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Unveiling the potential of Daldinia eschscholtzii MFLUCC 19-0629 through bioactivity and bioinformatics studies for enhanced sustainable agriculture production
Frontiers in Chemical Biology, Volume: 3
Swansea University Authors:
Shumukh Alharthi, Claudio Greco
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© 2024 Brooks, Weaver, Klomchit, Alharthi, Onlamun, Nurani, Vong, Alberti and Greco. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
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DOI (Published version): 10.3389/fchbi.2024.1362147
Abstract
Endophytic fungi constitute a rich source of secondary metabolites that can be manipulated to produce desirable novel analogs for combating current agricultural challenges for crop production, especially controlling plant disease. The endophytic fungus Daldinia eschscholtzii MFLUCC 19-0629, was newl...
| Published in: | Frontiers in Chemical Biology |
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| ISSN: | 2813-530X |
| Published: |
Frontiers Media SA
2024
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa66224 |
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| Abstract: |
Endophytic fungi constitute a rich source of secondary metabolites that can be manipulated to produce desirable novel analogs for combating current agricultural challenges for crop production, especially controlling plant disease. The endophytic fungus Daldinia eschscholtzii MFLUCC 19-0629, was newly isolated from tropical ancient plants, Oncosperma sp., and displays a broad-spectrum of antifungal and antibacterial activities against several plant pathogens including Ralstonia solanacearum, Fusarium oxysporum, Colletotrichum gloeosporioides, Colletotrichum acutatum, Stagonosporopsis cucurbitacearum, Corynespora cassiicola and Stemphylium spp. A high-quality genome sequence was obtained using Oxford nanopore technology, the accuracy and length of reads resulting in no need for Illumina or other sequencing techniques, for D. eschscholtzii MFLUCC 19-0629, resulting in a genome size of 37.56 Mb assembled over 11 contigs of significant size, likely to be at the chromosomal level. Bioinformatics analysis revealed that this strain is biosynthetically talented encoding 67 predicted biosynthetic gene clusters (BGCs). Only eight of the 67 BGCs matched or demonstrated high similarity to previously characterized BGCs linked to the production of known secondary metabolites. The high number of predicted unknown BGCs makes this strain a promising source of novel natural products. The discovery that D. eschscholtzii MFLUCC 19-0629 has a broad spectrum of antimicrobial activity against seven major plant pathogenic microorganisms relevant to crop production and its complete genome sequence carries immense importance in the advancement of novel microbial biocontrol agents (MBCAs). This also unveils the prospect of uncovering new compounds that could be utilized for sustainable agriculture and pharmaceutical purposes. |
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| Keywords: |
secondary metabolites, fungal endophytes, biosynthetic gene clusters, microbial biocontrol agents, Daldinia, genome |
| College: |
Faculty of Science and Engineering |
| Funders: |
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. CG was supported by the BBSRC BB/V005723/2. SA’s PhD Scholarship was supported by Taif University. JW was supported by a scholarship from the Engineering and Physical Sciences Research Council and the Biotechnology and Biological Sciences Research Council (EP/L016494/1) through the Centre for Doctoral Training in Synthetic Biology (SynBioCDT). FA was supported by a UKRI Future Leaders Fellowship (MR/V022334/1). SB was supported by Mae Fah Luang University under the grant name “Reinventing University 2021” and Minister of Higher Education, Science, Research and Innovation under grant name “Talent Mobility 2023 (TM65005).” |