2024-01-22 A rapid and general method for measurement of protein in micro-algal biomass 4a1ea486a78ed357efdfa053a277ae40 Steve Slocombe Steve Slocombe true false 2024-01-22 SBI A convenient small-scale extraction method for lyophilized micro-algae is described that dispenses with labor-intensive homogenization and is widely applicable to algae from different phyla. The procedure employs an optimized sequential extraction in trichloroacetic acid (TCA) and NaOH to achieve chemical lysis. Conditions were tested using several micro-algal strains to develop a method that was generally applicable. Incubation of lyophilized material in 24% (w/v) TCA at 95 °C followed by a hot alkaline treatment was found to be effective for strains that are resistant to conventional extraction approaches, such as the Chlorella and the Eustigmatophycean species. The single-tube extraction procedure can be complete in 4h and is conveniently followed by the Lowry assay, requiring a further 30 min. Overall, this method proved to be generally applicable and ideal either for single samples or for high-throughput screening of multiple algal strains for protein content. Journal Article Bioresource Technology 129 51 57 Elsevier BV 0960-8524 Intelligent screening; Protein extraction; Value-added products; Algal biomass; Aquaculture 1 2 2013 2013-02-01 10.1016/j.biortech.2012.10.163 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University 2024-03-23T11:37:09.1743859 2024-01-22T13:09:38.2828565 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Steve Slocombe 1 Michael Ross 2 Naomi Thomas 3 Sharon McNeill 4 Michele S. Stanley 5
title A rapid and general method for measurement of protein in micro-algal biomass
spellingShingle A rapid and general method for measurement of protein in micro-algal biomass
Steve Slocombe
title_short A rapid and general method for measurement of protein in micro-algal biomass
title_full A rapid and general method for measurement of protein in micro-algal biomass
title_fullStr A rapid and general method for measurement of protein in micro-algal biomass
title_full_unstemmed A rapid and general method for measurement of protein in micro-algal biomass
title_sort A rapid and general method for measurement of protein in micro-algal biomass
author_id_str_mv 4a1ea486a78ed357efdfa053a277ae40
author_id_fullname_str_mv 4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
author Steve Slocombe
author2 Steve Slocombe
Michael Ross
Naomi Thomas
Sharon McNeill
Michele S. Stanley
format Journal article
container_title Bioresource Technology
container_volume 129
container_start_page 51
publishDate 2013
institution Swansea University
issn 0960-8524
doi_str_mv 10.1016/j.biortech.2012.10.163
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 0
active_str 0
description A convenient small-scale extraction method for lyophilized micro-algae is described that dispenses with labor-intensive homogenization and is widely applicable to algae from different phyla. The procedure employs an optimized sequential extraction in trichloroacetic acid (TCA) and NaOH to achieve chemical lysis. Conditions were tested using several micro-algal strains to develop a method that was generally applicable. Incubation of lyophilized material in 24% (w/v) TCA at 95 °C followed by a hot alkaline treatment was found to be effective for strains that are resistant to conventional extraction approaches, such as the Chlorella and the Eustigmatophycean species. The single-tube extraction procedure can be complete in 4h and is conveniently followed by the Lowry assay, requiring a further 30 min. Overall, this method proved to be generally applicable and ideal either for single samples or for high-throughput screening of multiple algal strains for protein content.
published_date 2013-02-01T11:37:06Z
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score 11.035765

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