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Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species

Anthikan Klomchit, Mark S Calabon, Sompradtana Worabandit, Jack A Weaver, Elfina Karima, Fabrizio Alberti, Claudio Greco Orcid Logo, Siraprapa Mahanil Orcid Logo

Journal of Applied Microbiology, Volume: 135, Issue: 5

Swansea University Authors: Elfina Karima, Claudio Greco Orcid Logo

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DOI (Published version): 10.1093/jambio/lxae114

Abstract

AimsNeocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phyl...

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Published in: Journal of Applied Microbiology
ISSN: 1365-2672
Published: Oxford University Press (OUP) 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa66499
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Abstract: AimsNeocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogeny, bioactivity screening, genome sequencing, and bioinformatics analysis.Methods and resultsDetailed descriptions, illustrations, and a multi-locus phylogenetic tree with large subunit ribosomal DNA (LSU), internal transcribed spacer (ITS), translation elongation factor 1-alpha (ef1-α), and RNA polymerase II second largest subunit (RPB2) regions showing the placement of three fungal strains, MFLUCC 17–0253, MFLUCC 17–0257, and MFLUCC 17–0259 clustered within the Neocosmospora clade with strong statistical support. Fungal crude extracts of the new species N. mangrovei MFLUCC 17–0253 exhibited strong antifungal activity to control Colletotrichum truncatum CG-0064, while N. ferruginea MFLUCC 17–0259 exhibited only moderate antifungal activity toward C. acutatum CC-0036. Thus, N. mangrovei MFLUCC 17–0253 was sequenced by Oxford nanopore technology. The bioinformatics analysis revealed that 49.17 Mb genome of this fungus harbors 41 potential biosynthetic gene clusters.ConclusionTwo fungal isolates of Neocosmospora and a new species of N. mangrovei were reported in this study. These fungal strains showed activity against pathogenic fungi causing anthracnose in chili. In addition, full genome sequencing and bioinformatics analysis of N. mangrovei MFLUCC 17–0253 were obtained.
Keywords: one new taxon, bioactivity, mangrove fungi, multi-locus phylogenetic analyses, whole genome, biosynthetic gene clusters
College: Faculty of Science and Engineering
Funders: C. Greco was supported by the BBSRC (BB/V005723/2). E. Karima’s PhD Scholarship was supported by EPSRC/Swansea University (EP/W524694/1). Jack Weaver was supported by a scholarship from the Engineering and Physical Sciences Re- search Council and the Biotechnology and Biological Sci- ences Research Council [EP/L016494/1] through the Centre for Doctoral Training in Synthetic Biology (SynBioCDT). Fab- rizio Alberti was supported by a UKRI Future Leaders Fellow- ship [MR/V022334/1]. Siraprapa Mahanil was supported by Mae Fah Luang University under the grant name “Reinvent- ing University 2021.”
Issue: 5

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